Flow cytometric quantitation of oxidative product formation by heterophils from orange-winged Amazon parrots (Amazona amazonica amazonica)

Lisa A Tell, M. B. Kabbur, W. L. Smith, James S Cullor

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Oxidative metabolic burst activity by heterophils from orange-winged Amazon parrots (Amazona amazonica amazonica) was indirectly evaluated by measuring the oxidation of non-fluorescent intracellular 2',7'-dichlorofluorescin (DCFH) to fluorescent 2',7/dichlorofluorescein (DCF) with flow cytometry. Heterophils were isolated from whole blood samples by preconcentrating the white blood cells with 3% hetastarch before running the samples through a polysucrose and disodium diatrizoate centrifugation. Phorbol myristate acetate (PMA) was used to activate the heterophils. Results show that heterophils from orange-winged Amazon parrots can oxidise DCFH to a fluorescent product and a wide range of DCF production was observed among individual birds. The production of DCF increased with PMA concentrations and significant differences were seen between the highest and lowest concentrations of PMA. These results support the concept that heterophils from orange-winged Amazon parrots can produce oxidative metabolites and that these heterophils appear to have functional heterogenicity.

Original languageEnglish (US)
Pages (from-to)197-201
Number of pages5
JournalComparative Haematology International
Volume7
Issue number4
StatePublished - 1997

Fingerprint

Amazona
Tetradecanoylphorbol Acetate
Hydroxyethyl Starch Derivatives
Diatrizoate
Respiratory Burst
Centrifugation
Birds
Flow Cytometry
Leukocytes

Keywords

  • 2',7'-Dichlorofluorescein (DCF)
  • 2',7'-Dichlorofluorescin (DCFH)
  • Flow cytometry
  • Heterophil
  • Orange-winged Amazon parrot

ASJC Scopus subject areas

  • Hematology

Cite this

@article{40af92c8e2d441b988d99f499737b3b0,
title = "Flow cytometric quantitation of oxidative product formation by heterophils from orange-winged Amazon parrots (Amazona amazonica amazonica)",
abstract = "Oxidative metabolic burst activity by heterophils from orange-winged Amazon parrots (Amazona amazonica amazonica) was indirectly evaluated by measuring the oxidation of non-fluorescent intracellular 2',7'-dichlorofluorescin (DCFH) to fluorescent 2',7/dichlorofluorescein (DCF) with flow cytometry. Heterophils were isolated from whole blood samples by preconcentrating the white blood cells with 3{\%} hetastarch before running the samples through a polysucrose and disodium diatrizoate centrifugation. Phorbol myristate acetate (PMA) was used to activate the heterophils. Results show that heterophils from orange-winged Amazon parrots can oxidise DCFH to a fluorescent product and a wide range of DCF production was observed among individual birds. The production of DCF increased with PMA concentrations and significant differences were seen between the highest and lowest concentrations of PMA. These results support the concept that heterophils from orange-winged Amazon parrots can produce oxidative metabolites and that these heterophils appear to have functional heterogenicity.",
keywords = "2',7'-Dichlorofluorescein (DCF), 2',7'-Dichlorofluorescin (DCFH), Flow cytometry, Heterophil, Orange-winged Amazon parrot",
author = "Tell, {Lisa A} and Kabbur, {M. B.} and Smith, {W. L.} and Cullor, {James S}",
year = "1997",
language = "English (US)",
volume = "7",
pages = "197--201",
journal = "Comparative Clinical Pathology",
issn = "1618-5641",
publisher = "Springer London",
number = "4",

}

TY - JOUR

T1 - Flow cytometric quantitation of oxidative product formation by heterophils from orange-winged Amazon parrots (Amazona amazonica amazonica)

AU - Tell, Lisa A

AU - Kabbur, M. B.

AU - Smith, W. L.

AU - Cullor, James S

PY - 1997

Y1 - 1997

N2 - Oxidative metabolic burst activity by heterophils from orange-winged Amazon parrots (Amazona amazonica amazonica) was indirectly evaluated by measuring the oxidation of non-fluorescent intracellular 2',7'-dichlorofluorescin (DCFH) to fluorescent 2',7/dichlorofluorescein (DCF) with flow cytometry. Heterophils were isolated from whole blood samples by preconcentrating the white blood cells with 3% hetastarch before running the samples through a polysucrose and disodium diatrizoate centrifugation. Phorbol myristate acetate (PMA) was used to activate the heterophils. Results show that heterophils from orange-winged Amazon parrots can oxidise DCFH to a fluorescent product and a wide range of DCF production was observed among individual birds. The production of DCF increased with PMA concentrations and significant differences were seen between the highest and lowest concentrations of PMA. These results support the concept that heterophils from orange-winged Amazon parrots can produce oxidative metabolites and that these heterophils appear to have functional heterogenicity.

AB - Oxidative metabolic burst activity by heterophils from orange-winged Amazon parrots (Amazona amazonica amazonica) was indirectly evaluated by measuring the oxidation of non-fluorescent intracellular 2',7'-dichlorofluorescin (DCFH) to fluorescent 2',7/dichlorofluorescein (DCF) with flow cytometry. Heterophils were isolated from whole blood samples by preconcentrating the white blood cells with 3% hetastarch before running the samples through a polysucrose and disodium diatrizoate centrifugation. Phorbol myristate acetate (PMA) was used to activate the heterophils. Results show that heterophils from orange-winged Amazon parrots can oxidise DCFH to a fluorescent product and a wide range of DCF production was observed among individual birds. The production of DCF increased with PMA concentrations and significant differences were seen between the highest and lowest concentrations of PMA. These results support the concept that heterophils from orange-winged Amazon parrots can produce oxidative metabolites and that these heterophils appear to have functional heterogenicity.

KW - 2',7'-Dichlorofluorescein (DCF)

KW - 2',7'-Dichlorofluorescin (DCFH)

KW - Flow cytometry

KW - Heterophil

KW - Orange-winged Amazon parrot

UR - http://www.scopus.com/inward/record.url?scp=0031468311&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031468311&partnerID=8YFLogxK

M3 - Article

VL - 7

SP - 197

EP - 201

JO - Comparative Clinical Pathology

JF - Comparative Clinical Pathology

SN - 1618-5641

IS - 4

ER -