Flow cytometric methods for the analysis of human basophil surface antigens and viability

Bruce S. Bochner, Alicia A. McKelvey, Robert P. Schleimer, James Hildreth, Donald W. MacGlashan

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

Fluorescence and flow microfluorometric methods have been established for the detection and evaluation of IgE-bearing human leukocytes in various cell preparations including those where basophils are present at low percentages. Quantitative techniques for the determination of basophil purity, viability, and cell surface antigens including IgE are described. Use of these methods will facilitate the identification and phenotypic analysis of human IgE-bearing cells in a wide variety of biological fluids.

Original languageEnglish (US)
Pages (from-to)265-271
Number of pages7
JournalJournal of Immunological Methods
Volume125
Issue number1-2
DOIs
StatePublished - Dec 20 1989
Externally publishedYes

Keywords

  • Basophil
  • Flow cytometry
  • IgE
  • Immunofluorescence
  • Viability

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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