Flow Cytometric Evaluation of Canine Bone Marrow Differential Cell Counts

Douglas J. Weiss, Melissa Blauvelt, Jane E Sykes, David McClenahan

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Three flow cytometric techniques were evaluated for determination of differential cell counts on canine clinical bone marrow specimens. Techniques included staining bone manrow specimens with 2'T'-dichlorofluorescein (DCF) or 3,3′-dihexyloxacarbocyanine iodide (DiOC6) and evaluation of forward-angle light scatter vs. side-angle light scatter plots. Flow cytometric evaluation of bone marrow cells stained with DCF failed to separate bone marrow cells into distinct cell populations. Staining with DiOC6 resulted in separation of bone marrow cells into populations of mature and immature erythroid cells, mature and immature myeloid cells, and lymphocytes. The scatter plot method resulted in identification of mature and immature erythroid cells, immature myeloid cells, metamyelocytes, and bands and segmenters. Lymphocytes could not be differentiated from mature erythroid cells by the scatter plot method. When the results of the DiOC6 method and the scatter plot method were compared with manual bone marrow differential cell counts, the scatter plot method had more similar mean values and higher correlation coefficients. The scatter plot method has the potential of providing rapid semiquantitative assessment of bone marrow differential cell counts in dogs for specimens that contain low numbers of lymphocytes.

Original languageEnglish (US)
Pages (from-to)97-104
Number of pages8
JournalVeterinary Clinical Pathology
Volume29
Issue number3
StatePublished - 2000
Externally publishedYes

Keywords

  • Bone marrow
  • Dog
  • Flow cytometry

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

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