Flow cytometric determination of genotoxic effects of exposure to petroleum in mink and sea otters

John W. Bickham, Jonna A Mazet, John Blake, Michael J. Smolen, Yigong Lou, Brenda E. Ballachey

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Three experiments were conducted to instigate the genotoxic effects of crude oil on mink and sea otters. In the first experiment, the effects on mink of chronic exposure to weathered Prudhoe Bay crude oil were studied. Female mink were fed a diet that included weathered crude oil for a period of 3 weeks prior to mating, during pregnancy and until weaning. Kits were exposed through lactation and by diet after weaning until 4 months of age. Kidney and liver tissues of the kits were examined using how cytometry (FCM) and it was found that the genome size was increased in kidney samples from the experimental group compared to the control group. This effect was probably due to some type of DNA amplification and it could have been inherited from the exposed mothers or have been a somatic response to oil exposure in the pups. No evidence of clastogenic effects, as measured by the coefficient of variation (CV) of the G1 peak, was found in kidney or liver tissue. In the second experiment, yearling female mink were exposed either by diet or externally to crude oil or bunker C fuel oil, Evidence for clastogenic damage was found in spleen tissue for the exposure groups, but not in kidney tissue. No evidence of increased genome size was observed. In the third experiment, blood was obtained from wild-caught sea otters in Prince William Sound. The sea otters represented two populations: one from western Prince William Sound that was potentially exposed to oil from the Exxon Valdez oil spill and a reference population from eastern Prince William Sound that did not receive oil from the spill. The spill had occurred 1.5 years prior to obtaining the blood samples. Although the mean CVs did not differ between the populations, the exposed population had a significantly higher variance of CV measurements and five out of 15 animals from the exposed population had CVs higher than the 95% confidence limits of the reference population. It is concluded that FCM is a sensitive indicator of the clastogenic effects of oil exposure and that haematopoietic tissues and blood are best for detecting clastogenic damage. Moreover, the observed differences in the genome size of the kidney cells were possibly heritable effects, but this needs further investigation. Lastly, sea otters exposed to spilled oil 1.5 years earlier showed evidence of clastogenic damage in one-third of the individuals sampled.

Original languageEnglish (US)
Pages (from-to)191-199
Number of pages9
JournalEcotoxicology
Volume7
Issue number4
DOIs
StatePublished - 1998

Fingerprint

Otters
Mink
Petroleum
Oils
petroleum
Petroleum Pollution
Tissue
Genome Size
Nutrition
crude oil
Kidney
Blood
oil
Genes
Population
Acoustic waves
Hazardous materials spills
genome
weaning
Liver

Keywords

  • Exxon Valdez
  • Flow cytometry
  • Genotoxicity
  • Mink
  • Oil
  • Sea otters

ASJC Scopus subject areas

  • Ecology
  • Environmental Science(all)
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Flow cytometric determination of genotoxic effects of exposure to petroleum in mink and sea otters. / Bickham, John W.; Mazet, Jonna A; Blake, John; Smolen, Michael J.; Lou, Yigong; Ballachey, Brenda E.

In: Ecotoxicology, Vol. 7, No. 4, 1998, p. 191-199.

Research output: Contribution to journalArticle

Bickham, John W. ; Mazet, Jonna A ; Blake, John ; Smolen, Michael J. ; Lou, Yigong ; Ballachey, Brenda E. / Flow cytometric determination of genotoxic effects of exposure to petroleum in mink and sea otters. In: Ecotoxicology. 1998 ; Vol. 7, No. 4. pp. 191-199.
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