Flow cytometric characterization and clinical outcome of CD4+ T-cell lymphoma in dogs

67 cases

P. R. Avery, Jenna H Burton, J. L. Bromberek, D. M. Seelig, R. Elmslie, S. Correa, E. J. Ehrhart, P. S. Morley, A. C. Avery

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: Canine T-cell lymphoma (TCL) is conventionally considered an aggressive disease, but some forms are histologically and clinically indolent. CD4 TCL is reported to be the most common subtype of TCL. We assessed flow cytometric characteristics, histologic features when available, and clinical outcomes of CD4+ TCL to determine if flow cytometry can be used to subclassify this group of lymphomas. Objective: To test the hypothesis that canine CD4+ T-cell lymphoma (TCL) is a homogeneous group of lymphomas with an aggressive clinical course. Animals: Sixty-seven dogs diagnosed with CD4+ TCL by flow cytometry and treated at 1 of 3 oncology referral clinics. Methods: Retrospective multivariable analysis of outcome in canine CD4+ TCL including patient characteristics, treatment, and flow cytometric features. Results: The majority of CD4+ TCL were CD45+, expressed low class II MHC, and exhibited an aggressive clinical course independent of treatment regimen (median survival, 159 days). Histologically, CD4+ TCL were classified as lymphoblastic or peripheral T cell. Size of the neoplastic lymphocytes had a modest effect on both PFI and survival in this group. A small number of CD4+ TCL were CD45- and class II MHC high, and exhibited an apparently more indolent clinical course (median survival not yet reached). Conclusions and Clinical Importance: Although the majority of CD4+ TCL in dogs had uniform clinical and flow cytometric features and an aggressive clinical course, a subset had a unique immunophenotype that predicts significantly longer survival. This finding strengthens the utility of flow cytometry to aid in the stratification of canine lymphoma.

Original languageEnglish (US)
Pages (from-to)538-546
Number of pages9
JournalJournal of Veterinary Internal Medicine
Volume28
Issue number2
DOIs
StatePublished - Mar 2014
Externally publishedYes

Fingerprint

T-Cell Lymphoma
lymphoma
T-lymphocytes
Dogs
dogs
Canidae
disease course
Lymphoma
Flow Cytometry
Survival
flow cytometry
Referral and Consultation
Lymphocytes
T-Lymphocytes

Keywords

  • Canine
  • Immunophenotyping
  • Lymphoblastic
  • Peripheral T cell
  • Prognosis

ASJC Scopus subject areas

  • veterinary(all)
  • Medicine(all)

Cite this

Flow cytometric characterization and clinical outcome of CD4+ T-cell lymphoma in dogs : 67 cases. / Avery, P. R.; Burton, Jenna H; Bromberek, J. L.; Seelig, D. M.; Elmslie, R.; Correa, S.; Ehrhart, E. J.; Morley, P. S.; Avery, A. C.

In: Journal of Veterinary Internal Medicine, Vol. 28, No. 2, 03.2014, p. 538-546.

Research output: Contribution to journalArticle

Avery, PR, Burton, JH, Bromberek, JL, Seelig, DM, Elmslie, R, Correa, S, Ehrhart, EJ, Morley, PS & Avery, AC 2014, 'Flow cytometric characterization and clinical outcome of CD4+ T-cell lymphoma in dogs: 67 cases', Journal of Veterinary Internal Medicine, vol. 28, no. 2, pp. 538-546. https://doi.org/10.1111/jvim.12304
Avery, P. R. ; Burton, Jenna H ; Bromberek, J. L. ; Seelig, D. M. ; Elmslie, R. ; Correa, S. ; Ehrhart, E. J. ; Morley, P. S. ; Avery, A. C. / Flow cytometric characterization and clinical outcome of CD4+ T-cell lymphoma in dogs : 67 cases. In: Journal of Veterinary Internal Medicine. 2014 ; Vol. 28, No. 2. pp. 538-546.
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AU - Seelig, D. M.

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AU - Ehrhart, E. J.

AU - Morley, P. S.

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