Flow cytometric analysis of testicular tissue: Detection of injury and recovery

Arline D. Deitch, Susan D. Goldberg, Ralph W deVere White

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

DNA flow cytometry was used to examine the effects of five chemotherapeutic agents, vinblastine, cisplatinum, 5-fluorouracil, cyclophosphamide and Adriamycin, on mouse testes. Alterations in the haploid (1C), diploid (2C) and tetraploid (4C) cell compartments were assessed at 11, 29 and 56 days after giving mice a single intraperitoneal injection of each agent. Analysis of the histograms proved to be a rapid and reproducible method of monitoring injury in this animal model. Because this procedure can detect subtle testicular injuries, it is predicted that DNA histograms obtained from fine needle aspirates will gain increasing acceptance in the management of the oligo- and azoospermic patient.

Original languageEnglish (US)
Pages (from-to)71-76
Number of pages6
JournalWorld Journal of Urology
Volume4
Issue number2
DOIs
StatePublished - Sep 1986

ASJC Scopus subject areas

  • Urology

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