Flow cytometric analysis of testicular tissue: Detection of injury and recovery

Arline D. Deitch, Susan D. Goldberg, Ralph W deVere White

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

DNA flow cytometry was used to examine the effects of five chemotherapeutic agents, vinblastine, cisplatinum, 5-fluorouracil, cyclophosphamide and Adriamycin, on mouse testes. Alterations in the haploid (1C), diploid (2C) and tetraploid (4C) cell compartments were assessed at 11, 29 and 56 days after giving mice a single intraperitoneal injection of each agent. Analysis of the histograms proved to be a rapid and reproducible method of monitoring injury in this animal model. Because this procedure can detect subtle testicular injuries, it is predicted that DNA histograms obtained from fine needle aspirates will gain increasing acceptance in the management of the oligo- and azoospermic patient.

Original languageEnglish (US)
Pages (from-to)71-76
Number of pages6
JournalWorld Journal of Urology
Volume4
Issue number2
DOIs
StatePublished - Sep 1986

Fingerprint

Tetraploidy
Vinblastine
Haploidy
DNA
Wounds and Injuries
Intraperitoneal Injections
Diploidy
Fluorouracil
Doxorubicin
Cyclophosphamide
Needles
Testis
Flow Cytometry
Animal Models

ASJC Scopus subject areas

  • Urology

Cite this

Flow cytometric analysis of testicular tissue : Detection of injury and recovery. / Deitch, Arline D.; Goldberg, Susan D.; deVere White, Ralph W.

In: World Journal of Urology, Vol. 4, No. 2, 09.1986, p. 71-76.

Research output: Contribution to journalArticle

@article{7bc5c460722842c0a261d826d5be3f7c,
title = "Flow cytometric analysis of testicular tissue: Detection of injury and recovery",
abstract = "DNA flow cytometry was used to examine the effects of five chemotherapeutic agents, vinblastine, cisplatinum, 5-fluorouracil, cyclophosphamide and Adriamycin, on mouse testes. Alterations in the haploid (1C), diploid (2C) and tetraploid (4C) cell compartments were assessed at 11, 29 and 56 days after giving mice a single intraperitoneal injection of each agent. Analysis of the histograms proved to be a rapid and reproducible method of monitoring injury in this animal model. Because this procedure can detect subtle testicular injuries, it is predicted that DNA histograms obtained from fine needle aspirates will gain increasing acceptance in the management of the oligo- and azoospermic patient.",
author = "Deitch, {Arline D.} and Goldberg, {Susan D.} and {deVere White}, {Ralph W}",
year = "1986",
month = "9",
doi = "10.1007/BF00326397",
language = "English (US)",
volume = "4",
pages = "71--76",
journal = "World Journal of Urology",
issn = "0724-4983",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Flow cytometric analysis of testicular tissue

T2 - Detection of injury and recovery

AU - Deitch, Arline D.

AU - Goldberg, Susan D.

AU - deVere White, Ralph W

PY - 1986/9

Y1 - 1986/9

N2 - DNA flow cytometry was used to examine the effects of five chemotherapeutic agents, vinblastine, cisplatinum, 5-fluorouracil, cyclophosphamide and Adriamycin, on mouse testes. Alterations in the haploid (1C), diploid (2C) and tetraploid (4C) cell compartments were assessed at 11, 29 and 56 days after giving mice a single intraperitoneal injection of each agent. Analysis of the histograms proved to be a rapid and reproducible method of monitoring injury in this animal model. Because this procedure can detect subtle testicular injuries, it is predicted that DNA histograms obtained from fine needle aspirates will gain increasing acceptance in the management of the oligo- and azoospermic patient.

AB - DNA flow cytometry was used to examine the effects of five chemotherapeutic agents, vinblastine, cisplatinum, 5-fluorouracil, cyclophosphamide and Adriamycin, on mouse testes. Alterations in the haploid (1C), diploid (2C) and tetraploid (4C) cell compartments were assessed at 11, 29 and 56 days after giving mice a single intraperitoneal injection of each agent. Analysis of the histograms proved to be a rapid and reproducible method of monitoring injury in this animal model. Because this procedure can detect subtle testicular injuries, it is predicted that DNA histograms obtained from fine needle aspirates will gain increasing acceptance in the management of the oligo- and azoospermic patient.

UR - http://www.scopus.com/inward/record.url?scp=0023018898&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023018898&partnerID=8YFLogxK

U2 - 10.1007/BF00326397

DO - 10.1007/BF00326397

M3 - Article

AN - SCOPUS:0023018898

VL - 4

SP - 71

EP - 76

JO - World Journal of Urology

JF - World Journal of Urology

SN - 0724-4983

IS - 2

ER -