FLIm and raman spectroscopy for investigating biochemical changes of bovine pericardium upon genipin cross-linking

Tanveer Ahmed Shaik, Alba Alfonso-Garcia, Martin Richter, Florian Korinth, Christoph Krafft, Laura Marcu, Jürgen Popp

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Biomaterials used in tissue engineering and regenerative medicine applications benefit from longitudinal monitoring in a non-destructive manner. Label-free imaging based on fluorescence lifetime imaging (FLIm) and Raman spectroscopy were used to monitor the degree of genipin (GE) cross-linking of antigen-removed bovine pericardium (ARBP) at three incubation time points (0.5, 1.0, and 2.5 h). Fluorescence lifetime decreased and the emission spectrum redshifted compared to that of uncross-linked ARBP. The Raman signature of GE-ARBP was resonance-enhanced due to the GE cross-linker that generated new Raman bands at 1165, 1326, 1350, 1380, 1402, 1470, 1506, 1535, 1574, 1630, 1728, and 1741 cm−1. These were validated through density functional theory calculations as cross-linker-specific bands. A multivariate multiple regression model was developed to enhance the biochemical specificity of FLIm parameters fluorescence intensity ratio (R2 = 0.92) and lifetime (R2 = 0.94)) with Raman spectral results. FLIm and Raman spectroscopy detected biochemical changes occurring in the collagenous tissue during the cross-linking process that were characterized by the formation of a blue pigment which affected the tissue fluorescence and scattering properties. In conclusion, FLIm parameters and Raman spectroscopy were used to monitor the degree of cross-linking non-destructively.

Original languageEnglish (US)
Article number25173857
JournalMolecules
Volume25
Issue number17
DOIs
StatePublished - Sep 2020

Keywords

  • Cross-linking
  • FLIm
  • Genipin
  • Raman spectroscopy
  • Tissue engineering

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'FLIm and raman spectroscopy for investigating biochemical changes of bovine pericardium upon genipin cross-linking'. Together they form a unique fingerprint.

Cite this