Fixation method useful for cytologic examination and dna flow cytometry of exfoliated bladder cells

Lydia P Howell, Arline D. Deitch, Vicki A. Andreotti, Lorri A. Westrick, Ralph W deVere White

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Single-institution studies have shown that DNA flow cytometry is superior to routine cytologic evaluation of following patients for bladder cancer recurrence. For 15 urine and 15 bladder washing specimens, we evaluated a fixative employing methanol plus acetic acid (MA), freshly mixed 20:1 (vol/vol). Routine cytologic evaluation following Papanicolaou staining, and DNA flow cytometry were performed. Paired aliquots from the same washings and urines were processed as fresh spray fixed samples and MA-fixed samples. The majority of the MA-fixed specimens showed good nuclear preservation when assessed for chromatin texture, presence of distinct nuclear envelope, and clarity of nucleolus, while only a minority of the fresh urine and washing samples showed these features. Cytoplasmic degeneration was seen only in fresh specimens. The presence of aneuploidy and the percentage of hyperdiploid cells could be reliably determined in the MA-fixed samples. This fixation protocol is recommended for the transport of urine and bladder washing specimens to centralized laboratories for both cytologic and flow cytometric evaluation.

Original languageEnglish (US)
Pages (from-to)472-475
Number of pages4
JournalUrology
Volume41
Issue number5
DOIs
StatePublished - 1993

Fingerprint

Flow Cytometry
Urinary Bladder
Urine
Fixatives
Polyploidy
DNA
Nuclear Envelope
Aneuploidy
Urinary Bladder Neoplasms
Acetic Acid
Chromatin
Methanol
Staining and Labeling
Recurrence

ASJC Scopus subject areas

  • Urology

Cite this

Fixation method useful for cytologic examination and dna flow cytometry of exfoliated bladder cells. / Howell, Lydia P; Deitch, Arline D.; Andreotti, Vicki A.; Westrick, Lorri A.; deVere White, Ralph W.

In: Urology, Vol. 41, No. 5, 1993, p. 472-475.

Research output: Contribution to journalArticle

Howell, Lydia P ; Deitch, Arline D. ; Andreotti, Vicki A. ; Westrick, Lorri A. ; deVere White, Ralph W. / Fixation method useful for cytologic examination and dna flow cytometry of exfoliated bladder cells. In: Urology. 1993 ; Vol. 41, No. 5. pp. 472-475.
@article{caa5da5ed6474c7dbb0dae2bb74e837b,
title = "Fixation method useful for cytologic examination and dna flow cytometry of exfoliated bladder cells",
abstract = "Single-institution studies have shown that DNA flow cytometry is superior to routine cytologic evaluation of following patients for bladder cancer recurrence. For 15 urine and 15 bladder washing specimens, we evaluated a fixative employing methanol plus acetic acid (MA), freshly mixed 20:1 (vol/vol). Routine cytologic evaluation following Papanicolaou staining, and DNA flow cytometry were performed. Paired aliquots from the same washings and urines were processed as fresh spray fixed samples and MA-fixed samples. The majority of the MA-fixed specimens showed good nuclear preservation when assessed for chromatin texture, presence of distinct nuclear envelope, and clarity of nucleolus, while only a minority of the fresh urine and washing samples showed these features. Cytoplasmic degeneration was seen only in fresh specimens. The presence of aneuploidy and the percentage of hyperdiploid cells could be reliably determined in the MA-fixed samples. This fixation protocol is recommended for the transport of urine and bladder washing specimens to centralized laboratories for both cytologic and flow cytometric evaluation.",
author = "Howell, {Lydia P} and Deitch, {Arline D.} and Andreotti, {Vicki A.} and Westrick, {Lorri A.} and {deVere White}, {Ralph W}",
year = "1993",
doi = "10.1016/0090-4295(93)90512-9",
language = "English (US)",
volume = "41",
pages = "472--475",
journal = "Urology",
issn = "1527-9995",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Fixation method useful for cytologic examination and dna flow cytometry of exfoliated bladder cells

AU - Howell, Lydia P

AU - Deitch, Arline D.

AU - Andreotti, Vicki A.

AU - Westrick, Lorri A.

AU - deVere White, Ralph W

PY - 1993

Y1 - 1993

N2 - Single-institution studies have shown that DNA flow cytometry is superior to routine cytologic evaluation of following patients for bladder cancer recurrence. For 15 urine and 15 bladder washing specimens, we evaluated a fixative employing methanol plus acetic acid (MA), freshly mixed 20:1 (vol/vol). Routine cytologic evaluation following Papanicolaou staining, and DNA flow cytometry were performed. Paired aliquots from the same washings and urines were processed as fresh spray fixed samples and MA-fixed samples. The majority of the MA-fixed specimens showed good nuclear preservation when assessed for chromatin texture, presence of distinct nuclear envelope, and clarity of nucleolus, while only a minority of the fresh urine and washing samples showed these features. Cytoplasmic degeneration was seen only in fresh specimens. The presence of aneuploidy and the percentage of hyperdiploid cells could be reliably determined in the MA-fixed samples. This fixation protocol is recommended for the transport of urine and bladder washing specimens to centralized laboratories for both cytologic and flow cytometric evaluation.

AB - Single-institution studies have shown that DNA flow cytometry is superior to routine cytologic evaluation of following patients for bladder cancer recurrence. For 15 urine and 15 bladder washing specimens, we evaluated a fixative employing methanol plus acetic acid (MA), freshly mixed 20:1 (vol/vol). Routine cytologic evaluation following Papanicolaou staining, and DNA flow cytometry were performed. Paired aliquots from the same washings and urines were processed as fresh spray fixed samples and MA-fixed samples. The majority of the MA-fixed specimens showed good nuclear preservation when assessed for chromatin texture, presence of distinct nuclear envelope, and clarity of nucleolus, while only a minority of the fresh urine and washing samples showed these features. Cytoplasmic degeneration was seen only in fresh specimens. The presence of aneuploidy and the percentage of hyperdiploid cells could be reliably determined in the MA-fixed samples. This fixation protocol is recommended for the transport of urine and bladder washing specimens to centralized laboratories for both cytologic and flow cytometric evaluation.

UR - http://www.scopus.com/inward/record.url?scp=0027315933&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027315933&partnerID=8YFLogxK

U2 - 10.1016/0090-4295(93)90512-9

DO - 10.1016/0090-4295(93)90512-9

M3 - Article

C2 - 7683835

AN - SCOPUS:0027315933

VL - 41

SP - 472

EP - 475

JO - Urology

JF - Urology

SN - 1527-9995

IS - 5

ER -