Fission yeast TOR complex 2 activates the AGC-family Gad8 kinase essential for stress resistance and cell cycle control

Kyoko Ikeda, Susumu Morigasaki, Hisashi Tatebe, Fuyuhiko Tamanoi, Kazuhiro Shiozaki

Research output: Contribution to journalArticlepeer-review

64 Scopus citations


Members of the mitogen-activated protein kinase (MAPK) subfamily responsive to environmental stress stimuli are known as SAPKs (stress-activated protein kinases), which are conserved from yeast to humans. In the fission yeast Schizosaccharomyces pombe, Spc1/Sty1 SAPK is activated by diverse forms of stress, such as osmostress, oxidative stress and heat shock, and induces gene expression through the Atf1 transcription factor. Sin1 (SAPK interacting protein 1) was originally isolated as a protein that interacts with Spc1, and its orthologs were also found in diverse eukaryotes. Here we report that Sin1 is not required for the stress gene expression regulated by Spc1 and Atf1, and that Sin1 is an essential component of TOR (target of rapamycin) complex 2 (TORC2). TORC2 is not essential for cell viability in S. pombe but plays important roles in cellular survival of stress conditions through phosphorylation and activation of an AGC-family protein kinase, Gad8. In addition, inactivation of Gad8 results in a synthetic growth defect with cdc25-22, a temperature-sensitive mutation of the Cdc25 phosphatase that activates Cdc2 kinase at G2/M. Gad8 also positively regulates expression of the CDK inhibitor gene rum1 +, which is essential for cell cycle arrest in G1 after nitrogen starvation. These results strongly suggest that the TORC2-Gad8 pathway has multiple physiological functions in cellular stress resistance and cell cycle progression at both G1/S and G2/M transitions.

Original languageEnglish (US)
Pages (from-to)358-364
Number of pages7
JournalCell Cycle
Issue number3
StatePublished - Feb 1 2008


  • Cell cycle
  • Fission yeast
  • Stress
  • TOR
  • TORC2

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry
  • Molecular Biology


Dive into the research topics of 'Fission yeast TOR complex 2 activates the AGC-family Gad8 kinase essential for stress resistance and cell cycle control'. Together they form a unique fingerprint.

Cite this