First report of Spring Viremia of Carp Virus (SVCV) in wild common carp in North America

Audrey L. Dikkeboom, Craig Radi, Kathy L. Kurth, Susan Marcquenski, Marty Engel, Andrew E. Goodwin, Keith Way, David M. Stone, Clare Longshaw

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

In spring 2002, an estimated 1,500 common carp Cyprinus carpio in Cedar Lake, northwestern Wisconsin, died over a 6-week period from late April through the first week in June. Three moribund carp were necropsied and had signs consistent with spring viremia of carp (SVC) disease, including petechiae and ecchymotic hemorrhages on the skin, ascites, and edematous kidney and spleen. A virus was isolated on fathead minnow cells and shown to be a rhabdovirus by electron microscopy. Immunoassay results indicated a close serological relationship with spring viremia of carp virus (SVCV). This was confirmed by a reverse transcriptase-polymerase chain reaction assay and subsequent analysis of a subsection glycoprotein gene. Immunocytochemistry and serum neutralization tests indicated that the Cedar Lake isolate did not share complete antigenic identity with the European reference SVCV Also, the isolate showed an inhibition of cytopathic effect after repeated subculture in epithelioma papulosum cyprini and bluegill fibroblast cells when compared with other SVCV isolates. In virus transmission studies the isolate was shown to be of low virulence for juvenile carp. Sequence analysis showed that the Cedar Lake SVCV isolate was more closely related to a recently isolated North Carolina strain of SVCV (98.6% nucleotide identity) and to strains of Asian origin rather than the European reference strain of SVCV. This is the first report of an SVC epizootic in a wild common carp population in North America.

Original languageEnglish (US)
Pages (from-to)169-178
Number of pages10
JournalJournal of Aquatic Animal Health
Volume16
Issue number4
DOIs
StatePublished - Dec 1 2004
Externally publishedYes

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Spring viraemia of carp virus
Carps
Viremia
North America
Cyprinus carpio
virus
Viruses
spring viremia of carp
carp
lakes
Papulosa
Lakes
Rhabdoviridae
Pimephales promelas
lake
Lepomis macrochirus
cytopathogenicity
ascites
virus transmission
immunocytochemistry

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary(all)

Cite this

Dikkeboom, A. L., Radi, C., Kurth, K. L., Marcquenski, S., Engel, M., Goodwin, A. E., ... Longshaw, C. (2004). First report of Spring Viremia of Carp Virus (SVCV) in wild common carp in North America. Journal of Aquatic Animal Health, 16(4), 169-178. https://doi.org/10.1577/H03-064.1

First report of Spring Viremia of Carp Virus (SVCV) in wild common carp in North America. / Dikkeboom, Audrey L.; Radi, Craig; Kurth, Kathy L.; Marcquenski, Susan; Engel, Marty; Goodwin, Andrew E.; Way, Keith; Stone, David M.; Longshaw, Clare.

In: Journal of Aquatic Animal Health, Vol. 16, No. 4, 01.12.2004, p. 169-178.

Research output: Contribution to journalArticle

Dikkeboom, AL, Radi, C, Kurth, KL, Marcquenski, S, Engel, M, Goodwin, AE, Way, K, Stone, DM & Longshaw, C 2004, 'First report of Spring Viremia of Carp Virus (SVCV) in wild common carp in North America', Journal of Aquatic Animal Health, vol. 16, no. 4, pp. 169-178. https://doi.org/10.1577/H03-064.1
Dikkeboom, Audrey L. ; Radi, Craig ; Kurth, Kathy L. ; Marcquenski, Susan ; Engel, Marty ; Goodwin, Andrew E. ; Way, Keith ; Stone, David M. ; Longshaw, Clare. / First report of Spring Viremia of Carp Virus (SVCV) in wild common carp in North America. In: Journal of Aquatic Animal Health. 2004 ; Vol. 16, No. 4. pp. 169-178.
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