First detection of Erysipelothrix sp. Infection in western mosquitofish Gambusia affinis inhabiting catfish aquaculture ponds in Mississippi, USA

Justin M. Stilwell, Matt J. Griffin, Thomas Graham Rosser, John H. Leary, Katharina Hagen-Frei, Charles C. Mischke, Eric K. Pomaranski, Esteban Soto Martinez, Alvin C. Camus

Research output: Contribution to journalArticle

Abstract

Native and introduced fish can serve as reservoirs for pathogens of cultured fish species. In the current study, 351 archived western mosquitofish Gambusia affinis collected from experimental catfish production ponds in Mississippi, USA, were surveyed histologically to evaluate their potential as vectors for fish pathogens. In addition to epitheliocystis and multiple meta-zoan parasites, 8 fish had widespread basophilic colonies of small Gram-positive rods associated primarily with stroma supporting the skeletal muscle and bone, as well as connective tissue components of other tissues and organ systems, such as perivascular adventitia and basement membranes. These findings were consistent with spaC-type Erysipelothrix sp. infections in ornamental fish cultured in the USA. The 16S rRNA, gyrase B (gyrB), and surface protective antigen (spa) genes were amplified and sequenced from bacterial colonies excised from paraffin-embedded tissue sections using laser capture microdissection. Molecular data confirmed the identity of a spaC-type Erysipelothrix sp., which grouped phylogenetically with spaC-type Erysipelothrix sp. from diseased ornamental fish. Given the significance of commercial catfish aquaculture in the southeastern USA and the widespread distribution of mosquitofish in catfish ponds throughout the region, infectivity trials with channel catfish Ictalurus punctatus were conducted. Catfish fingerlings were exposed to a spaC-type Erysipelothrix sp. isolate by intracoelomic injection and gavage. No mortality was observed in catfish exposed by either route, and surviving fish demonstrated no significant histopathologic lesions, suggesting channel catfish have low susceptibility to the bacteria. Further research is warranted to investigate the susceptibility of other cultured fish species to this emergent fish pathogen.

Original languageEnglish (US)
Pages (from-to)39-46
Number of pages8
JournalDiseases of Aquatic Organisms
Volume133
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

Erysipelothrix
Gambusia affinis
catfish
aquaculture
pond
fish
infection
Ictalurus punctatus
ornamental fish
pathogen
pathogens
Gambusia
basement membrane
fingerlings
detection
lesions (animal)
connective tissues
alkanes
lasers
infectivity

Keywords

  • Channel catfish
  • Erysipelothrix
  • Laser capture microdissection
  • Mosquitofish

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Cite this

Stilwell, J. M., Griffin, M. J., Rosser, T. G., Leary, J. H., Hagen-Frei, K., Mischke, C. C., ... Camus, A. C. (2019). First detection of Erysipelothrix sp. Infection in western mosquitofish Gambusia affinis inhabiting catfish aquaculture ponds in Mississippi, USA. Diseases of Aquatic Organisms, 133(1), 39-46. https://doi.org/10.3354/dao03332

First detection of Erysipelothrix sp. Infection in western mosquitofish Gambusia affinis inhabiting catfish aquaculture ponds in Mississippi, USA. / Stilwell, Justin M.; Griffin, Matt J.; Rosser, Thomas Graham; Leary, John H.; Hagen-Frei, Katharina; Mischke, Charles C.; Pomaranski, Eric K.; Soto Martinez, Esteban; Camus, Alvin C.

In: Diseases of Aquatic Organisms, Vol. 133, No. 1, 01.01.2019, p. 39-46.

Research output: Contribution to journalArticle

Stilwell, Justin M. ; Griffin, Matt J. ; Rosser, Thomas Graham ; Leary, John H. ; Hagen-Frei, Katharina ; Mischke, Charles C. ; Pomaranski, Eric K. ; Soto Martinez, Esteban ; Camus, Alvin C. / First detection of Erysipelothrix sp. Infection in western mosquitofish Gambusia affinis inhabiting catfish aquaculture ponds in Mississippi, USA. In: Diseases of Aquatic Organisms. 2019 ; Vol. 133, No. 1. pp. 39-46.
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abstract = "Native and introduced fish can serve as reservoirs for pathogens of cultured fish species. In the current study, 351 archived western mosquitofish Gambusia affinis collected from experimental catfish production ponds in Mississippi, USA, were surveyed histologically to evaluate their potential as vectors for fish pathogens. In addition to epitheliocystis and multiple meta-zoan parasites, 8 fish had widespread basophilic colonies of small Gram-positive rods associated primarily with stroma supporting the skeletal muscle and bone, as well as connective tissue components of other tissues and organ systems, such as perivascular adventitia and basement membranes. These findings were consistent with spaC-type Erysipelothrix sp. infections in ornamental fish cultured in the USA. The 16S rRNA, gyrase B (gyrB), and surface protective antigen (spa) genes were amplified and sequenced from bacterial colonies excised from paraffin-embedded tissue sections using laser capture microdissection. Molecular data confirmed the identity of a spaC-type Erysipelothrix sp., which grouped phylogenetically with spaC-type Erysipelothrix sp. from diseased ornamental fish. Given the significance of commercial catfish aquaculture in the southeastern USA and the widespread distribution of mosquitofish in catfish ponds throughout the region, infectivity trials with channel catfish Ictalurus punctatus were conducted. Catfish fingerlings were exposed to a spaC-type Erysipelothrix sp. isolate by intracoelomic injection and gavage. No mortality was observed in catfish exposed by either route, and surviving fish demonstrated no significant histopathologic lesions, suggesting channel catfish have low susceptibility to the bacteria. Further research is warranted to investigate the susceptibility of other cultured fish species to this emergent fish pathogen.",
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