Fibroblast growth factor-2 in remodeling of the developing basement membrane zone in the trachea of infant rhesus monkeys sensitized and challenged with allergen

Michael J. Evans, Laura S. Van Winkle, Michelle V. Fanucchi, Gregory L. Baker, April E. Murphy, Susan J. Nishio, Edward S Schelegle, Laurel J Gershwin, Philip L. Sannes, Charles Plopper

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Abstract

Remodeling of the epithelial basement membrane zone (BMZ) involves increased deposition of collagen, resulting in thickening of the BMZ. The current study focuses on fibroblast growth factor-2 (FGF-2) in the tracheal BMZ in house dust mite allergen (HDMA)-sensitized infant rhesus monkeys. challenged with HDMA at a time when the BMZ is undergoing active postnatal development. To detect structural changes in the BMZ, we measured collagens I, III, and V. To detect changes in the function of the BMZ, we measured immunoreactivity of the heparan sulfate proteoglycan, perlecan, and FGF-2. We found significant thickening of the tracheal BMZ (p < 0.05) with each of these parameters. We also found that all HDMA tracheal samples expressed thin focal areas of the BMZ associated with leukocyte trafficking. These areas were depleted of perlecan and FGF-2; however, increased FGF-2 immunoreactivity was present in the adjacent basal cells. We conclude that basal cells and FGF-2 are involved with significant remodeling of the BMZ in the developing trachea of infant rhesus monkeys exposed to HDMA.

Original languageEnglish (US)
Pages (from-to)1747-1754
Number of pages8
JournalLaboratory Investigation
Volume82
Issue number12
StatePublished - Dec 1 2002

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Fibroblast Growth Factor 2
Trachea
Macaca mulatta
Basement Membrane
Allergens
Dermatophagoides Antigens
Collagen
Heparan Sulfate Proteoglycans
Leukocytes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Fibroblast growth factor-2 in remodeling of the developing basement membrane zone in the trachea of infant rhesus monkeys sensitized and challenged with allergen. / Evans, Michael J.; Van Winkle, Laura S.; Fanucchi, Michelle V.; Baker, Gregory L.; Murphy, April E.; Nishio, Susan J.; Schelegle, Edward S; Gershwin, Laurel J; Sannes, Philip L.; Plopper, Charles.

In: Laboratory Investigation, Vol. 82, No. 12, 01.12.2002, p. 1747-1754.

Research output: Contribution to journalArticle

Evans, Michael J. ; Van Winkle, Laura S. ; Fanucchi, Michelle V. ; Baker, Gregory L. ; Murphy, April E. ; Nishio, Susan J. ; Schelegle, Edward S ; Gershwin, Laurel J ; Sannes, Philip L. ; Plopper, Charles. / Fibroblast growth factor-2 in remodeling of the developing basement membrane zone in the trachea of infant rhesus monkeys sensitized and challenged with allergen. In: Laboratory Investigation. 2002 ; Vol. 82, No. 12. pp. 1747-1754.
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