Fibroblast growth factor-2 during postnatal development of the tracheal basement membrane zone

Michael J. Evans, Michelle V. Fanucchi, Laura S. Van Winkle, Gregory L. Baker, April E. Murphy, Susan J. Nishio, Philip L. Sannes, Charles Plopper

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Thickening of the basement membrane zone (BMZ) is a characteristic of several airway diseases; however, very little is known about how this process occurs. The purpose of this study was to define development of the BMZ in the trachea of growing rhesus monkeys at 1, 2, 3, and 6 mo of age. We measured immunoreactivity of collagen types I, III, and V to detect structural changes in the developing BMZ. To detect more dynamic, functional components of the epithelial-mesenchymal trophic unit, we evaluated the distribution of perlecan, fibroblast growth factor-2 (FGF-2), and fibroblast growth factor receptor-1 (FGFR-1). One-month-old monkeys had a mean collagen BMZ width of 1.5 ± 0.7 μm that increased to 4.4 ± 0.4 μm in 6-mo-old monkeys. Perlecan was localized in the BMZ of the epithelium at all ages. FGF-2 was strongly expressed in basal cells at 1-3 mo. At 6 mo, FGF-2 was expressed throughout the BMZ and weakly in basal cells. FGFR-1 immunoreactivity was expressed by basal cells and cilia and weakly in the nuclei of columnar cells at all time points. These data indicate that development of the BMZ is a postnatal event in the rhesus monkey that involves FGF-2.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume283
Issue number6 27-6
StatePublished - Dec 1 2002

Fingerprint

Fibroblast Growth Factor 2
Basement Membrane
Receptor, Fibroblast Growth Factor, Type 1
Macaca mulatta
Haplorhini
Collagen Type V
Collagen Type III
Cilia
Collagen Type I
Trachea
Cell Nucleus
Collagen
Epithelium

Keywords

  • Basal cell
  • Fibroblast growth factor receptor-1
  • Perlecan
  • Rhesus monkey

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology

Cite this

Fibroblast growth factor-2 during postnatal development of the tracheal basement membrane zone. / Evans, Michael J.; Fanucchi, Michelle V.; Van Winkle, Laura S.; Baker, Gregory L.; Murphy, April E.; Nishio, Susan J.; Sannes, Philip L.; Plopper, Charles.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 283, No. 6 27-6, 01.12.2002.

Research output: Contribution to journalArticle

Evans, MJ, Fanucchi, MV, Van Winkle, LS, Baker, GL, Murphy, AE, Nishio, SJ, Sannes, PL & Plopper, C 2002, 'Fibroblast growth factor-2 during postnatal development of the tracheal basement membrane zone', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 283, no. 6 27-6.
Evans, Michael J. ; Fanucchi, Michelle V. ; Van Winkle, Laura S. ; Baker, Gregory L. ; Murphy, April E. ; Nishio, Susan J. ; Sannes, Philip L. ; Plopper, Charles. / Fibroblast growth factor-2 during postnatal development of the tracheal basement membrane zone. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2002 ; Vol. 283, No. 6 27-6.
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