Fiber-based platform for synchronous imaging of endogenous and exogenous fluorescence of biological tissue

Alba Alfonso-Garcia, Cai Li, Julien Bec, Diego Yankelevich, Laura Marcu, Ben Sherlock

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Endogenous and exogenous fluorescence emission from biological samples encodes complementary information. Here we report, to the best of our knowledge, the first results from an optical imaging platform with interleaved excitation and detection of exogenous and endogenous fluorescence from tissue samples using a single flexible multimode fiber that delivers the excitation beam and collects the emitted light. A custom-built reflective optical chopper wheel with synchronized rotation temporally multiplexes an autofluorescence lifetime imaging apparatus with an intensity-based fluorescence module tailored to imaging green fluorescent protein. We demonstrate the functionality of such platform imaging dyes of varying fluorescence signatures and resolving cellularized areas on bio-engineered tissue constructs.

Original languageEnglish (US)
Pages (from-to)3350-3353
Number of pages4
JournalOptics Letters
Volume44
Issue number13
DOIs
StatePublished - Jan 1 2019

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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    Alfonso-Garcia, A., Li, C., Bec, J., Yankelevich, D., Marcu, L., & Sherlock, B. (2019). Fiber-based platform for synchronous imaging of endogenous and exogenous fluorescence of biological tissue. Optics Letters, 44(13), 3350-3353. https://doi.org/10.1364/OL.44.003350