Fiber-based fluorescence lifetime imaging of recellularization processes on vascular tissue constructs

Alba Alfonso-Garcia, Jeny Shklover, Benjamin E. Sherlock, Alyssa Panitch, Leigh G. Griffiths, Laura Marcu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

New techniques able to monitor the maturation of tissue engineered constructs over time are needed for a more efficient control of developmental parameters. Here, a label-free fluorescence lifetime imaging (FLIm) approach implemented through a single fiber-optic interface is reported for nondestructive in situ assessment of vascular biomaterials. Recellularization processes of antigen removed bovine pericardium scaffolds with endothelial cells and mesenchymal stem cells were evaluated on the serous and the fibrous sides of the scaffolds, 2 distinct extracellular matrix niches, over the course of a 7 day culture period. Results indicated that fluorescence lifetime successfully report cell presence resolved from extracellular matrix fluorescence. The recellularization process was more rapid on the serous side than on the fibrous side for both cell types, and endothelial cells expanded faster than mesenchymal stem cells on antigen-removed bovine pericardium. Fiber-based FLIm has the potential to become a nondestructive tool for the assessment of tissue maturation by allowing in situ imaging of intraluminal vascular biomaterials.

Original languageEnglish (US)
Article numbere201700391
JournalJournal of Biophotonics
Volume11
Issue number9
DOIs
StatePublished - Sep 1 2018

Fingerprint

Pericardium
Optical Imaging
Biocompatible Materials
Mesenchymal Stromal Cells
Extracellular Matrix
Blood Vessels
Endothelial Cells
Fluorescence
Tissue
Imaging techniques
Antigens
life (durability)
fluorescence
fibers
Fibers
stem cells
Endothelial cells
antigens
Stem cells
Biomaterials

Keywords

  • fiber optics imaging
  • fluorescence lifetime imaging
  • tissue engineering

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Alfonso-Garcia, A., Shklover, J., Sherlock, B. E., Panitch, A., Griffiths, L. G., & Marcu, L. (2018). Fiber-based fluorescence lifetime imaging of recellularization processes on vascular tissue constructs. Journal of Biophotonics, 11(9), [e201700391]. https://doi.org/10.1002/jbio.201700391

Fiber-based fluorescence lifetime imaging of recellularization processes on vascular tissue constructs. / Alfonso-Garcia, Alba; Shklover, Jeny; Sherlock, Benjamin E.; Panitch, Alyssa; Griffiths, Leigh G.; Marcu, Laura.

In: Journal of Biophotonics, Vol. 11, No. 9, e201700391, 01.09.2018.

Research output: Contribution to journalArticle

Alfonso-Garcia, A, Shklover, J, Sherlock, BE, Panitch, A, Griffiths, LG & Marcu, L 2018, 'Fiber-based fluorescence lifetime imaging of recellularization processes on vascular tissue constructs', Journal of Biophotonics, vol. 11, no. 9, e201700391. https://doi.org/10.1002/jbio.201700391
Alfonso-Garcia A, Shklover J, Sherlock BE, Panitch A, Griffiths LG, Marcu L. Fiber-based fluorescence lifetime imaging of recellularization processes on vascular tissue constructs. Journal of Biophotonics. 2018 Sep 1;11(9). e201700391. https://doi.org/10.1002/jbio.201700391
Alfonso-Garcia, Alba ; Shklover, Jeny ; Sherlock, Benjamin E. ; Panitch, Alyssa ; Griffiths, Leigh G. ; Marcu, Laura. / Fiber-based fluorescence lifetime imaging of recellularization processes on vascular tissue constructs. In: Journal of Biophotonics. 2018 ; Vol. 11, No. 9.
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