Abstract
Porous, two-ply tubular chitosan conduits for guided tissue regeneration were fabricated by combining the textile technique (inner layer) with the thermally induced phase separation process (outer layer). A hollow chitosan tube was prepared using an industrial warp knitting process with chitosan yarns. Then, an appropriate diameter mandrel was inserted into the pre-fabricated tube. The tube and the mandrel were dipped into the chitosan solution together, taken out, and freeze-dried. After being neutralized in alkaline solution and dried at room temperature, the mandrel was removed to create the chitosan tubular scaffold. Scanning electron micrographs show that the resulting tubes have a biphasic wall structure, with a fibrous inner layer and a semipermeable outer layer. The swelling properties and the mechanical strength before and after in vitro degradation were investigated. The biocompatibility of the scaffolds was also investigated by co-culturing neuroblastoma cells (N2A, mouse) with the scaffolds. The results suggest that these chitosan tubular scaffolds are useful for the regeneration of tissues requiring a tubular scaffold.
Original language | English (US) |
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Pages (from-to) | 449-453 |
Number of pages | 5 |
Journal | Tsinghua Science and Technology |
Volume | 10 |
Issue number | 4 |
DOIs | |
State | Published - Aug 2005 |
Externally published | Yes |
Keywords
- Chitosan
- Knitting
- Nerve conduit
- Tissue engineering
ASJC Scopus subject areas
- General