Fiber-based chitosan tubular scaffolds for soft tissue engineering: Fabrication and in vitro evaluation

Aijun Wang, Qiang Ao, Wenling Cao, Chang Zhao, Yandao Gong, Nanming Zhao, Xiufang Zhang

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Porous, two-ply tubular chitosan conduits for guided tissue regeneration were fabricated by combining the textile technique (inner layer) with the thermally induced phase separation process (outer layer). A hollow chitosan tube was prepared using an industrial warp knitting process with chitosan yarns. Then, an appropriate diameter mandrel was inserted into the pre-fabricated tube. The tube and the mandrel were dipped into the chitosan solution together, taken out, and freeze-dried. After being neutralized in alkaline solution and dried at room temperature, the mandrel was removed to create the chitosan tubular scaffold. Scanning electron micrographs show that the resulting tubes have a biphasic wall structure, with a fibrous inner layer and a semipermeable outer layer. The swelling properties and the mechanical strength before and after in vitro degradation were investigated. The biocompatibility of the scaffolds was also investigated by co-culturing neuroblastoma cells (N2A, mouse) with the scaffolds. The results suggest that these chitosan tubular scaffolds are useful for the regeneration of tissues requiring a tubular scaffold.

Original languageEnglish (US)
Pages (from-to)449-453
Number of pages5
JournalTsinghua Science and Technology
Volume10
Issue number4
DOIs
StatePublished - Aug 2005
Externally publishedYes

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Keywords

  • Chitosan
  • Knitting
  • Nerve conduit
  • Tissue engineering

ASJC Scopus subject areas

  • General

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