Abstract
Porous, two-ply tubular chitosan conduits for guided tissue regeneration were fabricated by combining the textile technique (inner layer) with the thermally induced phase separation process (outer layer). A hollow chitosan tube was prepared using an industrial warp knitting process with chitosan yarns. Then, an appropriate diameter mandrel was inserted into the pre-fabricated tube. The tube and the mandrel were dipped into the chitosan solution together, taken out, and freeze-dried. After being neutralized in alkaline solution and dried at room temperature, the mandrel was removed to create the chitosan tubular scaffold. Scanning electron micrographs show that the resulting tubes have a biphasic wall structure, with a fibrous inner layer and a semipermeable outer layer. The swelling properties and the mechanical strength before and after in vitro degradation were investigated. The biocompatibility of the scaffolds was also investigated by co-culturing neuroblastoma cells (N2A, mouse) with the scaffolds. The results suggest that these chitosan tubular scaffolds are useful for the regeneration of tissues requiring a tubular scaffold.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 449-453 |
| Number of pages | 5 |
| Journal | Tsinghua Science and Technology |
| Volume | 10 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 2005 |
| Externally published | Yes |
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Keywords
- Chitosan
- Knitting
- Nerve conduit
- Tissue engineering
ASJC Scopus subject areas
- General
Cite this
Fiber-based chitosan tubular scaffolds for soft tissue engineering : Fabrication and in vitro evaluation. / Wang, Aijun; Ao, Qiang; Cao, Wenling; Zhao, Chang; Gong, Yandao; Zhao, Nanming; Zhang, Xiufang.
In: Tsinghua Science and Technology, Vol. 10, No. 4, 08.2005, p. 449-453.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fiber-based chitosan tubular scaffolds for soft tissue engineering
T2 - Fabrication and in vitro evaluation
AU - Wang, Aijun
AU - Ao, Qiang
AU - Cao, Wenling
AU - Zhao, Chang
AU - Gong, Yandao
AU - Zhao, Nanming
AU - Zhang, Xiufang
PY - 2005/8
Y1 - 2005/8
N2 - Porous, two-ply tubular chitosan conduits for guided tissue regeneration were fabricated by combining the textile technique (inner layer) with the thermally induced phase separation process (outer layer). A hollow chitosan tube was prepared using an industrial warp knitting process with chitosan yarns. Then, an appropriate diameter mandrel was inserted into the pre-fabricated tube. The tube and the mandrel were dipped into the chitosan solution together, taken out, and freeze-dried. After being neutralized in alkaline solution and dried at room temperature, the mandrel was removed to create the chitosan tubular scaffold. Scanning electron micrographs show that the resulting tubes have a biphasic wall structure, with a fibrous inner layer and a semipermeable outer layer. The swelling properties and the mechanical strength before and after in vitro degradation were investigated. The biocompatibility of the scaffolds was also investigated by co-culturing neuroblastoma cells (N2A, mouse) with the scaffolds. The results suggest that these chitosan tubular scaffolds are useful for the regeneration of tissues requiring a tubular scaffold.
AB - Porous, two-ply tubular chitosan conduits for guided tissue regeneration were fabricated by combining the textile technique (inner layer) with the thermally induced phase separation process (outer layer). A hollow chitosan tube was prepared using an industrial warp knitting process with chitosan yarns. Then, an appropriate diameter mandrel was inserted into the pre-fabricated tube. The tube and the mandrel were dipped into the chitosan solution together, taken out, and freeze-dried. After being neutralized in alkaline solution and dried at room temperature, the mandrel was removed to create the chitosan tubular scaffold. Scanning electron micrographs show that the resulting tubes have a biphasic wall structure, with a fibrous inner layer and a semipermeable outer layer. The swelling properties and the mechanical strength before and after in vitro degradation were investigated. The biocompatibility of the scaffolds was also investigated by co-culturing neuroblastoma cells (N2A, mouse) with the scaffolds. The results suggest that these chitosan tubular scaffolds are useful for the regeneration of tissues requiring a tubular scaffold.
KW - Chitosan
KW - Knitting
KW - Nerve conduit
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=24044487175&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=24044487175&partnerID=8YFLogxK
U2 - 10.1016/S1007-0214(05)70099-7
DO - 10.1016/S1007-0214(05)70099-7
M3 - Article
AN - SCOPUS:24044487175
VL - 10
SP - 449
EP - 453
JO - Tsinghua Science and Technology
JF - Tsinghua Science and Technology
SN - 1007-0214
IS - 4
ER -