Fiber-based chitosan tubular scaffolds for soft tissue engineering: Fabrication and in vitro evaluation

Aijun Wang, Qiang Ao, Wenling Cao, Chang Zhao, Yandao Gong, Nanming Zhao, Xiufang Zhang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Porous, two-ply tubular chitosan conduits for guided tissue regeneration were fabricated by combining the textile technique (inner layer) with the thermally induced phase separation process (outer layer). A hollow chitosan tube was prepared using an industrial warp knitting process with chitosan yarns. Then, an appropriate diameter mandrel was inserted into the pre-fabricated tube. The tube and the mandrel were dipped into the chitosan solution together, taken out, and freeze-dried. After being neutralized in alkaline solution and dried at room temperature, the mandrel was removed to create the chitosan tubular scaffold. Scanning electron micrographs show that the resulting tubes have a biphasic wall structure, with a fibrous inner layer and a semipermeable outer layer. The swelling properties and the mechanical strength before and after in vitro degradation were investigated. The biocompatibility of the scaffolds was also investigated by co-culturing neuroblastoma cells (N2A, mouse) with the scaffolds. The results suggest that these chitosan tubular scaffolds are useful for the regeneration of tissues requiring a tubular scaffold.

Original languageEnglish (US)
Pages (from-to)449-453
Number of pages5
JournalTsinghua Science and Technology
Volume10
Issue number4
DOIs
StatePublished - Aug 2005
Externally publishedYes

Fingerprint

Chitosan
Scaffolds (biology)
Tissue engineering
Fabrication
Scaffolds
Fibers
Tissue regeneration
Biocompatibility
Phase separation
Strength of materials
Swelling
Yarn
Textiles
Tissue
Scanning
Degradation
Electrons

Keywords

  • Chitosan
  • Knitting
  • Nerve conduit
  • Tissue engineering

ASJC Scopus subject areas

  • General

Cite this

Fiber-based chitosan tubular scaffolds for soft tissue engineering : Fabrication and in vitro evaluation. / Wang, Aijun; Ao, Qiang; Cao, Wenling; Zhao, Chang; Gong, Yandao; Zhao, Nanming; Zhang, Xiufang.

In: Tsinghua Science and Technology, Vol. 10, No. 4, 08.2005, p. 449-453.

Research output: Contribution to journalArticle

Wang, Aijun ; Ao, Qiang ; Cao, Wenling ; Zhao, Chang ; Gong, Yandao ; Zhao, Nanming ; Zhang, Xiufang. / Fiber-based chitosan tubular scaffolds for soft tissue engineering : Fabrication and in vitro evaluation. In: Tsinghua Science and Technology. 2005 ; Vol. 10, No. 4. pp. 449-453.
@article{b85519127f2c483794d5be2847c3054a,
title = "Fiber-based chitosan tubular scaffolds for soft tissue engineering: Fabrication and in vitro evaluation",
abstract = "Porous, two-ply tubular chitosan conduits for guided tissue regeneration were fabricated by combining the textile technique (inner layer) with the thermally induced phase separation process (outer layer). A hollow chitosan tube was prepared using an industrial warp knitting process with chitosan yarns. Then, an appropriate diameter mandrel was inserted into the pre-fabricated tube. The tube and the mandrel were dipped into the chitosan solution together, taken out, and freeze-dried. After being neutralized in alkaline solution and dried at room temperature, the mandrel was removed to create the chitosan tubular scaffold. Scanning electron micrographs show that the resulting tubes have a biphasic wall structure, with a fibrous inner layer and a semipermeable outer layer. The swelling properties and the mechanical strength before and after in vitro degradation were investigated. The biocompatibility of the scaffolds was also investigated by co-culturing neuroblastoma cells (N2A, mouse) with the scaffolds. The results suggest that these chitosan tubular scaffolds are useful for the regeneration of tissues requiring a tubular scaffold.",
keywords = "Chitosan, Knitting, Nerve conduit, Tissue engineering",
author = "Aijun Wang and Qiang Ao and Wenling Cao and Chang Zhao and Yandao Gong and Nanming Zhao and Xiufang Zhang",
year = "2005",
month = "8",
doi = "10.1016/S1007-0214(05)70099-7",
language = "English (US)",
volume = "10",
pages = "449--453",
journal = "Tsinghua Science and Technology",
issn = "1007-0214",
publisher = "Tsing Hua University",
number = "4",

}

TY - JOUR

T1 - Fiber-based chitosan tubular scaffolds for soft tissue engineering

T2 - Fabrication and in vitro evaluation

AU - Wang, Aijun

AU - Ao, Qiang

AU - Cao, Wenling

AU - Zhao, Chang

AU - Gong, Yandao

AU - Zhao, Nanming

AU - Zhang, Xiufang

PY - 2005/8

Y1 - 2005/8

N2 - Porous, two-ply tubular chitosan conduits for guided tissue regeneration were fabricated by combining the textile technique (inner layer) with the thermally induced phase separation process (outer layer). A hollow chitosan tube was prepared using an industrial warp knitting process with chitosan yarns. Then, an appropriate diameter mandrel was inserted into the pre-fabricated tube. The tube and the mandrel were dipped into the chitosan solution together, taken out, and freeze-dried. After being neutralized in alkaline solution and dried at room temperature, the mandrel was removed to create the chitosan tubular scaffold. Scanning electron micrographs show that the resulting tubes have a biphasic wall structure, with a fibrous inner layer and a semipermeable outer layer. The swelling properties and the mechanical strength before and after in vitro degradation were investigated. The biocompatibility of the scaffolds was also investigated by co-culturing neuroblastoma cells (N2A, mouse) with the scaffolds. The results suggest that these chitosan tubular scaffolds are useful for the regeneration of tissues requiring a tubular scaffold.

AB - Porous, two-ply tubular chitosan conduits for guided tissue regeneration were fabricated by combining the textile technique (inner layer) with the thermally induced phase separation process (outer layer). A hollow chitosan tube was prepared using an industrial warp knitting process with chitosan yarns. Then, an appropriate diameter mandrel was inserted into the pre-fabricated tube. The tube and the mandrel were dipped into the chitosan solution together, taken out, and freeze-dried. After being neutralized in alkaline solution and dried at room temperature, the mandrel was removed to create the chitosan tubular scaffold. Scanning electron micrographs show that the resulting tubes have a biphasic wall structure, with a fibrous inner layer and a semipermeable outer layer. The swelling properties and the mechanical strength before and after in vitro degradation were investigated. The biocompatibility of the scaffolds was also investigated by co-culturing neuroblastoma cells (N2A, mouse) with the scaffolds. The results suggest that these chitosan tubular scaffolds are useful for the regeneration of tissues requiring a tubular scaffold.

KW - Chitosan

KW - Knitting

KW - Nerve conduit

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=24044487175&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24044487175&partnerID=8YFLogxK

U2 - 10.1016/S1007-0214(05)70099-7

DO - 10.1016/S1007-0214(05)70099-7

M3 - Article

AN - SCOPUS:24044487175

VL - 10

SP - 449

EP - 453

JO - Tsinghua Science and Technology

JF - Tsinghua Science and Technology

SN - 1007-0214

IS - 4

ER -