Fetal surgery for repair of myelomeningocele allows normal development of the rectum in sheep

Jyoji Yoshizawa, Lourenco Sbragia, Bettina W. Paek, Roman M. Sydorak, Yoji Yamazaki, Michael R. Harrison, Diana L Farmer

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To determine whether fetal surgery in a fetus with myelomeningocele (MMC) allows normal development of rectal muscles and nerves, we analyzed the rectum after fetal surgery in a sheep model. An MMC lesion was surgically created in 13 fetal sheep at 75 days of gestation. One fetal sheep died after the lesion was created. Eight fetal sheep were repaired at 100 days of gestation; the others were not repaired, as a control (n=4). Three methods were used for fetal surgery of MMC: standard neurosurgical repair (4 fetal sheep), covering of the MMC lesion by Alloderm (2 fetal sheep), and covering of the MMC lesion by Gore-Tex (2 fetal sheep). At 140 days of gestation, fetal sheep were harvested and histo-pathological analysis was performed on the rectum using hematoxylin and eosin staining for muscles and S-100 protein staining for nerves. One fetal sheep treated by standard neurosurgical repair died before harvesting. The four untreated fetal sheep had hypoplastic longitudinal muscles of the rectum but well developed-circular muscles. In addition, the untreated fetuses had a hypoplastic submucosal nerve plexus but a well-developed intermuscular nerve plexus. In contrast, treated fetal sheep had well-developed longitudinal and circular muscles except for one sheep treated with standard neurosurgical repair. In addition, except for the same fetal sheep, treated fetal sheep had well-developed nerve plexuses. There was no difference in muscle and nerve development of the rectum among the three repair methods. Fetal surgery for repair of MMC allows normal development of rectal muscles and nerves.

Original languageEnglish (US)
Pages (from-to)162-166
Number of pages5
JournalPediatric Surgery International
Volume19
Issue number3
StatePublished - May 2003

Fingerprint

Meningomyelocele
Rectum
Sheep
Muscle Development
Muscles
Pregnancy
Fetus
Submucous Plexus
Staining and Labeling
S100 Proteins
Hematoxylin
Eosine Yellowish-(YS)

Keywords

  • Fetal surgey
  • Incontinence
  • Myelomeningocele
  • Rectum

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Yoshizawa, J., Sbragia, L., Paek, B. W., Sydorak, R. M., Yamazaki, Y., Harrison, M. R., & Farmer, D. L. (2003). Fetal surgery for repair of myelomeningocele allows normal development of the rectum in sheep. Pediatric Surgery International, 19(3), 162-166.

Fetal surgery for repair of myelomeningocele allows normal development of the rectum in sheep. / Yoshizawa, Jyoji; Sbragia, Lourenco; Paek, Bettina W.; Sydorak, Roman M.; Yamazaki, Yoji; Harrison, Michael R.; Farmer, Diana L.

In: Pediatric Surgery International, Vol. 19, No. 3, 05.2003, p. 162-166.

Research output: Contribution to journalArticle

Yoshizawa, J, Sbragia, L, Paek, BW, Sydorak, RM, Yamazaki, Y, Harrison, MR & Farmer, DL 2003, 'Fetal surgery for repair of myelomeningocele allows normal development of the rectum in sheep', Pediatric Surgery International, vol. 19, no. 3, pp. 162-166.
Yoshizawa J, Sbragia L, Paek BW, Sydorak RM, Yamazaki Y, Harrison MR et al. Fetal surgery for repair of myelomeningocele allows normal development of the rectum in sheep. Pediatric Surgery International. 2003 May;19(3):162-166.
Yoshizawa, Jyoji ; Sbragia, Lourenco ; Paek, Bettina W. ; Sydorak, Roman M. ; Yamazaki, Yoji ; Harrison, Michael R. ; Farmer, Diana L. / Fetal surgery for repair of myelomeningocele allows normal development of the rectum in sheep. In: Pediatric Surgery International. 2003 ; Vol. 19, No. 3. pp. 162-166.
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