Fetal surgery for repair of myelomeningocele allows normal development of anal sphincter muscles in sheep

Jyoji Yoshizawa, Lourenco Sbragia, Bettina W. Paek, Roman M. Sydorak, Yoji Yamazaki, Michael R. Harrison, Diana L Farmer

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

One major problem for patients with myelomeningocele (MMC) is fecal incontinence. To prevent this problem, fetal surgery for repair of MMC has been recently undertaken. The strategy behind this surgery is to allow normal development of anal sphincter muscles. The purpose of this study was to determine whether fetal surgery for repair of MMC allows normal development of anal sphincter muscles. Myelomeningocele was surgically created in fetal sheep at 75 days of gestation. At 100 days of gestation, fetal surgery for repair of the MMC lesion was performed. Three repair methods were used: standard neurosurgical repair (4 fetal sheep), covering the MMC lesion with Alloderm (2 fetal sheep), and covering the MMC lesion with Gore-Tex (2 fetal sheep). After the sheep were delivered (140 days of gestation), external and internal anal sphincter muscles were analyzed histopathologically. In control fetal sheep (not repaired) anal sphincter muscles did not develop normally. In contrast, in fetal sheep that underwent repair of the MMC, the external and internal anal sphincter muscles developed normally. Histopathologically, in the external sphincter muscles, muscle fibers were dense. In the internal sphincter muscles, endomysial spaces were small, myofibrils were numerous, and fascicular units were larger than those in unrepaired fetal sheep. There was no difference in muscle development for the repair methods. Fetal surgery for repair of MMC allows normal development of anal sphincter muscles.

Original languageEnglish (US)
Pages (from-to)14-18
Number of pages5
JournalPediatric Surgery International
Volume20
Issue number1
DOIs
StatePublished - Jan 2004

Fingerprint

Meningomyelocele
Anal Canal
Sheep
Muscles
Pregnancy
Fecal Incontinence
Muscle Development
Myofibrils

Keywords

  • Anal sphincter muscle
  • Fetal surgery
  • Myelomeningocele

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Fetal surgery for repair of myelomeningocele allows normal development of anal sphincter muscles in sheep. / Yoshizawa, Jyoji; Sbragia, Lourenco; Paek, Bettina W.; Sydorak, Roman M.; Yamazaki, Yoji; Harrison, Michael R.; Farmer, Diana L.

In: Pediatric Surgery International, Vol. 20, No. 1, 01.2004, p. 14-18.

Research output: Contribution to journalArticle

Yoshizawa, Jyoji ; Sbragia, Lourenco ; Paek, Bettina W. ; Sydorak, Roman M. ; Yamazaki, Yoji ; Harrison, Michael R. ; Farmer, Diana L. / Fetal surgery for repair of myelomeningocele allows normal development of anal sphincter muscles in sheep. In: Pediatric Surgery International. 2004 ; Vol. 20, No. 1. pp. 14-18.
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