Fetal monkey surfactants after intra-amniotic or maternal administration of betamethasone and thyroid hormone

William M. Gilbert, Elaine Eby-Wilkens, Charles Plopper, Jeffery A. Whitsett, Alice F Tarantal

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

OBJECTIVE: To compare direct intra-amniotic injection of betamethasone and thyroxine (T4) with maternal treatment and controls for accelerating pulmonary surfactant production. METHODS: Twelve pregnant monkeys (Macaca mulatta) on gestational day 125 (term 165 ± 10 days) had surfactant protein A and B concentrations measured in amniotic fluid. In four controls, normal saline was injected into the amniotic fluid; four others (intra-amniotic) received intra-amniotic betamethasone (1 mg) and T4 (60 μg); and in four others (maternal), the dam was given betamethasone (12 mg) intramuscularly, repeated in 24 hours, plus TRH (400 μg) intravenously, repeated every 6 hours for 24 hours. Seventy-two hours after the initial amniocentesis, a hysterotomy was performed and fetal tissue and amniotic fluid harvested for determination of surfactant protein A and B concentrations and immunohistochemical staining for surfactant protein A. RESULTS: Amniotic fluid surfactant protein A was higher with intra-amniotic injection than with maternal treatment (P < .04) or controls (P = .07). Amniotic fluid surfactant protein B was higher in the intra-amniotic group than in controls (P = .06). Immunohistochemical staining for surfactant protein A in the lung tissue was increased in the intra-amniotic group compared with controls (0.145 ± 0.01 versus 0.097 ± 0.001, percent positive staining for surfactant protein A cells per lung tissue cells; P < .03). Birth weight was greater in the intra-amniotic group compared with the maternal group (P < .03) although not different from the controls. Finally, gut motility and the presence of formed meconium were increased in the intra-amniotic group compared with the other groups (P < .05). CONCLUSION: Intra-amniotic injection of betamethasone and T4 enhanced lung (and possibly intestinal) maturation of the preterm rhesus fetal monkey compared with maternal injections.

Original languageEnglish (US)
Pages (from-to)466-470
Number of pages5
JournalObstetrics and Gynecology
Volume98
Issue number3
DOIs
StatePublished - 2001

Fingerprint

Pulmonary Surfactant-Associated Protein A
Betamethasone
Thyroid Hormones
Surface-Active Agents
Haplorhini
Amniotic Fluid
Mothers
Injections
Staining and Labeling
Macaca mulatta
Lung
Hysterotomy
Pulmonary Surfactants
Meconium
Amniocentesis
Thyroxine
Birth Weight
Fetus
Control Groups
Therapeutics

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Fetal monkey surfactants after intra-amniotic or maternal administration of betamethasone and thyroid hormone. / Gilbert, William M.; Eby-Wilkens, Elaine; Plopper, Charles; Whitsett, Jeffery A.; Tarantal, Alice F.

In: Obstetrics and Gynecology, Vol. 98, No. 3, 2001, p. 466-470.

Research output: Contribution to journalArticle

@article{2b1b07353abb4dbf97bde24bcbd524cd,
title = "Fetal monkey surfactants after intra-amniotic or maternal administration of betamethasone and thyroid hormone",
abstract = "OBJECTIVE: To compare direct intra-amniotic injection of betamethasone and thyroxine (T4) with maternal treatment and controls for accelerating pulmonary surfactant production. METHODS: Twelve pregnant monkeys (Macaca mulatta) on gestational day 125 (term 165 ± 10 days) had surfactant protein A and B concentrations measured in amniotic fluid. In four controls, normal saline was injected into the amniotic fluid; four others (intra-amniotic) received intra-amniotic betamethasone (1 mg) and T4 (60 μg); and in four others (maternal), the dam was given betamethasone (12 mg) intramuscularly, repeated in 24 hours, plus TRH (400 μg) intravenously, repeated every 6 hours for 24 hours. Seventy-two hours after the initial amniocentesis, a hysterotomy was performed and fetal tissue and amniotic fluid harvested for determination of surfactant protein A and B concentrations and immunohistochemical staining for surfactant protein A. RESULTS: Amniotic fluid surfactant protein A was higher with intra-amniotic injection than with maternal treatment (P < .04) or controls (P = .07). Amniotic fluid surfactant protein B was higher in the intra-amniotic group than in controls (P = .06). Immunohistochemical staining for surfactant protein A in the lung tissue was increased in the intra-amniotic group compared with controls (0.145 ± 0.01 versus 0.097 ± 0.001, percent positive staining for surfactant protein A cells per lung tissue cells; P < .03). Birth weight was greater in the intra-amniotic group compared with the maternal group (P < .03) although not different from the controls. Finally, gut motility and the presence of formed meconium were increased in the intra-amniotic group compared with the other groups (P < .05). CONCLUSION: Intra-amniotic injection of betamethasone and T4 enhanced lung (and possibly intestinal) maturation of the preterm rhesus fetal monkey compared with maternal injections.",
author = "Gilbert, {William M.} and Elaine Eby-Wilkens and Charles Plopper and Whitsett, {Jeffery A.} and Tarantal, {Alice F}",
year = "2001",
doi = "10.1016/S0029-7844(01)01417-X",
language = "English (US)",
volume = "98",
pages = "466--470",
journal = "Obstetrics and Gynecology",
issn = "0029-7844",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Fetal monkey surfactants after intra-amniotic or maternal administration of betamethasone and thyroid hormone

AU - Gilbert, William M.

AU - Eby-Wilkens, Elaine

AU - Plopper, Charles

AU - Whitsett, Jeffery A.

AU - Tarantal, Alice F

PY - 2001

Y1 - 2001

N2 - OBJECTIVE: To compare direct intra-amniotic injection of betamethasone and thyroxine (T4) with maternal treatment and controls for accelerating pulmonary surfactant production. METHODS: Twelve pregnant monkeys (Macaca mulatta) on gestational day 125 (term 165 ± 10 days) had surfactant protein A and B concentrations measured in amniotic fluid. In four controls, normal saline was injected into the amniotic fluid; four others (intra-amniotic) received intra-amniotic betamethasone (1 mg) and T4 (60 μg); and in four others (maternal), the dam was given betamethasone (12 mg) intramuscularly, repeated in 24 hours, plus TRH (400 μg) intravenously, repeated every 6 hours for 24 hours. Seventy-two hours after the initial amniocentesis, a hysterotomy was performed and fetal tissue and amniotic fluid harvested for determination of surfactant protein A and B concentrations and immunohistochemical staining for surfactant protein A. RESULTS: Amniotic fluid surfactant protein A was higher with intra-amniotic injection than with maternal treatment (P < .04) or controls (P = .07). Amniotic fluid surfactant protein B was higher in the intra-amniotic group than in controls (P = .06). Immunohistochemical staining for surfactant protein A in the lung tissue was increased in the intra-amniotic group compared with controls (0.145 ± 0.01 versus 0.097 ± 0.001, percent positive staining for surfactant protein A cells per lung tissue cells; P < .03). Birth weight was greater in the intra-amniotic group compared with the maternal group (P < .03) although not different from the controls. Finally, gut motility and the presence of formed meconium were increased in the intra-amniotic group compared with the other groups (P < .05). CONCLUSION: Intra-amniotic injection of betamethasone and T4 enhanced lung (and possibly intestinal) maturation of the preterm rhesus fetal monkey compared with maternal injections.

AB - OBJECTIVE: To compare direct intra-amniotic injection of betamethasone and thyroxine (T4) with maternal treatment and controls for accelerating pulmonary surfactant production. METHODS: Twelve pregnant monkeys (Macaca mulatta) on gestational day 125 (term 165 ± 10 days) had surfactant protein A and B concentrations measured in amniotic fluid. In four controls, normal saline was injected into the amniotic fluid; four others (intra-amniotic) received intra-amniotic betamethasone (1 mg) and T4 (60 μg); and in four others (maternal), the dam was given betamethasone (12 mg) intramuscularly, repeated in 24 hours, plus TRH (400 μg) intravenously, repeated every 6 hours for 24 hours. Seventy-two hours after the initial amniocentesis, a hysterotomy was performed and fetal tissue and amniotic fluid harvested for determination of surfactant protein A and B concentrations and immunohistochemical staining for surfactant protein A. RESULTS: Amniotic fluid surfactant protein A was higher with intra-amniotic injection than with maternal treatment (P < .04) or controls (P = .07). Amniotic fluid surfactant protein B was higher in the intra-amniotic group than in controls (P = .06). Immunohistochemical staining for surfactant protein A in the lung tissue was increased in the intra-amniotic group compared with controls (0.145 ± 0.01 versus 0.097 ± 0.001, percent positive staining for surfactant protein A cells per lung tissue cells; P < .03). Birth weight was greater in the intra-amniotic group compared with the maternal group (P < .03) although not different from the controls. Finally, gut motility and the presence of formed meconium were increased in the intra-amniotic group compared with the other groups (P < .05). CONCLUSION: Intra-amniotic injection of betamethasone and T4 enhanced lung (and possibly intestinal) maturation of the preterm rhesus fetal monkey compared with maternal injections.

UR - http://www.scopus.com/inward/record.url?scp=0034836246&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034836246&partnerID=8YFLogxK

U2 - 10.1016/S0029-7844(01)01417-X

DO - 10.1016/S0029-7844(01)01417-X

M3 - Article

C2 - 11530131

AN - SCOPUS:0034836246

VL - 98

SP - 466

EP - 470

JO - Obstetrics and Gynecology

JF - Obstetrics and Gynecology

SN - 0029-7844

IS - 3

ER -