Fetal gender determination in early first trimester pregnancies of rhesus monkeys (Macaca mulatta) by flourescent PCR analysis of maternal serum

Daniel F. Jimenez, Alice F Tarantal

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Non-human primate fetal gender determination can be a powerful tool for research study design and colony management purposes. The recent discovery of the presence of fetal DNA in maternal serum has offered a new non-invasive approach for identification of fetal gender. We present a rapid and simple method for the sexing of developing rhesus monkeys in the first trimester by polymerase chain reaction (PCR) analysis of maternal serum. Serum samples were obtained from 72 gravid rhesus monkeys during 20-32 days of gestation (term 165 ± 10 days). Fetal gender and the quantity of circulating fetal DNA were determined by real-time PCR analysis of the rhesus Y-chromosomal DNA sequences. The sensitivity for identifying a male fetus was 100% by 30 days gestation, and no false-positive results were observed. This study demonstrates that fetal gender can be reliably determined in the early first trimester from maternal serum samples, a non-invasive method for routine gender screening.

Original languageEnglish (US)
Pages (from-to)315-319
Number of pages5
JournalJournal of Medical Primatology
Volume32
Issue number6
DOIs
StatePublished - Dec 2003

Fingerprint

First Pregnancy Trimester
Macaca mulatta
polymerase chain reaction
Mothers
pregnancy
Polymerase Chain Reaction
gender
Serum
Pregnancy
DNA
Primates
sex determination analysis
Real-Time Polymerase Chain Reaction
Fetus
Research Design
fetus
quantitative polymerase chain reaction
experimental design
screening
nucleotide sequences

Keywords

  • Cell-free fetal DNA
  • Maternal blood
  • Tarnsplancental transfer
  • Y chromosome

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

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