Fetal exposure of rhesus macaques to bisphenol a alters cellular development of the conducting airway by changing epithelial secretory product expression

Laura S. Van Winkle, Shannon R. Murphy, Miriam V. Boetticher, Catherine A. VandeVoort

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background: Bisphenol A (BPA) exposure early in life results in organizational changes in reproductive organs, but the effect of BPA on conducting airway cellular maturation has not been studied. Late gestation is characterized by active differentiation of secretory cells in the lung epithelium. Objective: We evaluated the hypothesis that BPA exposure disrupts epithelial secretory cell development in the fetal conducting airway of the rhesus macaque. Methods: We exposed animals to BPA during either the second (early term) or the third (late term) trimester. There were four treatment groups: a) sham control early term, b) sham control late term, c) BPA early term (BPA-early), and d) BPA late term (BPA-late). Because cellular maturation occurs nonuniformly in the lung, we defined mRNA and protein expression by airway level using microdissection. Results: BPA exposure of the dam during late term significantly accelerated secretory cell maturation in the proximal airways of the fetus; both Clara cell secretory protein (CCSP) and MUC5AC/5B mRNA and protein expression increased. Conclusions: BPA exposure during late gestation accelerates secretory cell maturation in the proximal conducting airways. We identified a critical window of fetal susceptibility for BPA effects on lung epithelial cell maturation in the third trimester. This is of environmental health importance because increases in airway mucins are hallmarks of a number of childhood lung diseases that may be affected by BPA exposure.

Original languageEnglish (US)
Pages (from-to)912-918
Number of pages7
JournalEnvironmental Health Perspectives
Volume121
Issue number8
DOIs
StatePublished - Aug 2013

Fingerprint

Macaca mulatta
Lung
Epithelial Cells
Uteroglobin
bisphenol A
Organizational Innovation
Pregnancy
Microdissection
Messenger RNA
Environmental Health
Third Pregnancy Trimester
Mucins
Fetal Development
Lung Diseases
Cell Differentiation
Proteins
Fetus
Epithelium

Keywords

  • CC10
  • CC16
  • Lung development
  • Macaca mulatta
  • Mucin
  • Prenatal
  • SCGB1A1

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health

Cite this

Fetal exposure of rhesus macaques to bisphenol a alters cellular development of the conducting airway by changing epithelial secretory product expression. / Van Winkle, Laura S.; Murphy, Shannon R.; Boetticher, Miriam V.; VandeVoort, Catherine A.

In: Environmental Health Perspectives, Vol. 121, No. 8, 08.2013, p. 912-918.

Research output: Contribution to journalArticle

Van Winkle, Laura S. ; Murphy, Shannon R. ; Boetticher, Miriam V. ; VandeVoort, Catherine A. / Fetal exposure of rhesus macaques to bisphenol a alters cellular development of the conducting airway by changing epithelial secretory product expression. In: Environmental Health Perspectives. 2013 ; Vol. 121, No. 8. pp. 912-918.
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