Fetal and postnatal ovine mesenteric vascular reactivity

Jayasree Nair, Sylvia F. Gugino, Lori C. Nielsen, Michael G. Caty, Satyanarayana Lakshminrusimha

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background:Intestinal circulation and mesenteric arterial (MA) reactivity may play a role in preparing the fetus for enteral nutrition. We hypothesized that MA vasoreactivity changes with gestation and vasodilator pathways predominate in the postnatal period.Methods:Small distal MA rings (0.5-mm diameter) were isolated from fetal (116-d, 128-d, 134-d, and 141-d gestation, term ∼ 147 d) and postnatal lambs. Vasoreactivity was evaluated using vasoconstrictors (norepinephrine (NE) after pretreatment with propranolol and endothelin-1(ET-1)) and vasodilators (NO donors A23187 and s-nitrosopenicillamine (SNAP)). Protein and mRNA assays for receptors and enzymes (endothelin receptor A, alpha-adrenergic receptor 1A (ADRA1A), endothelial NO synthase (eNOS), soluble guanylyl cyclase (sGC), and phosphodiesterase5 (PDE5)) were performed in mesenteric arteries.Results:MA constriction to NE and ET-1 peaked at 134 d. Relaxation to A23187 and SNAP was maximal after birth. Basal eNOS activity was low at 134 d. ADRA1A mRNA and protein increased significantly at 134 d and decreased postnatally. sGC and PDE5 protein increased from 134 to 141 d.Conclusion:Mesenteric vasoconstriction predominates in late-preterm gestation (134 d; the postconceptional age with the highest incidence of necrotizing enterocolitis (NEC)) followed by a conversion to vasodilatory influences near the time of full-term birth. Perturbations in this ontogenic mechanism, including preterm birth, may be a risk factor for NEC.

Original languageEnglish (US)
Pages (from-to)575-582
Number of pages8
JournalPediatric Research
Volume79
Issue number4
DOIs
StatePublished - Apr 1 2016
Externally publishedYes

Fingerprint

Blood Vessels
Sheep
Necrotizing Enterocolitis
Calcimycin
Endothelin-1
Vasodilator Agents
Nitric Oxide Synthase
Pregnancy
Norepinephrine
Adrenergic alpha-1 Receptors
Splanchnic Circulation
Term Birth
Endothelin A Receptors
Receptors, Adrenergic, alpha
Messenger RNA
Proteins
Mesenteric Arteries
Premature Birth
Enzyme Assays
Enteral Nutrition

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Fetal and postnatal ovine mesenteric vascular reactivity. / Nair, Jayasree; Gugino, Sylvia F.; Nielsen, Lori C.; Caty, Michael G.; Lakshminrusimha, Satyanarayana.

In: Pediatric Research, Vol. 79, No. 4, 01.04.2016, p. 575-582.

Research output: Contribution to journalArticle

Nair, Jayasree ; Gugino, Sylvia F. ; Nielsen, Lori C. ; Caty, Michael G. ; Lakshminrusimha, Satyanarayana. / Fetal and postnatal ovine mesenteric vascular reactivity. In: Pediatric Research. 2016 ; Vol. 79, No. 4. pp. 575-582.
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AU - Lakshminrusimha, Satyanarayana

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AB - Background:Intestinal circulation and mesenteric arterial (MA) reactivity may play a role in preparing the fetus for enteral nutrition. We hypothesized that MA vasoreactivity changes with gestation and vasodilator pathways predominate in the postnatal period.Methods:Small distal MA rings (0.5-mm diameter) were isolated from fetal (116-d, 128-d, 134-d, and 141-d gestation, term ∼ 147 d) and postnatal lambs. Vasoreactivity was evaluated using vasoconstrictors (norepinephrine (NE) after pretreatment with propranolol and endothelin-1(ET-1)) and vasodilators (NO donors A23187 and s-nitrosopenicillamine (SNAP)). Protein and mRNA assays for receptors and enzymes (endothelin receptor A, alpha-adrenergic receptor 1A (ADRA1A), endothelial NO synthase (eNOS), soluble guanylyl cyclase (sGC), and phosphodiesterase5 (PDE5)) were performed in mesenteric arteries.Results:MA constriction to NE and ET-1 peaked at 134 d. Relaxation to A23187 and SNAP was maximal after birth. Basal eNOS activity was low at 134 d. ADRA1A mRNA and protein increased significantly at 134 d and decreased postnatally. sGC and PDE5 protein increased from 134 to 141 d.Conclusion:Mesenteric vasoconstriction predominates in late-preterm gestation (134 d; the postconceptional age with the highest incidence of necrotizing enterocolitis (NEC)) followed by a conversion to vasodilatory influences near the time of full-term birth. Perturbations in this ontogenic mechanism, including preterm birth, may be a risk factor for NEC.

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