Fertilizing capacity of equine spermatozoa stored for 24 hours at 5 or 20°C

D. D. Varner, T. L. Blanchard, P. J. Meyers, Stuart A Meyers

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

A breeding trial was conducted to evaluate the effect of in vitro storage time and temperature on fertilizing capacity of equine spermatozoa. Semen obtained from one stallion and diluted with skim milk-glucose extender was used to artificially inseminate 45 estrussynchronized mares. The mares were assigned to one of three treatment groups (15 mares per group): 1) insemination with fresh semen (collected within 0.5 h of use), 2) insemination with semen stored for 24 h at 20°C or 3) insemination with semen stored for 24 h at 5°C. The mares were inseminated daily during estrus, from the detection of a 35-mm follicle until ovulation, with 250 × 106 progressively motile spermatozoa (based on initial sperm motility of fresh semen). Semen samples (n = 35) were evaluated prior to insemination for percentages of total sperm motility (TSM), progressive sperm motility (PSM) and sperm velocity (SV). Single-cycle 15-d pregnancy rates. resulting from insemination with fresh semen, from fresh semen stored for 24 h at 20°C or from semen stored for 24 h at 5°C were the same ( 11 15; 73%). Mean diameters (mm) of 15-d embryonic vesicles were not different (P>0.05) among these three treatment groups (21.5 ± 2.9, 19.6 ± 2.6 and 20.5 ± 3.6, respectively). Ten pregnant mares were aborted on Day 15 of gestation for use in another project. The pregnancy status of the 23 remaining pregnant mares was again determined at 35 to 40 d and 55 to 60 d of gestation. No pregnancy losses occurred during this time period. Mean TSM percentages were different (P<0.05) among the three groups: the fresh semen percentage was 89 ± 2, semen stored for 24 h at 20°C was 57 ± 11 and semen stored for 24 h at 5°C was 80 ± 6. Similar differences were found for mean PSM and SV. Semen storage at either 20 or 5°C for 24 h had no apparent effect on the fertilizing capacity of the extended semen samples; however, the reduction in all motility parameters tested was more dramatic in semen stored at 20°C than that stored at 5°C.

Original languageEnglish (US)
Pages (from-to)515-525
Number of pages11
JournalTheriogenology
Volume32
Issue number4
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Semen
Horses
Spermatozoa
semen
spermatozoa
horses
Sperm Motility
Insemination
mares
sperm motility
insemination
pregnancy
Pregnancy
Estrus Detection
Pregnancy Rate
skim milk
stallions
Ovulation
pregnancy rate
storage temperature

Keywords

  • fertility
  • motility
  • semen storage
  • spermatozoa
  • stallion

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Fertilizing capacity of equine spermatozoa stored for 24 hours at 5 or 20°C. / Varner, D. D.; Blanchard, T. L.; Meyers, P. J.; Meyers, Stuart A.

In: Theriogenology, Vol. 32, No. 4, 1989, p. 515-525.

Research output: Contribution to journalArticle

Varner, D. D. ; Blanchard, T. L. ; Meyers, P. J. ; Meyers, Stuart A. / Fertilizing capacity of equine spermatozoa stored for 24 hours at 5 or 20°C. In: Theriogenology. 1989 ; Vol. 32, No. 4. pp. 515-525.
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