Fertilization initiates the transition from anaphase I to metaphase II during female meiosis in C. elegans

Karen L. McNally, Francis J. McNally

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Oocytes from most animals arrest twice during the meiotic cell cycle. The universally conserved prophase I arrest is released by a maturation hormone that allows progression to a second arrest point, typically metaphase I or II. This second arrest allows for short-term storage of fertilization-competent eggs and is released by signaling that occurs during fertilization. Nematodes are unique in that the maturation hormone is secreted by sperm rather than by the mother's somatic tissues. We have investigated the nature of the second arrest in matured but unfertilized Caenorhabditis elegans embryos using time-lapse imaging of GFP-tubulin or GFP-histone. Unfertilized embryos completed anaphase I but did not form polar bodies or assemble meiosis II spindles. Nevertheless, unfertilized embryos assembled female pronuclei at the same time as fertilized embryos. Analysis of embryos fertilized by sperm lacking the SPE-11 protein indicated that fertilization promotes meiotic cytokinesis through the SPE-11 protein but assembly of the meiosis II spindle is initiated through an SPE-11-independent pathway.

Original languageEnglish (US)
Pages (from-to)218-230
Number of pages13
JournalDevelopmental Biology
Volume282
Issue number1
DOIs
StatePublished - Jun 1 2005

Fingerprint

Anaphase
Meiosis
Metaphase
Fertilization
Embryonic Structures
Spermatozoa
Time-Lapse Imaging
Hormones
Meiotic Prophase I
Polar Bodies
Cytokinesis
Caenorhabditis elegans
Tubulin
Histones
Eggs
Oocytes
Cell Cycle
Proteins

Keywords

  • Cyclin B
  • Fertilization
  • Meiosis
  • Meiotic exit

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Fertilization initiates the transition from anaphase I to metaphase II during female meiosis in C. elegans. / McNally, Karen L.; McNally, Francis J.

In: Developmental Biology, Vol. 282, No. 1, 01.06.2005, p. 218-230.

Research output: Contribution to journalArticle

McNally, Karen L. ; McNally, Francis J. / Fertilization initiates the transition from anaphase I to metaphase II during female meiosis in C. elegans. In: Developmental Biology. 2005 ; Vol. 282, No. 1. pp. 218-230.
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