Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background grapheme

Carolin Seuring; Kartik Ayyer; Eleftheria Filippaki; Miriam Barthelmess; Jean Nicolas Longchamp; Philippe Ringler; Tommaso Pardini; David H. Wojtas; Matthew A Coleman; Katerina Dörner; Silje Fuglerud; Greger Hammarin; Birgit Habenstein; Annette E. Langkilde; Antoine Loquet; Alke Meents; Roland Riek; Henning Stahlberg; Sébastien Boutet; Mark S. Hunter; Jason Koglin; Mengning Liang; Helen M. Ginn; Rick P. Millane; Matthias Frank; Anton Barty; Henry N. Chapman

doi:10.1038/s41467-018-04116-9

Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background grapheme

Carolin Seuring, Kartik Ayyer, Eleftheria Filippaki, Miriam Barthelmess, Jean Nicolas Longchamp, Philippe Ringler, Tommaso Pardini, David H. Wojtas, Matthew A Coleman, Katerina Dörner, Silje Fuglerud, Greger Hammarin, Birgit Habenstein, Annette E. Langkilde, Antoine Loquet, Alke Meents, Roland Riek, Henning Stahlberg, Sébastien Boutet, Mark S. HunterJason Koglin, Mengning Liang, Helen M. Ginn, Rick P. Millane, Matthias Frank, Anton Barty, Henry N. Chapman

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.

Original language	English (US)
Article number	1836
Journal	Nature Communications
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41467-018-04116-9
State	Published - Dec 1 2018
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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10.1038/s41467-018-04116-9

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Seuring, C., Ayyer, K., Filippaki, E., Barthelmess, M., Longchamp, J. N., Ringler, P., Pardini, T., Wojtas, D. H., Coleman, M. A., Dörner, K., Fuglerud, S., Hammarin, G., Habenstein, B., Langkilde, A. E., Loquet, A., Meents, A., Riek, R., Stahlberg, H., Boutet, S., ... Chapman, H. N. (2018). Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background grapheme. Nature Communications, 9(1), [1836]. https://doi.org/10.1038/s41467-018-04116-9

Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background grapheme. / Seuring, Carolin; Ayyer, Kartik; Filippaki, Eleftheria; Barthelmess, Miriam; Longchamp, Jean Nicolas; Ringler, Philippe; Pardini, Tommaso; Wojtas, David H.; Coleman, Matthew A; Dörner, Katerina; Fuglerud, Silje; Hammarin, Greger; Habenstein, Birgit; Langkilde, Annette E.; Loquet, Antoine; Meents, Alke; Riek, Roland; Stahlberg, Henning; Boutet, Sébastien; Hunter, Mark S.; Koglin, Jason; Liang, Mengning; Ginn, Helen M.; Millane, Rick P.; Frank, Matthias; Barty, Anton; Chapman, Henry N.

In: Nature Communications, Vol. 9, No. 1, 1836, 01.12.2018.

Research output: Contribution to journal › Article › peer-review

Seuring, C, Ayyer, K, Filippaki, E, Barthelmess, M, Longchamp, JN, Ringler, P, Pardini, T, Wojtas, DH, Coleman, MA, Dörner, K, Fuglerud, S, Hammarin, G, Habenstein, B, Langkilde, AE, Loquet, A, Meents, A, Riek, R, Stahlberg, H, Boutet, S, Hunter, MS, Koglin, J, Liang, M, Ginn, HM, Millane, RP, Frank, M, Barty, A & Chapman, HN 2018, 'Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background grapheme', Nature Communications, vol. 9, no. 1, 1836. https://doi.org/10.1038/s41467-018-04116-9

Seuring, Carolin ; Ayyer, Kartik ; Filippaki, Eleftheria ; Barthelmess, Miriam ; Longchamp, Jean Nicolas ; Ringler, Philippe ; Pardini, Tommaso ; Wojtas, David H. ; Coleman, Matthew A ; Dörner, Katerina ; Fuglerud, Silje ; Hammarin, Greger ; Habenstein, Birgit ; Langkilde, Annette E. ; Loquet, Antoine ; Meents, Alke ; Riek, Roland ; Stahlberg, Henning ; Boutet, Sébastien ; Hunter, Mark S. ; Koglin, Jason ; Liang, Mengning ; Ginn, Helen M. ; Millane, Rick P. ; Frank, Matthias ; Barty, Anton ; Chapman, Henry N. / Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background grapheme. In: Nature Communications. 2018 ; Vol. 9, No. 1.

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abstract = "Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 {\AA} and 2.4 {\AA} resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.",

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AU - Ringler, Philippe

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AU - Hunter, Mark S.

AU - Koglin, Jason

AU - Liang, Mengning

AU - Ginn, Helen M.

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AU - Frank, Matthias

AU - Barty, Anton

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Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background grapheme

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