Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background grapheme

Carolin Seuring, Kartik Ayyer, Eleftheria Filippaki, Miriam Barthelmess, Jean Nicolas Longchamp, Philippe Ringler, Tommaso Pardini, David H. Wojtas, Matthew A Coleman, Katerina Dörner, Silje Fuglerud, Greger Hammarin, Birgit Habenstein, Annette E. Langkilde, Antoine Loquet, Alke Meents, Roland Riek, Henning Stahlberg, Sébastien Boutet, Mark S. Hunter & 7 others Jason Koglin, Mengning Liang, Helen M. Ginn, Rick P. Millane, Matthias Frank, Anton Barty, Henry N. Chapman

Research output: Contribution to journal › Article

9 Citations (Scopus)

Abstract

Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.

Original languageEnglish (US)
Article number1836
JournalNature Communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018
Externally publishedYes

Fingerprint

Tobacco Mosaic Virus
Graphite
Amyloid
X-Ray Diffraction
Diffraction patterns
tobacco
filaments
diffraction patterns
Tobacco
Diffraction
X-Rays
viruses
Viruses
X rays
Synchrotrons
graphene
alignment
diffraction
Scattering
X ray lasers

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Seuring, C., Ayyer, K., Filippaki, E., Barthelmess, M., Longchamp, J. N., Ringler, P., ... Chapman, H. N. (2018). Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background grapheme. Nature Communications, 9(1), [1836]. https://doi.org/10.1038/s41467-018-04116-9

Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background grapheme. / Seuring, Carolin; Ayyer, Kartik; Filippaki, Eleftheria; Barthelmess, Miriam; Longchamp, Jean Nicolas; Ringler, Philippe; Pardini, Tommaso; Wojtas, David H.; Coleman, Matthew A; Dörner, Katerina; Fuglerud, Silje; Hammarin, Greger; Habenstein, Birgit; Langkilde, Annette E.; Loquet, Antoine; Meents, Alke; Riek, Roland; Stahlberg, Henning; Boutet, Sébastien; Hunter, Mark S.; Koglin, Jason; Liang, Mengning; Ginn, Helen M.; Millane, Rick P.; Frank, Matthias; Barty, Anton; Chapman, Henry N.

In: Nature Communications, Vol. 9, No. 1, 1836, 01.12.2018.

Research output: Contribution to journal › Article

Seuring, C, Ayyer, K, Filippaki, E, Barthelmess, M, Longchamp, JN, Ringler, P, Pardini, T, Wojtas, DH, Coleman, MA, Dörner, K, Fuglerud, S, Hammarin, G, Habenstein, B, Langkilde, AE, Loquet, A, Meents, A, Riek, R, Stahlberg, H, Boutet, S, Hunter, MS, Koglin, J, Liang, M, Ginn, HM, Millane, RP, Frank, M, Barty, A & Chapman, HN 2018, 'Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background grapheme', Nature Communications, vol. 9, no. 1, 1836. https://doi.org/10.1038/s41467-018-04116-9
Seuring C, Ayyer K, Filippaki E, Barthelmess M, Longchamp JN, Ringler P et al. Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background grapheme. Nature Communications. 2018 Dec 1;9(1). 1836. https://doi.org/10.1038/s41467-018-04116-9
Seuring, Carolin ; Ayyer, Kartik ; Filippaki, Eleftheria ; Barthelmess, Miriam ; Longchamp, Jean Nicolas ; Ringler, Philippe ; Pardini, Tommaso ; Wojtas, David H. ; Coleman, Matthew A ; Dörner, Katerina ; Fuglerud, Silje ; Hammarin, Greger ; Habenstein, Birgit ; Langkilde, Annette E. ; Loquet, Antoine ; Meents, Alke ; Riek, Roland ; Stahlberg, Henning ; Boutet, Sébastien ; Hunter, Mark S. ; Koglin, Jason ; Liang, Mengning ; Ginn, Helen M. ; Millane, Rick P. ; Frank, Matthias ; Barty, Anton ; Chapman, Henry N. / Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background grapheme. In: Nature Communications. 2018 ; Vol. 9, No. 1.
@article{3d3a28ba2b8c459a8e688cf8f3b6d39b,
title = "Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background grapheme",
abstract = "Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 {\AA} and 2.4 {\AA} resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.",
author = "Carolin Seuring and Kartik Ayyer and Eleftheria Filippaki and Miriam Barthelmess and Longchamp, {Jean Nicolas} and Philippe Ringler and Tommaso Pardini and Wojtas, {David H.} and Coleman, {Matthew A} and Katerina D{\"o}rner and Silje Fuglerud and Greger Hammarin and Birgit Habenstein and Langkilde, {Annette E.} and Antoine Loquet and Alke Meents and Roland Riek and Henning Stahlberg and S{\'e}bastien Boutet and Hunter, {Mark S.} and Jason Koglin and Mengning Liang and Ginn, {Helen M.} and Millane, {Rick P.} and Matthias Frank and Anton Barty and Chapman, {Henry N.}",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41467-018-04116-9",
language = "English (US)",
volume = "9",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background grapheme

AU - Seuring, Carolin

AU - Ayyer, Kartik

AU - Filippaki, Eleftheria

AU - Barthelmess, Miriam

AU - Longchamp, Jean Nicolas

AU - Ringler, Philippe

AU - Pardini, Tommaso

AU - Wojtas, David H.

AU - Coleman, Matthew A

AU - Dörner, Katerina

AU - Fuglerud, Silje

AU - Hammarin, Greger

AU - Habenstein, Birgit

AU - Langkilde, Annette E.

AU - Loquet, Antoine

AU - Meents, Alke

AU - Riek, Roland

AU - Stahlberg, Henning

AU - Boutet, Sébastien

AU - Hunter, Mark S.

AU - Koglin, Jason

AU - Liang, Mengning

AU - Ginn, Helen M.

AU - Millane, Rick P.

AU - Frank, Matthias

AU - Barty, Anton

AU - Chapman, Henry N.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.

AB - Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.

UR - http://www.scopus.com/inward/record.url?scp=85046863333&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046863333&partnerID=8YFLogxK

U2 - 10.1038/s41467-018-04116-9

DO - 10.1038/s41467-018-04116-9

M3 - Article

VL - 9

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 1836

ER -

Access to Document