TY - JOUR
T1 - Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background grapheme
AU - Seuring, Carolin
AU - Ayyer, Kartik
AU - Filippaki, Eleftheria
AU - Barthelmess, Miriam
AU - Longchamp, Jean Nicolas
AU - Ringler, Philippe
AU - Pardini, Tommaso
AU - Wojtas, David H.
AU - Coleman, Matthew A
AU - Dörner, Katerina
AU - Fuglerud, Silje
AU - Hammarin, Greger
AU - Habenstein, Birgit
AU - Langkilde, Annette E.
AU - Loquet, Antoine
AU - Meents, Alke
AU - Riek, Roland
AU - Stahlberg, Henning
AU - Boutet, Sébastien
AU - Hunter, Mark S.
AU - Koglin, Jason
AU - Liang, Mengning
AU - Ginn, Helen M.
AU - Millane, Rick P.
AU - Frank, Matthias
AU - Barty, Anton
AU - Chapman, Henry N.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.
AB - Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.
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U2 - 10.1038/s41467-018-04116-9
DO - 10.1038/s41467-018-04116-9
M3 - Article
C2 - 29743480
AN - SCOPUS:85046863333
VL - 9
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 1836
ER -