Feline large granular lymphocyte (LGL) lymphoma with secondary leukemia

Primary intestinal origin with predominance of a CD3/CD8aa phenotype

P. Roccabianca, William Vernau, M. Caniatti, Peter F Moore

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Clinicopathologic and immunophenotypic characteristics of large granular lymphocyte (LGL) neoplasia in 21 cats were examined. All cats were domestic short (19) or long hair (2) with a mean age of 9.3 years at diagnosis. Increased peripheral blood LGL counts were present in 18/21 cats. Neutrophilia (12/21 cats) and increased serum liver enzymes (7/12), total and direct bilirubin (7/13), BUN (5/14), and creatinine (2/14) were observed. Cats usually presented with advanced disease and none survived longer than 84 days (mean 18.8 days) postdiagnosis. Cytologically, LGLs had a mature (6/21), immature (13/21), or mixed (2/21) morphology. Necropsy lesions consisted of neoplastic lymphoid infiltrates in the jejunum, ileum, and duodenum in decreasing order of frequency. In the small intestine, mucosal ulceration (9/13) and epitheliotropism of neoplastic cells (9/13) were common. Neoplastic infiltrates were also present in the mesenteric lymph nodes (13/13), liver (12/13), spleen (8/13), kidneys (5/7), and bone marrow (5/7). A T cell phenotype (CD3ε+) characterized LGL neoplasia in 19/21 cases. A CD8αα+ cytotoxic/suppressor phenotype was present in 12/19 T cell tumors, 2 had a CD4+CD8αα phenotype, 3 had a CD4-CD8- phenotype, and 2 were CD4+ helper T cells. CD8β chain expression was not detected in any instance. In two cats, a B or T cell origin could not be established. CD103 was expressed by 11 of 19 (58%) of the lymphomas tested. The immunophenotypic features shared by neoplastic LGLs in the cat and feline intestinal intraepithelial lymphocytes (IELs) support a small intestinal IEL origin for feline LGL lymphoma.

Original languageEnglish (US)
Pages (from-to)15-28
Number of pages14
JournalVeterinary Pathology
Volume43
Issue number1
DOIs
StatePublished - Jan 2006

Fingerprint

Felidae
lymphoma
leukemia
Lymphoma
Leukemia
Cats
lymphocytes
Lymphocytes
cats
Phenotype
phenotype
T-lymphocytes
T-Lymphocytes
neoplasms
Neoplasms
Liver
Blood Urea Nitrogen
Lymphocyte Count
Jejunum
Helper-Inducer T-Lymphocytes

Keywords

  • Cats
  • CD103 antigen
  • CD3 antigen
  • CD8 antigen
  • Immunohistochemistry
  • Intestines, small
  • Leukemia, large granular lymphocytic
  • Lymphocytes
  • Lymphoma, large granular lymphocytic

ASJC Scopus subject areas

  • veterinary(all)

Cite this

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title = "Feline large granular lymphocyte (LGL) lymphoma with secondary leukemia: Primary intestinal origin with predominance of a CD3/CD8aa phenotype",
abstract = "Clinicopathologic and immunophenotypic characteristics of large granular lymphocyte (LGL) neoplasia in 21 cats were examined. All cats were domestic short (19) or long hair (2) with a mean age of 9.3 years at diagnosis. Increased peripheral blood LGL counts were present in 18/21 cats. Neutrophilia (12/21 cats) and increased serum liver enzymes (7/12), total and direct bilirubin (7/13), BUN (5/14), and creatinine (2/14) were observed. Cats usually presented with advanced disease and none survived longer than 84 days (mean 18.8 days) postdiagnosis. Cytologically, LGLs had a mature (6/21), immature (13/21), or mixed (2/21) morphology. Necropsy lesions consisted of neoplastic lymphoid infiltrates in the jejunum, ileum, and duodenum in decreasing order of frequency. In the small intestine, mucosal ulceration (9/13) and epitheliotropism of neoplastic cells (9/13) were common. Neoplastic infiltrates were also present in the mesenteric lymph nodes (13/13), liver (12/13), spleen (8/13), kidneys (5/7), and bone marrow (5/7). A T cell phenotype (CD3ε+) characterized LGL neoplasia in 19/21 cases. A CD8αα+ cytotoxic/suppressor phenotype was present in 12/19 T cell tumors, 2 had a CD4+CD8αα phenotype, 3 had a CD4-CD8- phenotype, and 2 were CD4+ helper T cells. CD8β chain expression was not detected in any instance. In two cats, a B or T cell origin could not be established. CD103 was expressed by 11 of 19 (58{\%}) of the lymphomas tested. The immunophenotypic features shared by neoplastic LGLs in the cat and feline intestinal intraepithelial lymphocytes (IELs) support a small intestinal IEL origin for feline LGL lymphoma.",
keywords = "Cats, CD103 antigen, CD3 antigen, CD8 antigen, Immunohistochemistry, Intestines, small, Leukemia, large granular lymphocytic, Lymphocytes, Lymphoma, large granular lymphocytic",
author = "P. Roccabianca and William Vernau and M. Caniatti and Moore, {Peter F}",
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TY - JOUR

T1 - Feline large granular lymphocyte (LGL) lymphoma with secondary leukemia

T2 - Primary intestinal origin with predominance of a CD3/CD8aa phenotype

AU - Roccabianca, P.

AU - Vernau, William

AU - Caniatti, M.

AU - Moore, Peter F

PY - 2006/1

Y1 - 2006/1

N2 - Clinicopathologic and immunophenotypic characteristics of large granular lymphocyte (LGL) neoplasia in 21 cats were examined. All cats were domestic short (19) or long hair (2) with a mean age of 9.3 years at diagnosis. Increased peripheral blood LGL counts were present in 18/21 cats. Neutrophilia (12/21 cats) and increased serum liver enzymes (7/12), total and direct bilirubin (7/13), BUN (5/14), and creatinine (2/14) were observed. Cats usually presented with advanced disease and none survived longer than 84 days (mean 18.8 days) postdiagnosis. Cytologically, LGLs had a mature (6/21), immature (13/21), or mixed (2/21) morphology. Necropsy lesions consisted of neoplastic lymphoid infiltrates in the jejunum, ileum, and duodenum in decreasing order of frequency. In the small intestine, mucosal ulceration (9/13) and epitheliotropism of neoplastic cells (9/13) were common. Neoplastic infiltrates were also present in the mesenteric lymph nodes (13/13), liver (12/13), spleen (8/13), kidneys (5/7), and bone marrow (5/7). A T cell phenotype (CD3ε+) characterized LGL neoplasia in 19/21 cases. A CD8αα+ cytotoxic/suppressor phenotype was present in 12/19 T cell tumors, 2 had a CD4+CD8αα phenotype, 3 had a CD4-CD8- phenotype, and 2 were CD4+ helper T cells. CD8β chain expression was not detected in any instance. In two cats, a B or T cell origin could not be established. CD103 was expressed by 11 of 19 (58%) of the lymphomas tested. The immunophenotypic features shared by neoplastic LGLs in the cat and feline intestinal intraepithelial lymphocytes (IELs) support a small intestinal IEL origin for feline LGL lymphoma.

AB - Clinicopathologic and immunophenotypic characteristics of large granular lymphocyte (LGL) neoplasia in 21 cats were examined. All cats were domestic short (19) or long hair (2) with a mean age of 9.3 years at diagnosis. Increased peripheral blood LGL counts were present in 18/21 cats. Neutrophilia (12/21 cats) and increased serum liver enzymes (7/12), total and direct bilirubin (7/13), BUN (5/14), and creatinine (2/14) were observed. Cats usually presented with advanced disease and none survived longer than 84 days (mean 18.8 days) postdiagnosis. Cytologically, LGLs had a mature (6/21), immature (13/21), or mixed (2/21) morphology. Necropsy lesions consisted of neoplastic lymphoid infiltrates in the jejunum, ileum, and duodenum in decreasing order of frequency. In the small intestine, mucosal ulceration (9/13) and epitheliotropism of neoplastic cells (9/13) were common. Neoplastic infiltrates were also present in the mesenteric lymph nodes (13/13), liver (12/13), spleen (8/13), kidneys (5/7), and bone marrow (5/7). A T cell phenotype (CD3ε+) characterized LGL neoplasia in 19/21 cases. A CD8αα+ cytotoxic/suppressor phenotype was present in 12/19 T cell tumors, 2 had a CD4+CD8αα phenotype, 3 had a CD4-CD8- phenotype, and 2 were CD4+ helper T cells. CD8β chain expression was not detected in any instance. In two cats, a B or T cell origin could not be established. CD103 was expressed by 11 of 19 (58%) of the lymphomas tested. The immunophenotypic features shared by neoplastic LGLs in the cat and feline intestinal intraepithelial lymphocytes (IELs) support a small intestinal IEL origin for feline LGL lymphoma.

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KW - CD103 antigen

KW - CD3 antigen

KW - CD8 antigen

KW - Immunohistochemistry

KW - Intestines, small

KW - Leukemia, large granular lymphocytic

KW - Lymphocytes

KW - Lymphoma, large granular lymphocytic

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