Abstract
Feline immunodeficiency virus (FIV) gene orf-A, also designated orf-2, encodes a 77 amino acid accessory protein reported to be critical for efficient viral replication in vitro and in vivo and previously implicated to encode a Tat protein for FIV. However, recent studies have shown Orf-A to be important in the late steps of the FIV life cycle involved in virion formation and in early steps involved in virus infectivity (J. Virol. 77 (2003) 8819). The present study reports that expression of a GFP-Orf-A fusion protein in both primate and feline cell lines results in nuclear localization of this FIV accessory protein. Moreover, a nuclear localization signal (NLS) critical for nuclear import was mapped to amino acid residues 43 through 53 of Orf-A. Lastly, transient expression of GFP-Orf-A in cells induced an arrest at the second gap (G 2) of the cell cycle. Our findings reveal that Orf-A is a nuclear protein that expresses properties similar to those reported for human immunodeficiency virus-1 (HIV-1)-encoded Vpr.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 167-174 |
| Number of pages | 8 |
| Journal | Virology |
| Volume | 325 |
| Issue number | 2 |
| DOIs | |
| State | Published - Aug 1 2004 |
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Keywords
- Amino acid
- FIV
- HIV
ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
Feline immunodeficiency virus Orf-A localizes to the nucleus and induces cell cycle arrest. / Gemeniano, Malou C.; Sawai, Earl T.; Sparger, Ellen E.
In: Virology, Vol. 325, No. 2, 01.08.2004, p. 167-174.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Feline immunodeficiency virus Orf-A localizes to the nucleus and induces cell cycle arrest
AU - Gemeniano, Malou C.
AU - Sawai, Earl T.
AU - Sparger, Ellen E.
PY - 2004/8/1
Y1 - 2004/8/1
N2 - Feline immunodeficiency virus (FIV) gene orf-A, also designated orf-2, encodes a 77 amino acid accessory protein reported to be critical for efficient viral replication in vitro and in vivo and previously implicated to encode a Tat protein for FIV. However, recent studies have shown Orf-A to be important in the late steps of the FIV life cycle involved in virion formation and in early steps involved in virus infectivity (J. Virol. 77 (2003) 8819). The present study reports that expression of a GFP-Orf-A fusion protein in both primate and feline cell lines results in nuclear localization of this FIV accessory protein. Moreover, a nuclear localization signal (NLS) critical for nuclear import was mapped to amino acid residues 43 through 53 of Orf-A. Lastly, transient expression of GFP-Orf-A in cells induced an arrest at the second gap (G 2) of the cell cycle. Our findings reveal that Orf-A is a nuclear protein that expresses properties similar to those reported for human immunodeficiency virus-1 (HIV-1)-encoded Vpr.
AB - Feline immunodeficiency virus (FIV) gene orf-A, also designated orf-2, encodes a 77 amino acid accessory protein reported to be critical for efficient viral replication in vitro and in vivo and previously implicated to encode a Tat protein for FIV. However, recent studies have shown Orf-A to be important in the late steps of the FIV life cycle involved in virion formation and in early steps involved in virus infectivity (J. Virol. 77 (2003) 8819). The present study reports that expression of a GFP-Orf-A fusion protein in both primate and feline cell lines results in nuclear localization of this FIV accessory protein. Moreover, a nuclear localization signal (NLS) critical for nuclear import was mapped to amino acid residues 43 through 53 of Orf-A. Lastly, transient expression of GFP-Orf-A in cells induced an arrest at the second gap (G 2) of the cell cycle. Our findings reveal that Orf-A is a nuclear protein that expresses properties similar to those reported for human immunodeficiency virus-1 (HIV-1)-encoded Vpr.
KW - Amino acid
KW - FIV
KW - HIV
UR - http://www.scopus.com/inward/record.url?scp=3142568692&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3142568692&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2004.05.007
DO - 10.1016/j.virol.2004.05.007
M3 - Article
C2 - 15246256
AN - SCOPUS:3142568692
VL - 325
SP - 167
EP - 174
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -