Feline immunodeficiency virus Orf-A localizes to the nucleus and induces cell cycle arrest

Malou C. Gemeniano, Earl T. Sawai, Ellen E. Sparger

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Feline immunodeficiency virus (FIV) gene orf-A, also designated orf-2, encodes a 77 amino acid accessory protein reported to be critical for efficient viral replication in vitro and in vivo and previously implicated to encode a Tat protein for FIV. However, recent studies have shown Orf-A to be important in the late steps of the FIV life cycle involved in virion formation and in early steps involved in virus infectivity (J. Virol. 77 (2003) 8819). The present study reports that expression of a GFP-Orf-A fusion protein in both primate and feline cell lines results in nuclear localization of this FIV accessory protein. Moreover, a nuclear localization signal (NLS) critical for nuclear import was mapped to amino acid residues 43 through 53 of Orf-A. Lastly, transient expression of GFP-Orf-A in cells induced an arrest at the second gap (G 2) of the cell cycle. Our findings reveal that Orf-A is a nuclear protein that expresses properties similar to those reported for human immunodeficiency virus-1 (HIV-1)-encoded Vpr.

Original languageEnglish (US)
Pages (from-to)167-174
Number of pages8
JournalVirology
Volume325
Issue number2
DOIs
StatePublished - Aug 1 2004

Fingerprint

Contagious Ecthyma
Feline Immunodeficiency Virus
Cell Cycle Checkpoints
tat Gene Products
Amino Acids
Nuclear Localization Signals
Gastrin-Secreting Cells
Proteins
Cell Nucleus Active Transport
Felidae
Nuclear Proteins
Life Cycle Stages
Virion
Primates
HIV-1
Cell Cycle
Viruses
Cell Line

Keywords

  • Amino acid
  • FIV
  • HIV

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Feline immunodeficiency virus Orf-A localizes to the nucleus and induces cell cycle arrest. / Gemeniano, Malou C.; Sawai, Earl T.; Sparger, Ellen E.

In: Virology, Vol. 325, No. 2, 01.08.2004, p. 167-174.

Research output: Contribution to journalArticle

Gemeniano, Malou C. ; Sawai, Earl T. ; Sparger, Ellen E. / Feline immunodeficiency virus Orf-A localizes to the nucleus and induces cell cycle arrest. In: Virology. 2004 ; Vol. 325, No. 2. pp. 167-174.
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