Fecal shedding of Mycobacterium avium subsp. paratuberculosis by dairy cows

Beate Crossley, Francisco J. Zagmutt-Vergara, Terry L. Fyock, Robert H. Whitlock, Ian Gardner

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Between 1982 and 2000, fecal samples were obtained from 786 cows that were shedding Mycobacterium avium subsp. paratuberculosis (Map). These cows were resident on 93 Pennsylvania dairies (mean herd size, 64 milk cows) that had no or minimal previous testing for Map. Feces were cultured on four tubes of Herrold's egg yolk medium and the distribution of mean Map colony forming units (CFU) was evaluated. Most cows were light (<10 CFU/tube, 51.4%) or high (>50 CFU/tube, 30.8%) fecal shedders with fewer cows in the moderate category (10-50 CFU/tube). Of the 786 cows, 192 (24.4%) had colonies in only one of four tubes. In the multivariable negative binomial model, there were significant associations between mean CFU/tube and prevalence, herd size, and season and an interaction between herd size and season. The linear mixed model of continuous tube counts with a random herd effect yielded similar findings with associations with herd size as a continuous variable, season, and an interaction between categorized prevalence and continuous herd size. Variability in CFU/tube was greatest among cows in the same herd, intermediate for replicate tubes from the same cow, and smallest among cows in different herds. Reduction in the number of replicate tubes from four would have reduced the sensitivity of fecal culture for Map by approximately 6% (for three tubes) to 12% (for two tubes).

Original languageEnglish (US)
Pages (from-to)257-263
Number of pages7
JournalVeterinary Microbiology
Volume107
Issue number3-4
DOIs
StatePublished - May 20 2005

Fingerprint

Paratuberculosis
Mycobacterium avium
Mycobacterium avium subsp. paratuberculosis
Stem Cells
dairy cows
herd size
cows
herds
Egg Yolk
Statistical Models
Feces
Linear Models
Milk
Light
egg yolk
dairy herds
feces

Keywords

  • Cattle
  • Colony forming units
  • Feces
  • Isolation
  • Mycobacterium paratuberculosis

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

Fecal shedding of Mycobacterium avium subsp. paratuberculosis by dairy cows. / Crossley, Beate; Zagmutt-Vergara, Francisco J.; Fyock, Terry L.; Whitlock, Robert H.; Gardner, Ian.

In: Veterinary Microbiology, Vol. 107, No. 3-4, 20.05.2005, p. 257-263.

Research output: Contribution to journalArticle

Crossley, Beate ; Zagmutt-Vergara, Francisco J. ; Fyock, Terry L. ; Whitlock, Robert H. ; Gardner, Ian. / Fecal shedding of Mycobacterium avium subsp. paratuberculosis by dairy cows. In: Veterinary Microbiology. 2005 ; Vol. 107, No. 3-4. pp. 257-263.
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