Fatty acid desaturase regulation in adipose tissue by dietary composition is independent of weight loss and is correlated with the plasma triacylglycerol response

Lara M. Mangravite, Kevin Dawson, Ryan R. Davis, Jeffrey Gregg, Ronald M. Krauss

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background: Atherogenic dyslipidemia associated with elevated plasma triacylglycerol concentrations and reduced HDL is improved by both caloric restriction and reduced carbohydrate consumption. Objective: We aimed to identify the molecular pathways responsive to both caloric restriction and dietary composition within adipose tissue by monitoring transcriptional expression. Design: Subcutaneous adipose tissue biopsy specimens were obtained from 131 moderately overweight men [body mass index (in kg/m2): 29.2 ± 2.0] after 1 wk of a basal diet [54%, 16%, and 30% carbohydrate, protein, and fat, respectively; 7% saturated fat), after 3 wk with 1 of 4 randomized diets [basal diet; moderate-carbohydrate (39%) diet; low-carbohydrate (26%) and low-saturated-fat (9%) diet; low-carbohydrate (26%) and high-saturated-fat (15%) diet], after 5 wk of acute weight loss with a randomized diet (-1103.0 ± 216.5 kcal/d, which resulted in a loss of 10.0 ± 3.3 lb, or 4.5 ± 1.5 kg), and after 4 wk of being stabilized at a reduced weight. Transcriptional response was identified by using expression array analysis and was confirmed by using real-time polymerase chain reaction analysis. Results: Of the 1473 transcripts significantly decreased in expression in response to acute weight loss, 30 were responsive to isocaloric alterations in dietary composition, including stearoyl-coenzyme A desaturase (SCD), fatty acid desaturases 1 and 2 (FADS1 and FADS2), and diacylglycerol transferase 2 (DGAT2). Response was confirmed by real-time polymerase chain reaction analysis for these genes (P < 0.003). SCD expression in response to isocaloric dietary change was moststrongly correlated with carbohydrate intake (P=0.019) and, with the low-carbohydrate diet, SCD expression was inversely correlated with saturated fat intake (P = 0.05). Triacylglycerol responses to changes in dietary composition were independently correlated with SCD (P = 0.003) and DGAT2 (P = 0.05) responses. Conclusions: SCD expression in adipose tissue is independently regulated by weight loss and by carbohydrate and saturated fat intakes. Moreover, SCD and DGAT2 expression may be involved in dietary regulation of systemic triacylglycerol metabolism.

Original languageEnglish (US)
Pages (from-to)759-767
Number of pages9
JournalAmerican Journal of Clinical Nutrition
Volume86
Issue number3
StatePublished - Sep 1 2007

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Fatty Acid Desaturases
stearoyl-CoA desaturase
coenzyme A
adipose tissue
Adipose Tissue
Weight Loss
Triglycerides
weight loss
triacylglycerols
Carbohydrates
Diet
Fats
carbohydrates
diet
Caloric Restriction
fat intake
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
Carbohydrate-Restricted Diet
low carbohydrate diet

Keywords

  • Adipose tissue
  • Dietary carbohydrate
  • Dietary saturated fat
  • Fatty acid desaturases
  • Gene expression
  • Gene expression microarray
  • Gene transcription
  • Triacylglycerol
  • Weight loss

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Fatty acid desaturase regulation in adipose tissue by dietary composition is independent of weight loss and is correlated with the plasma triacylglycerol response. / Mangravite, Lara M.; Dawson, Kevin; Davis, Ryan R.; Gregg, Jeffrey; Krauss, Ronald M.

In: American Journal of Clinical Nutrition, Vol. 86, No. 3, 01.09.2007, p. 759-767.

Research output: Contribution to journalArticle

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abstract = "Background: Atherogenic dyslipidemia associated with elevated plasma triacylglycerol concentrations and reduced HDL is improved by both caloric restriction and reduced carbohydrate consumption. Objective: We aimed to identify the molecular pathways responsive to both caloric restriction and dietary composition within adipose tissue by monitoring transcriptional expression. Design: Subcutaneous adipose tissue biopsy specimens were obtained from 131 moderately overweight men [body mass index (in kg/m2): 29.2 ± 2.0] after 1 wk of a basal diet [54{\%}, 16{\%}, and 30{\%} carbohydrate, protein, and fat, respectively; 7{\%} saturated fat), after 3 wk with 1 of 4 randomized diets [basal diet; moderate-carbohydrate (39{\%}) diet; low-carbohydrate (26{\%}) and low-saturated-fat (9{\%}) diet; low-carbohydrate (26{\%}) and high-saturated-fat (15{\%}) diet], after 5 wk of acute weight loss with a randomized diet (-1103.0 ± 216.5 kcal/d, which resulted in a loss of 10.0 ± 3.3 lb, or 4.5 ± 1.5 kg), and after 4 wk of being stabilized at a reduced weight. Transcriptional response was identified by using expression array analysis and was confirmed by using real-time polymerase chain reaction analysis. Results: Of the 1473 transcripts significantly decreased in expression in response to acute weight loss, 30 were responsive to isocaloric alterations in dietary composition, including stearoyl-coenzyme A desaturase (SCD), fatty acid desaturases 1 and 2 (FADS1 and FADS2), and diacylglycerol transferase 2 (DGAT2). Response was confirmed by real-time polymerase chain reaction analysis for these genes (P < 0.003). SCD expression in response to isocaloric dietary change was moststrongly correlated with carbohydrate intake (P=0.019) and, with the low-carbohydrate diet, SCD expression was inversely correlated with saturated fat intake (P = 0.05). Triacylglycerol responses to changes in dietary composition were independently correlated with SCD (P = 0.003) and DGAT2 (P = 0.05) responses. Conclusions: SCD expression in adipose tissue is independently regulated by weight loss and by carbohydrate and saturated fat intakes. Moreover, SCD and DGAT2 expression may be involved in dietary regulation of systemic triacylglycerol metabolism.",
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T1 - Fatty acid desaturase regulation in adipose tissue by dietary composition is independent of weight loss and is correlated with the plasma triacylglycerol response

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AU - Dawson, Kevin

AU - Davis, Ryan R.

AU - Gregg, Jeffrey

AU - Krauss, Ronald M.

PY - 2007/9/1

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N2 - Background: Atherogenic dyslipidemia associated with elevated plasma triacylglycerol concentrations and reduced HDL is improved by both caloric restriction and reduced carbohydrate consumption. Objective: We aimed to identify the molecular pathways responsive to both caloric restriction and dietary composition within adipose tissue by monitoring transcriptional expression. Design: Subcutaneous adipose tissue biopsy specimens were obtained from 131 moderately overweight men [body mass index (in kg/m2): 29.2 ± 2.0] after 1 wk of a basal diet [54%, 16%, and 30% carbohydrate, protein, and fat, respectively; 7% saturated fat), after 3 wk with 1 of 4 randomized diets [basal diet; moderate-carbohydrate (39%) diet; low-carbohydrate (26%) and low-saturated-fat (9%) diet; low-carbohydrate (26%) and high-saturated-fat (15%) diet], after 5 wk of acute weight loss with a randomized diet (-1103.0 ± 216.5 kcal/d, which resulted in a loss of 10.0 ± 3.3 lb, or 4.5 ± 1.5 kg), and after 4 wk of being stabilized at a reduced weight. Transcriptional response was identified by using expression array analysis and was confirmed by using real-time polymerase chain reaction analysis. Results: Of the 1473 transcripts significantly decreased in expression in response to acute weight loss, 30 were responsive to isocaloric alterations in dietary composition, including stearoyl-coenzyme A desaturase (SCD), fatty acid desaturases 1 and 2 (FADS1 and FADS2), and diacylglycerol transferase 2 (DGAT2). Response was confirmed by real-time polymerase chain reaction analysis for these genes (P < 0.003). SCD expression in response to isocaloric dietary change was moststrongly correlated with carbohydrate intake (P=0.019) and, with the low-carbohydrate diet, SCD expression was inversely correlated with saturated fat intake (P = 0.05). Triacylglycerol responses to changes in dietary composition were independently correlated with SCD (P = 0.003) and DGAT2 (P = 0.05) responses. Conclusions: SCD expression in adipose tissue is independently regulated by weight loss and by carbohydrate and saturated fat intakes. Moreover, SCD and DGAT2 expression may be involved in dietary regulation of systemic triacylglycerol metabolism.

AB - Background: Atherogenic dyslipidemia associated with elevated plasma triacylglycerol concentrations and reduced HDL is improved by both caloric restriction and reduced carbohydrate consumption. Objective: We aimed to identify the molecular pathways responsive to both caloric restriction and dietary composition within adipose tissue by monitoring transcriptional expression. Design: Subcutaneous adipose tissue biopsy specimens were obtained from 131 moderately overweight men [body mass index (in kg/m2): 29.2 ± 2.0] after 1 wk of a basal diet [54%, 16%, and 30% carbohydrate, protein, and fat, respectively; 7% saturated fat), after 3 wk with 1 of 4 randomized diets [basal diet; moderate-carbohydrate (39%) diet; low-carbohydrate (26%) and low-saturated-fat (9%) diet; low-carbohydrate (26%) and high-saturated-fat (15%) diet], after 5 wk of acute weight loss with a randomized diet (-1103.0 ± 216.5 kcal/d, which resulted in a loss of 10.0 ± 3.3 lb, or 4.5 ± 1.5 kg), and after 4 wk of being stabilized at a reduced weight. Transcriptional response was identified by using expression array analysis and was confirmed by using real-time polymerase chain reaction analysis. Results: Of the 1473 transcripts significantly decreased in expression in response to acute weight loss, 30 were responsive to isocaloric alterations in dietary composition, including stearoyl-coenzyme A desaturase (SCD), fatty acid desaturases 1 and 2 (FADS1 and FADS2), and diacylglycerol transferase 2 (DGAT2). Response was confirmed by real-time polymerase chain reaction analysis for these genes (P < 0.003). SCD expression in response to isocaloric dietary change was moststrongly correlated with carbohydrate intake (P=0.019) and, with the low-carbohydrate diet, SCD expression was inversely correlated with saturated fat intake (P = 0.05). Triacylglycerol responses to changes in dietary composition were independently correlated with SCD (P = 0.003) and DGAT2 (P = 0.05) responses. Conclusions: SCD expression in adipose tissue is independently regulated by weight loss and by carbohydrate and saturated fat intakes. Moreover, SCD and DGAT2 expression may be involved in dietary regulation of systemic triacylglycerol metabolism.

KW - Adipose tissue

KW - Dietary carbohydrate

KW - Dietary saturated fat

KW - Fatty acid desaturases

KW - Gene expression

KW - Gene expression microarray

KW - Gene transcription

KW - Triacylglycerol

KW - Weight loss

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