Fate of the atrioventricular endocardial cushions in the developing chick heart

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

To determine the fate of the atrioventricular endocardial cushions in cardiac development, we used staining methods for extracellular fibronectin, which is abundant in the endocardial cushions, and actin, which is abundant in the myocytes. White Leghorn chick embryo hearts were harvested at Hamburger and Hamilton stages 26 to 36, and serial sections of the atrioventricular valve region were stained. Before atrioventricular valve formation, fibronectin and actin staining reveal separation between the fibronectin-rich endocardial cushions and the actin-rich myocardial layer. The developing mitral valve leaflets at all of the observed stages contain a fibronectin-rich matrix but no actin-rich myocytes. In contrast, the tricuspid band includes both fibronectin matrix and actin-rich cells. We conclude that the mitral valve leaflets in the chick form predominantly from the endocardial cushion tissue, and the tricuspid band receives contributions from both the endocardial cushions and surrounding myocardium.

Original languageEnglish (US)
Pages (from-to)390-393
Number of pages4
JournalPediatric Research
Volume32
Issue number4
StatePublished - 1992

Fingerprint

Endocardial Cushions
Fibronectins
Actins
Mitral Valve
Muscle Cells
Staining and Labeling
Chick Embryo
Myocardium

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Fate of the atrioventricular endocardial cushions in the developing chick heart. / Chin, C.; Gandour-Edwards, Regina F; Oltjen, S.; Choy, Michael.

In: Pediatric Research, Vol. 32, No. 4, 1992, p. 390-393.

Research output: Contribution to journalArticle

@article{89d663a7cf444cd19765e7f113b6dac9,
title = "Fate of the atrioventricular endocardial cushions in the developing chick heart",
abstract = "To determine the fate of the atrioventricular endocardial cushions in cardiac development, we used staining methods for extracellular fibronectin, which is abundant in the endocardial cushions, and actin, which is abundant in the myocytes. White Leghorn chick embryo hearts were harvested at Hamburger and Hamilton stages 26 to 36, and serial sections of the atrioventricular valve region were stained. Before atrioventricular valve formation, fibronectin and actin staining reveal separation between the fibronectin-rich endocardial cushions and the actin-rich myocardial layer. The developing mitral valve leaflets at all of the observed stages contain a fibronectin-rich matrix but no actin-rich myocytes. In contrast, the tricuspid band includes both fibronectin matrix and actin-rich cells. We conclude that the mitral valve leaflets in the chick form predominantly from the endocardial cushion tissue, and the tricuspid band receives contributions from both the endocardial cushions and surrounding myocardium.",
author = "C. Chin and Gandour-Edwards, {Regina F} and S. Oltjen and Michael Choy",
year = "1992",
language = "English (US)",
volume = "32",
pages = "390--393",
journal = "Pediatric Research",
issn = "0031-3998",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Fate of the atrioventricular endocardial cushions in the developing chick heart

AU - Chin, C.

AU - Gandour-Edwards, Regina F

AU - Oltjen, S.

AU - Choy, Michael

PY - 1992

Y1 - 1992

N2 - To determine the fate of the atrioventricular endocardial cushions in cardiac development, we used staining methods for extracellular fibronectin, which is abundant in the endocardial cushions, and actin, which is abundant in the myocytes. White Leghorn chick embryo hearts were harvested at Hamburger and Hamilton stages 26 to 36, and serial sections of the atrioventricular valve region were stained. Before atrioventricular valve formation, fibronectin and actin staining reveal separation between the fibronectin-rich endocardial cushions and the actin-rich myocardial layer. The developing mitral valve leaflets at all of the observed stages contain a fibronectin-rich matrix but no actin-rich myocytes. In contrast, the tricuspid band includes both fibronectin matrix and actin-rich cells. We conclude that the mitral valve leaflets in the chick form predominantly from the endocardial cushion tissue, and the tricuspid band receives contributions from both the endocardial cushions and surrounding myocardium.

AB - To determine the fate of the atrioventricular endocardial cushions in cardiac development, we used staining methods for extracellular fibronectin, which is abundant in the endocardial cushions, and actin, which is abundant in the myocytes. White Leghorn chick embryo hearts were harvested at Hamburger and Hamilton stages 26 to 36, and serial sections of the atrioventricular valve region were stained. Before atrioventricular valve formation, fibronectin and actin staining reveal separation between the fibronectin-rich endocardial cushions and the actin-rich myocardial layer. The developing mitral valve leaflets at all of the observed stages contain a fibronectin-rich matrix but no actin-rich myocytes. In contrast, the tricuspid band includes both fibronectin matrix and actin-rich cells. We conclude that the mitral valve leaflets in the chick form predominantly from the endocardial cushion tissue, and the tricuspid band receives contributions from both the endocardial cushions and surrounding myocardium.

UR - http://www.scopus.com/inward/record.url?scp=0026700771&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026700771&partnerID=8YFLogxK

M3 - Article

C2 - 1437388

AN - SCOPUS:0026700771

VL - 32

SP - 390

EP - 393

JO - Pediatric Research

JF - Pediatric Research

SN - 0031-3998

IS - 4

ER -