Fatal Septicemia Caused by the Zoonotic Bacterium Streptococcus iniae During an Outbreak in Caribbean Reef Fish

N. D. Keirstead, J. W. Brake, M. J. Griffin, I. Halliday-Simmonds, M. A. Thrall, Esteban Soto Martinez

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

An outbreak of Streptococcus iniae occurred in the early months of 2008 among wild reef fish in the waters of the Federation of St Kitts and Nevis, lasting almost 2 months. Moribund and dead fish were collected for gross, histological, bacteriological, and molecular analysis. Necropsy findings included diffuse fibrinous pericarditis, pale friable livers, and serosal petechiation. Cytological and histological analysis revealed granulocytic and granulomatous inflammation with abundant coccoid bacterial organisms forming long chains. Necrosis, inflammation, and vasculitis were most severe in the pericardium, meninges, liver, kidneys, and gills. Bacterial isolates revealed β-hemolytic, Gram-positive coccoid bacteria identified as S. iniae by amplification and 16S ribosomal RNA gene sequencing. Results from biochemical and antimicrobial susceptibility analysis, together with repetitive element palindromic polymerase chain reaction fingerprinting, suggest that a single strain was responsible for the outbreak. The inciting cause for this S. iniae-associated cluster of mortalities is unknown.

Original languageEnglish (US)
Pages (from-to)1035-1041
Number of pages7
JournalVeterinary Pathology
Volume51
Issue number5
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Streptococcus iniae
Zoonoses
septicemia
Disease Outbreaks
reefs
Sepsis
Fishes
Bacteria
Saint Kitts and Nevis
bacteria
inflammation
fish
16S Ribosomal RNA
Inflammation
RNA Sequence Analysis
meninges
pericarditis
pericardium
Meninges
vasculitis

Keywords

  • outbreak
  • reef fish
  • septicemia
  • Streptococcus iniae
  • zoonotic

ASJC Scopus subject areas

  • veterinary(all)
  • Medicine(all)

Cite this

Fatal Septicemia Caused by the Zoonotic Bacterium Streptococcus iniae During an Outbreak in Caribbean Reef Fish. / Keirstead, N. D.; Brake, J. W.; Griffin, M. J.; Halliday-Simmonds, I.; Thrall, M. A.; Soto Martinez, Esteban.

In: Veterinary Pathology, Vol. 51, No. 5, 2014, p. 1035-1041.

Research output: Contribution to journalArticle

Keirstead, N. D. ; Brake, J. W. ; Griffin, M. J. ; Halliday-Simmonds, I. ; Thrall, M. A. ; Soto Martinez, Esteban. / Fatal Septicemia Caused by the Zoonotic Bacterium Streptococcus iniae During an Outbreak in Caribbean Reef Fish. In: Veterinary Pathology. 2014 ; Vol. 51, No. 5. pp. 1035-1041.
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