Fast and precise detection of ricin with microcapillary sensor system

Jun Tae Lee, Dosi Dosev, Mikaela Nichkova, Zhiya Ma, Shirley Gee, Bruce D. Hammock, Ian M. Kennedy

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Ricin is an easily available toxin which can be used as a bio-terror agent. Fast and inexpensive methods for its detection in different samples are needed. Recently we have developed a novel fluorescent sandwich immunoassay for ricin using magnetic-luminescent nanoparticles (MLNPs) as carriers in a microcapillary system for incubation and detection. Antiricin antibody coated MLNPs that were dispersed in buffer solution were introduced in the capillary tube and immobilized inside using an external electromagnet. Then the sample containing ricin was injected while the MLNPs were mixed by an alternating magnetic field. After the incubation, washing solution and secondary antibody conjugated with Alexa-fluorescent were injected into the capillary while the MLNPs were constantly mixed. After the final wash, the particles were immobilized for detection. The total analysis time was reduced to less than forty minutes which is about 8-10 fold improvement in comparison with the plate-based protocols. This system is promising for the development of a portable biosensor and can be used for the detection of other analytes of interest.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7207
DOIs
StatePublished - 2009
EventMicrofluidics, BioMEMS, and Medical Microsystems VII - San Jose, CA, United States
Duration: Jan 26 2009Jan 28 2009

Other

OtherMicrofluidics, BioMEMS, and Medical Microsystems VII
CountryUnited States
CitySan Jose, CA
Period1/26/091/28/09

Fingerprint

Ricin
Nanoparticles
nanoparticles
sensors
Sensors
antibodies
Antibodies
Capillary tubes
immunoassay
capillary tubes
Electromagnets
electromagnets
Magnets
washing
Biosensing Techniques
Magnetic Fields
Immunoassay
bioinstrumentation
Biosensors
Washing

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Lee, J. T., Dosev, D., Nichkova, M., Ma, Z., Gee, S., Hammock, B. D., & Kennedy, I. M. (2009). Fast and precise detection of ricin with microcapillary sensor system. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7207). [72070G] https://doi.org/10.1117/12.809421

Fast and precise detection of ricin with microcapillary sensor system. / Lee, Jun Tae; Dosev, Dosi; Nichkova, Mikaela; Ma, Zhiya; Gee, Shirley; Hammock, Bruce D.; Kennedy, Ian M.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7207 2009. 72070G.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lee, JT, Dosev, D, Nichkova, M, Ma, Z, Gee, S, Hammock, BD & Kennedy, IM 2009, Fast and precise detection of ricin with microcapillary sensor system. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7207, 72070G, Microfluidics, BioMEMS, and Medical Microsystems VII, San Jose, CA, United States, 1/26/09. https://doi.org/10.1117/12.809421
Lee JT, Dosev D, Nichkova M, Ma Z, Gee S, Hammock BD et al. Fast and precise detection of ricin with microcapillary sensor system. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7207. 2009. 72070G https://doi.org/10.1117/12.809421
Lee, Jun Tae ; Dosev, Dosi ; Nichkova, Mikaela ; Ma, Zhiya ; Gee, Shirley ; Hammock, Bruce D. ; Kennedy, Ian M. / Fast and precise detection of ricin with microcapillary sensor system. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7207 2009.
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