Factors influencing rat survival in a warm renal ischemia model

Time to adapt the protocols

R. N. Raman, C. D. Pivetti, R. Ramsamooj, Dennis L Matthews, S. G. Demos, Christoph Troppmann

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Introduction: Survival in warm renal ischemia models is not only dependent on the treatment or surgical technique being evaluated, but also on factors inherent to the model itself. Use of rats of various strains in previous studies makes interstudy comparison difficult when trying to design an appropriate model control that would yield intermediate survival. In this study, impact of rat strain on survival after prolonged warm renal ischemia in the setting of delivery-controlled inhalational anesthesia was evaluated. Materials and methods: Under general delivery-controlled inhalation anesthesia with isoflurane, Dahl salt-sensitive, Wistar-Furth, Sprague-Dawley, and spontaneously hypertensive rats (n = 66 rats) were subjected to 150 minutes of unilateral renal warm ischemia time, subsequent reperfusion, and contralateral nephrectomy. Animals were followed up for 1 month, after which survivors were euthanized and morphologic changes in kidneys were scored. Results: Thirty-day survival was: Dahl salt sensitive, 78%; Wistar-Furth, 67%; Sprague-Dawley, 55%; and spontaneously hypertensive rats, 0% (P < .0001). Histologic acute injury scores were higher for non-survivors versus 30-day survivors (P < .0001). Conclusion: Our data strongly suggest that rat strain is a major factor influencing survival and that strain and warm ischemia time selections must be considered together when designing a model control yielding intermediate survival. Further study is warranted in order to compare the effect of delivery-controlled inhalational versus historical anesthesia methods on animal survival.

Original languageEnglish (US)
Pages (from-to)1511-1514
Number of pages4
JournalTransplantation Proceedings
Volume43
Issue number5
DOIs
StatePublished - Jun 2011

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Warm Ischemia
Kidney
Inbred SHR Rats
Anesthesia
Salts
Inhalation Anesthesia
Isoflurane
Nephrectomy
Reperfusion
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

Factors influencing rat survival in a warm renal ischemia model : Time to adapt the protocols. / Raman, R. N.; Pivetti, C. D.; Ramsamooj, R.; Matthews, Dennis L; Demos, S. G.; Troppmann, Christoph.

In: Transplantation Proceedings, Vol. 43, No. 5, 06.2011, p. 1511-1514.

Research output: Contribution to journalArticle

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