Facilitation of TRPV4 by TRPV1 is required for itch transmission in some sensory neuron populations

Seungil Kim, Devin M. Barry, Xian Yu Liu, Shijin Yin, Admire Munanairi, Qing Tao Meng, Wei Cheng, Ping Mo, Li Wan, Shen Bin Liu, Kasun Ratnayake, Zhong Qiu Zhao, Narasimhan Gautam, Jie Zheng, W. K Ajith Karunarathne, Zhou Feng Chen

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The transient receptor potential channels (TRPs) respond to chemical irritants and temperature. TRPV1 responds to the itch-inducing endogenous signal histamine, and TRPA1 responds to the itch-inducing chemical chloroquine. We showed that, in sensory neurons, TRPV4 is important for both chloroquineand histamine-induced itch and that TRPV1 has a role in chloroquine-induced itch. Chloroquine-induced scratching was reduced in mice in which TRPV1 was knocked down or pharmacologically inhibited. Both TRPV4 and TRPV1 were present in some sensory neurons. Pharmacological blockade of either TRPV4 or TRPV1 significantly attenuated the Ca2+ response of sensory neurons exposed to histamine or chloroquine. Knockout of Trpv1 impaired Ca2+ responses and reduced scratching behavior evoked by a TRPV4 agonist, whereas knockout of Trpv4 did not alter TRPV1-mediated capsaicin responses. Electrophysiological analysis of human embryonic kidney (HEK) 293 cells coexpressing TRPV4 and TRPV1 revealed that the presence of both channels enhanced the activation kinetics of TRPV4 but not of TRPV1. Biochemical and biophysical studies suggested a close proximity between TRPV4 and TRPV1 in dorsal root ganglion neurons and in cultured cells. Thus, our studies identified TRPV4 as a channel that contributes to both histamine- and chloroquine-induced itch and indicated that the function of TRPV4 in itch signaling involves TRPV1-mediated facilitation. TRP facilitation through the formation of heteromeric complexes could be a prevalent mechanism by which the vast array of somatosensory information is encoded in sensory neurons.

Original languageEnglish (US)
Article numberra71
JournalScience Signaling
Volume9
Issue number437
DOIs
StatePublished - Jul 19 2016

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Chloroquine
Sensory Receptor Cells
Neurons
Histamine
Population
Transient Receptor Potential Channels
Irritants
Capsaicin
Spinal Ganglia
Cultured Cells
Chemical activation
Cells
Pharmacology
Kidney
Kinetics
Temperature

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Kim, S., Barry, D. M., Liu, X. Y., Yin, S., Munanairi, A., Meng, Q. T., ... Chen, Z. F. (2016). Facilitation of TRPV4 by TRPV1 is required for itch transmission in some sensory neuron populations. Science Signaling, 9(437), [ra71]. https://doi.org/10.1126/scisignal.aaf1047

Facilitation of TRPV4 by TRPV1 is required for itch transmission in some sensory neuron populations. / Kim, Seungil; Barry, Devin M.; Liu, Xian Yu; Yin, Shijin; Munanairi, Admire; Meng, Qing Tao; Cheng, Wei; Mo, Ping; Wan, Li; Liu, Shen Bin; Ratnayake, Kasun; Zhao, Zhong Qiu; Gautam, Narasimhan; Zheng, Jie; Karunarathne, W. K Ajith; Chen, Zhou Feng.

In: Science Signaling, Vol. 9, No. 437, ra71, 19.07.2016.

Research output: Contribution to journalArticle

Kim, S, Barry, DM, Liu, XY, Yin, S, Munanairi, A, Meng, QT, Cheng, W, Mo, P, Wan, L, Liu, SB, Ratnayake, K, Zhao, ZQ, Gautam, N, Zheng, J, Karunarathne, WKA & Chen, ZF 2016, 'Facilitation of TRPV4 by TRPV1 is required for itch transmission in some sensory neuron populations', Science Signaling, vol. 9, no. 437, ra71. https://doi.org/10.1126/scisignal.aaf1047
Kim, Seungil ; Barry, Devin M. ; Liu, Xian Yu ; Yin, Shijin ; Munanairi, Admire ; Meng, Qing Tao ; Cheng, Wei ; Mo, Ping ; Wan, Li ; Liu, Shen Bin ; Ratnayake, Kasun ; Zhao, Zhong Qiu ; Gautam, Narasimhan ; Zheng, Jie ; Karunarathne, W. K Ajith ; Chen, Zhou Feng. / Facilitation of TRPV4 by TRPV1 is required for itch transmission in some sensory neuron populations. In: Science Signaling. 2016 ; Vol. 9, No. 437.
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abstract = "The transient receptor potential channels (TRPs) respond to chemical irritants and temperature. TRPV1 responds to the itch-inducing endogenous signal histamine, and TRPA1 responds to the itch-inducing chemical chloroquine. We showed that, in sensory neurons, TRPV4 is important for both chloroquineand histamine-induced itch and that TRPV1 has a role in chloroquine-induced itch. Chloroquine-induced scratching was reduced in mice in which TRPV1 was knocked down or pharmacologically inhibited. Both TRPV4 and TRPV1 were present in some sensory neurons. Pharmacological blockade of either TRPV4 or TRPV1 significantly attenuated the Ca2+ response of sensory neurons exposed to histamine or chloroquine. Knockout of Trpv1 impaired Ca2+ responses and reduced scratching behavior evoked by a TRPV4 agonist, whereas knockout of Trpv4 did not alter TRPV1-mediated capsaicin responses. Electrophysiological analysis of human embryonic kidney (HEK) 293 cells coexpressing TRPV4 and TRPV1 revealed that the presence of both channels enhanced the activation kinetics of TRPV4 but not of TRPV1. Biochemical and biophysical studies suggested a close proximity between TRPV4 and TRPV1 in dorsal root ganglion neurons and in cultured cells. Thus, our studies identified TRPV4 as a channel that contributes to both histamine- and chloroquine-induced itch and indicated that the function of TRPV4 in itch signaling involves TRPV1-mediated facilitation. TRP facilitation through the formation of heteromeric complexes could be a prevalent mechanism by which the vast array of somatosensory information is encoded in sensory neurons.",
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AU - Wan, Li

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AU - Zhao, Zhong Qiu

AU - Gautam, Narasimhan

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AU - Chen, Zhou Feng

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