Facile generation of cell microarrays using vacuum degassing and coverslip sweeping

Min S. Wang, Zhen Luo, Sundar Cherukuri, Nitin Nitin

Research output: Contribution to journalArticle

Abstract

A simple method to generate cell microarrays with high-percentage well occupancy and well-defined cell confinement is presented. This method uses a synergistic combination of vacuum degassing and coverslip sweeping. The vacuum degassing step dislodges air bubbles from the microwells, which in turn enables the cells to enter the microwells, while the physical sweeping step using a glass coverslip removes the excess cells outside the microwells. This low-cost preparation method provides a simple solution to generating cell microarrays that can be performed in basic research laboratories and point-of-care settings for routine cell-based screening assays.

Original languageEnglish (US)
Pages (from-to)48-50
Number of pages3
JournalAnalytical Biochemistry
Volume457
DOIs
StatePublished - Jul 15 2014

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Degassing
Microarrays
Vacuum
Research laboratories
Assays
Screening
Glass
Air
Point-of-Care Systems
Costs
Costs and Cost Analysis
Research

Keywords

  • Cells
  • Drug response
  • Fluorescence microscopy
  • Microwell array
  • Screening

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Medicine(all)

Cite this

Facile generation of cell microarrays using vacuum degassing and coverslip sweeping. / Wang, Min S.; Luo, Zhen; Cherukuri, Sundar; Nitin, Nitin.

In: Analytical Biochemistry, Vol. 457, 15.07.2014, p. 48-50.

Research output: Contribution to journalArticle

Wang, Min S. ; Luo, Zhen ; Cherukuri, Sundar ; Nitin, Nitin. / Facile generation of cell microarrays using vacuum degassing and coverslip sweeping. In: Analytical Biochemistry. 2014 ; Vol. 457. pp. 48-50.
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