Facile chemoenzymatic synthesis of biotinylated heparosan hexasaccharide

Baolin Wu; Na Wei; Vireak Thon; Mohui Wei; Zaikuan Yu; Yongmei Xu; Xi Chen; Jian Liu; Peng George Wang; Tiehai Li

doi:10.1039/c5ob00462d

Facile chemoenzymatic synthesis of biotinylated heparosan hexasaccharide

Baolin Wu, Na Wei, Vireak Thon, Mohui Wei, Zaikuan Yu, Yongmei Xu, Xi Chen, Jian Liu, Peng George Wang, Tiehai Li

Chemistry

Research output: Contribution to journal › Article

9 Scopus citations

Abstract

A biotinylated heparosan hexasaccharide was synthesized using a one-pot multi-enzyme strategy, in situ activation and transfer of N-trifluoroacetylglucosamine (GlcNTFA) to a heparin backbone significantly improved the synthetic efficiency. The biotinylated hexasaccharide could serve as a flexible core to diversify its conversion into heparan sulfate isoforms with potential biological applications and therapeutics.

Original language	English (US)
Pages (from-to)	5098-5101
Number of pages	4
Journal	Organic and Biomolecular Chemistry
Volume	13
Issue number	18
DOIs	https://doi.org/10.1039/c5ob00462d
State	Published - May 14 2015

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ASJC Scopus subject areas

Physical and Theoretical Chemistry
Organic Chemistry
Biochemistry

Cite this

Wu, B., Wei, N., Thon, V., Wei, M., Yu, Z., Xu, Y., Chen, X., Liu, J., Wang, P. G., & Li, T. (2015). Facile chemoenzymatic synthesis of biotinylated heparosan hexasaccharide. Organic and Biomolecular Chemistry, 13(18), 5098-5101. https://doi.org/10.1039/c5ob00462d

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AU - Wei, Na

AU - Thon, Vireak

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AU - Yu, Zaikuan

AU - Xu, Yongmei

AU - Chen, Xi

AU - Liu, Jian

AU - Wang, Peng George

AU - Li, Tiehai

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Access to Document

10.1039/c5ob00462d