Facile chemoenzymatic synthesis of biotinylated heparosan hexasaccharide

Baolin Wu; Na Wei; Vireak Thon; Mohui Wei; Zaikuan Yu; Yongmei Xu; Xi Chen; Jian Liu; Peng George Wang; Tiehai Li

doi:10.1039/c5ob00462d

Facile chemoenzymatic synthesis of biotinylated heparosan hexasaccharide

Baolin Wu, Na Wei, Vireak Thon, Mohui Wei, Zaikuan Yu, Yongmei Xu, Xi Chen, Jian Liu, Peng George Wang, Tiehai Li

Chemistry

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

A biotinylated heparosan hexasaccharide was synthesized using a one-pot multi-enzyme strategy, in situ activation and transfer of N-trifluoroacetylglucosamine (GlcNTFA) to a heparin backbone significantly improved the synthetic efficiency. The biotinylated hexasaccharide could serve as a flexible core to diversify its conversion into heparan sulfate isoforms with potential biological applications and therapeutics.

Original language	English (US)
Pages (from-to)	5098-5101
Number of pages	4
Journal	Organic and Biomolecular Chemistry
Volume	13
Issue number	18
DOIs	https://doi.org/10.1039/c5ob00462d
State	Published - May 14 2015

ASJC Scopus subject areas

Physical and Theoretical Chemistry
Organic Chemistry
Biochemistry

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title = "Facile chemoenzymatic synthesis of biotinylated heparosan hexasaccharide",

abstract = "A biotinylated heparosan hexasaccharide was synthesized using a one-pot multi-enzyme strategy, in situ activation and transfer of N-trifluoroacetylglucosamine (GlcNTFA) to a heparin backbone significantly improved the synthetic efficiency. The biotinylated hexasaccharide could serve as a flexible core to diversify its conversion into heparan sulfate isoforms with potential biological applications and therapeutics.",

author = "Baolin Wu and Na Wei and Vireak Thon and Mohui Wei and Zaikuan Yu and Yongmei Xu and Xi Chen and Jian Liu and Wang, {Peng George} and Tiehai Li",

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T1 - Facile chemoenzymatic synthesis of biotinylated heparosan hexasaccharide

AU - Wu, Baolin

AU - Wei, Na

AU - Thon, Vireak

AU - Wei, Mohui

AU - Yu, Zaikuan

AU - Xu, Yongmei

AU - Chen, Xi

AU - Liu, Jian

AU - Wang, Peng George

AU - Li, Tiehai

PY - 2015/5/14

Y1 - 2015/5/14

N2 - A biotinylated heparosan hexasaccharide was synthesized using a one-pot multi-enzyme strategy, in situ activation and transfer of N-trifluoroacetylglucosamine (GlcNTFA) to a heparin backbone significantly improved the synthetic efficiency. The biotinylated hexasaccharide could serve as a flexible core to diversify its conversion into heparan sulfate isoforms with potential biological applications and therapeutics.

AB - A biotinylated heparosan hexasaccharide was synthesized using a one-pot multi-enzyme strategy, in situ activation and transfer of N-trifluoroacetylglucosamine (GlcNTFA) to a heparin backbone significantly improved the synthetic efficiency. The biotinylated hexasaccharide could serve as a flexible core to diversify its conversion into heparan sulfate isoforms with potential biological applications and therapeutics.

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U2 - 10.1039/c5ob00462d

DO - 10.1039/c5ob00462d

M3 - Article

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JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

SN - 1477-0520

IS - 18

ER -

Facile chemoenzymatic synthesis of biotinylated heparosan hexasaccharide

Abstract

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