Facile chemoenzymatic synthesis of biotinylated heparosan hexasaccharide

Baolin Wu, Na Wei, Vireak Thon, Mohui Wei, Zaikuan Yu, Yongmei Xu, Xi Chen, Jian Liu, Peng George Wang, Tiehai Li

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A biotinylated heparosan hexasaccharide was synthesized using a one-pot multi-enzyme strategy, in situ activation and transfer of N-trifluoroacetylglucosamine (GlcNTFA) to a heparin backbone significantly improved the synthetic efficiency. The biotinylated hexasaccharide could serve as a flexible core to diversify its conversion into heparan sulfate isoforms with potential biological applications and therapeutics.

Original languageEnglish (US)
Pages (from-to)5098-5101
Number of pages4
JournalOrganic and Biomolecular Chemistry
Volume13
Issue number18
DOIs
StatePublished - May 14 2015

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heparins
Heparitin Sulfate
Heparin
enzymes
sulfates
Protein Isoforms
Chemical activation
activation
Enzymes
synthesis
Therapeutics
heparosan

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Biochemistry

Cite this

Wu, B., Wei, N., Thon, V., Wei, M., Yu, Z., Xu, Y., ... Li, T. (2015). Facile chemoenzymatic synthesis of biotinylated heparosan hexasaccharide. Organic and Biomolecular Chemistry, 13(18), 5098-5101. https://doi.org/10.1039/c5ob00462d

Facile chemoenzymatic synthesis of biotinylated heparosan hexasaccharide. / Wu, Baolin; Wei, Na; Thon, Vireak; Wei, Mohui; Yu, Zaikuan; Xu, Yongmei; Chen, Xi; Liu, Jian; Wang, Peng George; Li, Tiehai.

In: Organic and Biomolecular Chemistry, Vol. 13, No. 18, 14.05.2015, p. 5098-5101.

Research output: Contribution to journalArticle

Wu, B, Wei, N, Thon, V, Wei, M, Yu, Z, Xu, Y, Chen, X, Liu, J, Wang, PG & Li, T 2015, 'Facile chemoenzymatic synthesis of biotinylated heparosan hexasaccharide', Organic and Biomolecular Chemistry, vol. 13, no. 18, pp. 5098-5101. https://doi.org/10.1039/c5ob00462d
Wu, Baolin ; Wei, Na ; Thon, Vireak ; Wei, Mohui ; Yu, Zaikuan ; Xu, Yongmei ; Chen, Xi ; Liu, Jian ; Wang, Peng George ; Li, Tiehai. / Facile chemoenzymatic synthesis of biotinylated heparosan hexasaccharide. In: Organic and Biomolecular Chemistry. 2015 ; Vol. 13, No. 18. pp. 5098-5101.
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