Fabrication of poly(ethylene glycol) hydrogel microstructures using photolithography

Alexander Revzin, Ryan J. Russell, Vamsi K. Yadavalli, Won Gun Koh, Curt Deister, David D. Hile, Michael B. Mellott, Michael V. Pishko

Research output: Contribution to journalArticle

386 Citations (Scopus)

Abstract

The fabrication of hydrogel microstructures based upon poly(ethylene glycol) diacrylates, dimethacrylates, and tetraacrylates patterned photolithographically on silicon or glass substrates is described. A silicon/silicon dioxide surface was treated with 3-(trichlorosilyl)propyl methacrylate to form a self-assembled monolayer (SAM) with pendant acrylate groups. The SAM presence on the surface was verified using ellipsometry and time-of-flight secondary ion mass spectrometry. A solution containing an acrylated or methacrylated poly(ethylene glycol) derivative and a photoinitiator (2,2-dimethoxy-2-phenylacetophenone) was spin-coated onto the treated substrate, exposed to 365 nm ultraviolet light through a photomask, and developed with either toluene, water, or supercritical CO2. As a result of this process, three-dimensional, cross-linked PEG hydrogel microstructures were immobilized on the surface. Diameters of cylindrical array members were varied from 600 to 7 μm by the use of different photomasks, while height varied from 3 to 12 μm, depending on the molecular weight of the PEG macromer. In the case of μm diameter elements, as many as 400 elements were reproducibly generated in a 1 mm2 square pattern. The resultant hydrogel patterns were hydrated for as long as 3 weeks without delamination from the substrate. In addition, micropatterning of different molecular weights of PEG was demonstrated. Arrays of hydrogel disks containing an immobilized protein conjugated to a pH sensitive fluorophore were also prepared. The pH sensitivity of the gel-immobilized dye was similar to that in an aqueous buffer, and no leaching of the dye-labeled protein from the hydrogel microstructure was observed over a 1 week period. Changes in fluorescence were also observed for immobilized fluorophore labeled acetylcholine esterase upon the addition of acetyl acholine.

Original languageEnglish (US)
Pages (from-to)5440-5447
Number of pages8
JournalLangmuir
Volume17
Issue number18
DOIs
StatePublished - Sep 4 2001
Externally publishedYes

Fingerprint

Hydrogel
Photolithography
photolithography
Hydrogels
Polyethylene glycols
glycols
ethylene
photomasks
Fabrication
microstructure
Microstructure
fabrication
molecular weight
dyes
Photomasks
Fluorophores
acetylcholine
proteins
Self assembled monolayers
Silicon

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Revzin, A., Russell, R. J., Yadavalli, V. K., Koh, W. G., Deister, C., Hile, D. D., ... Pishko, M. V. (2001). Fabrication of poly(ethylene glycol) hydrogel microstructures using photolithography. Langmuir, 17(18), 5440-5447. https://doi.org/10.1021/la010075w

Fabrication of poly(ethylene glycol) hydrogel microstructures using photolithography. / Revzin, Alexander; Russell, Ryan J.; Yadavalli, Vamsi K.; Koh, Won Gun; Deister, Curt; Hile, David D.; Mellott, Michael B.; Pishko, Michael V.

In: Langmuir, Vol. 17, No. 18, 04.09.2001, p. 5440-5447.

Research output: Contribution to journalArticle

Revzin, A, Russell, RJ, Yadavalli, VK, Koh, WG, Deister, C, Hile, DD, Mellott, MB & Pishko, MV 2001, 'Fabrication of poly(ethylene glycol) hydrogel microstructures using photolithography', Langmuir, vol. 17, no. 18, pp. 5440-5447. https://doi.org/10.1021/la010075w
Revzin A, Russell RJ, Yadavalli VK, Koh WG, Deister C, Hile DD et al. Fabrication of poly(ethylene glycol) hydrogel microstructures using photolithography. Langmuir. 2001 Sep 4;17(18):5440-5447. https://doi.org/10.1021/la010075w
Revzin, Alexander ; Russell, Ryan J. ; Yadavalli, Vamsi K. ; Koh, Won Gun ; Deister, Curt ; Hile, David D. ; Mellott, Michael B. ; Pishko, Michael V. / Fabrication of poly(ethylene glycol) hydrogel microstructures using photolithography. In: Langmuir. 2001 ; Vol. 17, No. 18. pp. 5440-5447.
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