Fabrication of photo-crosslinkable poly (Trimethylene carbonate)/polycaprolactone nanofibrous scaffolds for tendon regeneration

Xing Li, Honglin Chen, Shuting Xie, Ning Wang, Sujuan Wu, Yuyou Duan, Minmin Zhang, Lingling Shui

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Background: The treatment of tendon injuries remains a challenging problem in clinical due to their slow and insufficient natural healing process. Scaffold-based tissue engineering provides a promising strategy to facilitate tendon healing and regeneration. However, many tissue engineering scaffolds have failed due to their poor and unstable mechanical properties. To address this, we fabricated nanofibrous polycaprolactone/methacrylated poly(trimethylene carbonate) (PCL/PTMC-MA) composite scaffolds via electrospinning. Materials and Methods: PTMC-MA was characterized by nuclear magnetic resonance. Fiber morphology of composite scaffolds was evaluated using scanning electron microscopy. The monotonic tensile test was performed for determining the mechanical properties of composite scaffolds. Cell viability and collagen deposition were assessed via PrestoBlue assay and enzyme-linked immunosorbent assay, respectively. Results: These PCL/PTMC-MA composite scaffolds had an increase in mechanical properties as PTMC-MA content increase. After photo-crosslinking, they showed further enhanced mechanical properties including creep resistance, which was superior to pure PCL scaffolds. It is worth noting that photo-crosslinked PCL/PTMC-MA (1:3) composite scaffolds had a Young’s modulus of 31.13 ± 1.30 MPa and Max stress at break of 23.80 ± 3.44 MPa that were comparable with the mechanical properties of native tendon (Young’s modulus 20–1200 MPa, max stress at break 5–100 MPa). In addition, biological experiments demonstrated that PCL/PTMC-MA composite scaffolds were biocompatible for cell adhesion, proliferation, and differentiation.

Original languageEnglish (US)
Pages (from-to)6373-6383
Number of pages11
JournalInternational journal of nanomedicine
Volume15
DOIs
StatePublished - 2020
Externally publishedYes

Keywords

  • Composite scaffolds
  • Creep resistance
  • Photo-crosslinking
  • Poly(trimethylene carbonate)
  • Tissue engineering

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

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