Fabrication of an inexpensive, implantable cooling device for reversible brain deactivation in animals ranging from rodents to primates

Dylan F. Cooke, Adam B. Goldring, Itsukyo Yamayoshi, Phillippos Tsourkas, Gregg H. Recanzone, Alex Tiriac, Tingrui Pan, Scott I. Simon, Leah Krubitzer

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have developed a compact and lightweight micro fluidic cooling device to reversibly deactivate one or more areas of the neo cortex to examine its functional macrocircuitry as well as behavioral and cortical plasticity. The device, which we term the "cooling chip," consists of thin silicone tubing (through which chilled ethanol is circulated) embedded in mechanically compliant polydimethylsiloxane (PDMS). PDMS is tailored to compact device dimensions (as small as 21 mm3) that precisely accommodate the geometry of the targeted cortical area. The biocompatible design makes it suitable for both acute preparations and chronic implantation for long-term behavioral studies. The cooling chip accommodates an in-cortex microthermocouple measuring local cortical temperature. A microelectrode may be used to record simultaneous neural responses at the same location. Cortex temperature is controlled by computer regulation of the coolant flow, which can achieve a localized cortical temperature drop from 37 to 20°C in less than 3 min and maintain target temperature to within ±0.3°C indefinitely. Here we describe cooling chip fabrication and performance in mediating cessation of neural signaling in acute preparations of rodents, ferrets, and primates.

Original languageEnglish (US)
Pages (from-to)3543-3558
Number of pages16
JournalJournal of Neurophysiology
Volume107
Issue number12
DOIs
StatePublished - Jun 15 2012

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Primates
Rodentia
Equipment and Supplies
Temperature
Brain
Ferrets
Microelectrodes
Silicones
Ethanol
baysilon

Keywords

  • Biocompatible
  • Cortex
  • Cryoloop
  • Inactivation
  • Magnetic resonance compatible
  • Plasticity

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Cooke, D. F., Goldring, A. B., Yamayoshi, I., Tsourkas, P., Recanzone, G. H., Tiriac, A., ... Krubitzer, L. (2012). Fabrication of an inexpensive, implantable cooling device for reversible brain deactivation in animals ranging from rodents to primates. Journal of Neurophysiology, 107(12), 3543-3558. https://doi.org/10.1152/jn.01101.2011

Fabrication of an inexpensive, implantable cooling device for reversible brain deactivation in animals ranging from rodents to primates. / Cooke, Dylan F.; Goldring, Adam B.; Yamayoshi, Itsukyo; Tsourkas, Phillippos; Recanzone, Gregg H.; Tiriac, Alex; Pan, Tingrui; Simon, Scott I.; Krubitzer, Leah.

In: Journal of Neurophysiology, Vol. 107, No. 12, 15.06.2012, p. 3543-3558.

Research output: Contribution to journalArticle

Cooke, DF, Goldring, AB, Yamayoshi, I, Tsourkas, P, Recanzone, GH, Tiriac, A, Pan, T, Simon, SI & Krubitzer, L 2012, 'Fabrication of an inexpensive, implantable cooling device for reversible brain deactivation in animals ranging from rodents to primates', Journal of Neurophysiology, vol. 107, no. 12, pp. 3543-3558. https://doi.org/10.1152/jn.01101.2011
Cooke, Dylan F. ; Goldring, Adam B. ; Yamayoshi, Itsukyo ; Tsourkas, Phillippos ; Recanzone, Gregg H. ; Tiriac, Alex ; Pan, Tingrui ; Simon, Scott I. ; Krubitzer, Leah. / Fabrication of an inexpensive, implantable cooling device for reversible brain deactivation in animals ranging from rodents to primates. In: Journal of Neurophysiology. 2012 ; Vol. 107, No. 12. pp. 3543-3558.
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