Fabrication and imaging of nanometer-sized protein patterns

Kapila Wadu-Mesthrige, Song Xu, Nabil A. Amro, Gang-yu Liu

Research output: Contribution to journalArticlepeer-review

173 Scopus citations


This Letter describes a new method for production of nanometer-sized protein patterns with precise control of the size and geometry. Self-assembled monolayers (SAMs) are first patterned using nanografting, an atomic force microscopy (AFM)-based lithographic method. Methyl-terminated n-alkanethiols serve as matrix layers within which nanopatterns of thiols with carboxylate or aldehyde terminal groups are fabricated. By controlling solution conditions, we selectively immobilize proteins on the patterned areas, through either electrostatic interactions or covalent binding. In our approach, AFM tips are used for both fabrication and characterization. AFM allows patterned SAMs and proteins to be visualized in situ. The individual proteins within the nanopatterns and their orientations can be clearly resolved from the AFM images.

Original languageEnglish (US)
Pages (from-to)8580-8583
Number of pages4
Issue number25
StatePublished - Dec 7 1999
Externally publishedYes

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry


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