Abstract
Porous multi-channel chitosan conduits were fabricated using a novel phase-separation technique with an axial temperature gradient. First, porous chitosan tubes were made with a mold that was composed of two concentric polytetrafiuoroethylene tubes. Then 1%-3% (w/v) chitosan solution was injected into the chitosan tube while the two ends of the tube were closed with steel rods. Then the outside of the tube was wrapped with a layer of thermal insulating material to reduce the heat transfer through the outside, and the tubes were placed in a freezer. The resulting phase separation then occurred in the presence of an axial temperature gradient. The porosity, microtubule diameter, and orientation were controlled by adjusting the polymer concentration and temperature gradient. After the preparation course, no poisonous substances remained on the conduits. The mechanical properties, swelling, and biodegradability of the chitosan conduits were investigated, and a scanning electron microscope was used to observe the tubular morphology and growth of neuroblastoma cells (N2A, mouse) in the conduits. The results demonstrate that the multi-channel chitosan conduits have suitable mechanical strength, swelling, degradation properties, and nerve cell affinity, so they hold promise for use as neural tissue engineering scaffolds.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 435-438 |
| Number of pages | 4 |
| Journal | Tsinghua Science and Technology |
| Volume | 10 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 2005 |
| Externally published | Yes |
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Keywords
- Chitosan
- Nerve conduits
- Phase separation
- Tissue engineering
ASJC Scopus subject areas
- General
Cite this
Fabrication and characterization of chitosan nerve conduits with microtubular architectures. / Ao, Qiang; Wang, Aijun; Cao, Wenling; Zhao, Chang; Gong, Yandao; Zhao, Nanming; Zhang, Xiufang.
In: Tsinghua Science and Technology, Vol. 10, No. 4, 08.2005, p. 435-438.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fabrication and characterization of chitosan nerve conduits with microtubular architectures
AU - Ao, Qiang
AU - Wang, Aijun
AU - Cao, Wenling
AU - Zhao, Chang
AU - Gong, Yandao
AU - Zhao, Nanming
AU - Zhang, Xiufang
PY - 2005/8
Y1 - 2005/8
N2 - Porous multi-channel chitosan conduits were fabricated using a novel phase-separation technique with an axial temperature gradient. First, porous chitosan tubes were made with a mold that was composed of two concentric polytetrafiuoroethylene tubes. Then 1%-3% (w/v) chitosan solution was injected into the chitosan tube while the two ends of the tube were closed with steel rods. Then the outside of the tube was wrapped with a layer of thermal insulating material to reduce the heat transfer through the outside, and the tubes were placed in a freezer. The resulting phase separation then occurred in the presence of an axial temperature gradient. The porosity, microtubule diameter, and orientation were controlled by adjusting the polymer concentration and temperature gradient. After the preparation course, no poisonous substances remained on the conduits. The mechanical properties, swelling, and biodegradability of the chitosan conduits were investigated, and a scanning electron microscope was used to observe the tubular morphology and growth of neuroblastoma cells (N2A, mouse) in the conduits. The results demonstrate that the multi-channel chitosan conduits have suitable mechanical strength, swelling, degradation properties, and nerve cell affinity, so they hold promise for use as neural tissue engineering scaffolds.
AB - Porous multi-channel chitosan conduits were fabricated using a novel phase-separation technique with an axial temperature gradient. First, porous chitosan tubes were made with a mold that was composed of two concentric polytetrafiuoroethylene tubes. Then 1%-3% (w/v) chitosan solution was injected into the chitosan tube while the two ends of the tube were closed with steel rods. Then the outside of the tube was wrapped with a layer of thermal insulating material to reduce the heat transfer through the outside, and the tubes were placed in a freezer. The resulting phase separation then occurred in the presence of an axial temperature gradient. The porosity, microtubule diameter, and orientation were controlled by adjusting the polymer concentration and temperature gradient. After the preparation course, no poisonous substances remained on the conduits. The mechanical properties, swelling, and biodegradability of the chitosan conduits were investigated, and a scanning electron microscope was used to observe the tubular morphology and growth of neuroblastoma cells (N2A, mouse) in the conduits. The results demonstrate that the multi-channel chitosan conduits have suitable mechanical strength, swelling, degradation properties, and nerve cell affinity, so they hold promise for use as neural tissue engineering scaffolds.
KW - Chitosan
KW - Nerve conduits
KW - Phase separation
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=24044513293&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=24044513293&partnerID=8YFLogxK
U2 - 10.1016/S1007-0214(05)70096-1
DO - 10.1016/S1007-0214(05)70096-1
M3 - Article
AN - SCOPUS:24044513293
VL - 10
SP - 435
EP - 438
JO - Tsinghua Science and Technology
JF - Tsinghua Science and Technology
SN - 1007-0214
IS - 4
ER -