Eye Opener in Stroke

Hung Nguyen, Jea Young Lee, Paul R. Sanberg, Eleonora Napoli, Cesar V. Borlongan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background and Purpose- Retinal ischemia is a major cause of visual impairment in stroke patients, but our incomplete understanding of its pathology may contribute to a lack of effective treatment. Here, we investigated the role of mitochondrial dysfunction in retinal ischemia and probed the potential of mesenchymal stem cells (MSCs) in mitochondrial repair under such pathological condition. Methods- In vivo, rats were subjected to middle cerebral artery occlusion then randomly treated with intravenous MSCs or vehicle. Laser Doppler was used to evaluate the blood flow in the brain and the eye, while immunohistochemical staining assessed cellular degeneration at days 3 and 14 poststroke. In vitro, retinal pigmented epithelium cells were exposed to either oxygen-glucose deprivation or oxygen-glucose deprivation and coculture with MSCs, and subsequently, cell death and mitochondrial function were examined immunocytochemically and with Seahorse analyzer, respectively. Results- Middle cerebral artery occlusion significantly reduced blood flow in the brain and the eye accompanied by mitochondrial dysfunction and ganglion cell death at days 3 and 14 poststroke. Intravenous MSCs elicited mitochondrial repair and improved ganglion cell survival at day 14 poststroke. Oxygen-glucose deprivation similarly induced mitochondrial dysfunction and cell death in retinal pigmented epithelium cells; coculture with MSCs restored mitochondrial respiration, mitochondrial network morphology, and mitochondrial dynamics, which likely attenuated oxygen-glucose deprivation-mediated retinal pigmented epithelium cell death. Conclusions- Retinal ischemia is closely associated with mitochondrial dysfunction, which can be remedied by stem cell-mediated mitochondrial repair.

Original languageEnglish (US)
Pages (from-to)2197-2206
Number of pages10
JournalStroke
Volume50
Issue number8
DOIs
StatePublished - Aug 1 2019

Fingerprint

Mesenchymal Stromal Cells
Stroke
Cell Death
Oxygen
Glucose
Ischemia
Epithelium
Middle Cerebral Artery Infarction
Coculture Techniques
Ganglia
Mitochondrial Dynamics
Smegmamorpha
Vision Disorders
Brain
Cell Survival
Respiration
Lasers
Stem Cells
Pathology
Staining and Labeling

Keywords

  • cell survival
  • endothelial cells
  • glucose
  • mitochondrial dynamics
  • oxygen

ASJC Scopus subject areas

  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine
  • Advanced and Specialized Nursing

Cite this

Nguyen, H., Lee, J. Y., Sanberg, P. R., Napoli, E., & Borlongan, C. V. (2019). Eye Opener in Stroke. Stroke, 50(8), 2197-2206. https://doi.org/10.1161/STROKEAHA.119.025249

Eye Opener in Stroke. / Nguyen, Hung; Lee, Jea Young; Sanberg, Paul R.; Napoli, Eleonora; Borlongan, Cesar V.

In: Stroke, Vol. 50, No. 8, 01.08.2019, p. 2197-2206.

Research output: Contribution to journalArticle

Nguyen, H, Lee, JY, Sanberg, PR, Napoli, E & Borlongan, CV 2019, 'Eye Opener in Stroke', Stroke, vol. 50, no. 8, pp. 2197-2206. https://doi.org/10.1161/STROKEAHA.119.025249
Nguyen H, Lee JY, Sanberg PR, Napoli E, Borlongan CV. Eye Opener in Stroke. Stroke. 2019 Aug 1;50(8):2197-2206. https://doi.org/10.1161/STROKEAHA.119.025249
Nguyen, Hung ; Lee, Jea Young ; Sanberg, Paul R. ; Napoli, Eleonora ; Borlongan, Cesar V. / Eye Opener in Stroke. In: Stroke. 2019 ; Vol. 50, No. 8. pp. 2197-2206.
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