Extrinsic Incubation Rate is not Accelerated in Recent California Strains of West Nile Virus in Culex tarsalis (Diptera: Culicidae)

Mary E. Danforth, William Reisen, Chris Barker

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The efficiency of West Nile virus (WNV) transmission by competent mosquito vectors is driven by temperature and defined, in part, by the extrinsic incubation period, which is the time from a mosquito's consumption of an infected bloodmeal until it becomes capable of transmitting the virus to the next vertebrate host. The extrinsic incubation period can be altered by a variety of factors involved in vector-pathogen interactions, and in North America, the WN02 strain of WNV emerged and displaced the founding NY99 strain reportedly because the duration of the extrinsic incubation period in Culex mosquitoes was shortened by a single positively selected mutation. However, recent work has suggested that this change is not universal and may depend on vector species or strain. In the current study, we estimated the extrinsic incubation periods at 22 and 30°C in Culex tarsalis Coquillett. We found that the time to transmission of the original North American WNV strain, NY99, was not different from two more recent California isolates of the WN02 genotype: one of the earliest California isolates from the southeastern deserts, and a more recent 2011 isolate from a hyperendemic region in the Central Valley. We conclude with a model-based assessment of the epidemiological effects of temperature on the duration of mosquitoes' infectious life, which estimated that most mosquitoes have an infectious life of only a few days, but its duration expands markedly at warmer temperatures.

Original languageEnglish (US)
Pages (from-to)1083-1089
Number of pages7
JournalJournal of Medical Entomology
Volume52
Issue number5
DOIs
StatePublished - Jan 1 2015

Fingerprint

Culex tarsalis
West Nile virus
Culex
Culicidae
Diptera
Temperature
duration
North America
temperature
virus transmission
Vertebrates
Genotype
deserts
Viruses
valleys
Mutation
vertebrates
mutation
viruses
pathogens

Keywords

  • Culex tarsalis
  • incubation period
  • temperature
  • transmission
  • West Nile virus

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

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title = "Extrinsic Incubation Rate is not Accelerated in Recent California Strains of West Nile Virus in Culex tarsalis (Diptera: Culicidae)",
abstract = "The efficiency of West Nile virus (WNV) transmission by competent mosquito vectors is driven by temperature and defined, in part, by the extrinsic incubation period, which is the time from a mosquito's consumption of an infected bloodmeal until it becomes capable of transmitting the virus to the next vertebrate host. The extrinsic incubation period can be altered by a variety of factors involved in vector-pathogen interactions, and in North America, the WN02 strain of WNV emerged and displaced the founding NY99 strain reportedly because the duration of the extrinsic incubation period in Culex mosquitoes was shortened by a single positively selected mutation. However, recent work has suggested that this change is not universal and may depend on vector species or strain. In the current study, we estimated the extrinsic incubation periods at 22 and 30°C in Culex tarsalis Coquillett. We found that the time to transmission of the original North American WNV strain, NY99, was not different from two more recent California isolates of the WN02 genotype: one of the earliest California isolates from the southeastern deserts, and a more recent 2011 isolate from a hyperendemic region in the Central Valley. We conclude with a model-based assessment of the epidemiological effects of temperature on the duration of mosquitoes' infectious life, which estimated that most mosquitoes have an infectious life of only a few days, but its duration expands markedly at warmer temperatures.",
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AU - Barker, Chris

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