Extraordinary phylogenetic diversity and metabolic versatility in aquifer sediment

Cindy J. Castelle, Laura A. Hug, Kelly C. Wrighton, Brian C. Thomas, Kenneth H. Williams, Dongying Wu, Susannah G. Tringe, Steven W. Singer, Jonathan A Eisen, Jillian F. Banfield

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Microorganisms in the subsurface represent a substantial but poorly understood component of the Earth's biosphere. Subsurface environments are complex and difficult to characterize; thus, their microbiota have remained as a 'dark matter' of the carbon and other biogeochemical cycles. Here we deeply sequence two sediment-hosted microbial communities from an aquifer adjacent to the Colorado River, CO, USA. No single organism represents more than ∼1% of either community. Remarkably, many bacteria and archaea in these communities are novel at the phylum level or belong to phyla lacking a sequenced representative. The dominant organism in deeper sediment, RBG-1, is a member of a new phylum. On the basis of its reconstructed complete genome, RBG-1 is metabolically versatile. Its wide respiration-based repertoire may enable it to respond to the fluctuating redox environment close to the water table. We document extraordinary microbial novelty and the importance of previously unknown lineages in sediment biogeochemical transformations.

Original languageEnglish (US)
Article number2120
JournalNature Communications
Volume4
DOIs
StatePublished - 2013

Fingerprint

aquifers
Groundwater
versatility
Aquifers
Sediments
sediments
organisms
Microbiota
Archaea
Colorado River (North America)
Carbon Monoxide
Rivers
Oxidation-Reduction
water tables
biosphere
Respiration
Carbon
genome
respiration
Genome

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Castelle, C. J., Hug, L. A., Wrighton, K. C., Thomas, B. C., Williams, K. H., Wu, D., ... Banfield, J. F. (2013). Extraordinary phylogenetic diversity and metabolic versatility in aquifer sediment. Nature Communications, 4, [2120]. https://doi.org/10.1038/ncomms3120

Extraordinary phylogenetic diversity and metabolic versatility in aquifer sediment. / Castelle, Cindy J.; Hug, Laura A.; Wrighton, Kelly C.; Thomas, Brian C.; Williams, Kenneth H.; Wu, Dongying; Tringe, Susannah G.; Singer, Steven W.; Eisen, Jonathan A; Banfield, Jillian F.

In: Nature Communications, Vol. 4, 2120, 2013.

Research output: Contribution to journalArticle

Castelle, CJ, Hug, LA, Wrighton, KC, Thomas, BC, Williams, KH, Wu, D, Tringe, SG, Singer, SW, Eisen, JA & Banfield, JF 2013, 'Extraordinary phylogenetic diversity and metabolic versatility in aquifer sediment', Nature Communications, vol. 4, 2120. https://doi.org/10.1038/ncomms3120
Castelle, Cindy J. ; Hug, Laura A. ; Wrighton, Kelly C. ; Thomas, Brian C. ; Williams, Kenneth H. ; Wu, Dongying ; Tringe, Susannah G. ; Singer, Steven W. ; Eisen, Jonathan A ; Banfield, Jillian F. / Extraordinary phylogenetic diversity and metabolic versatility in aquifer sediment. In: Nature Communications. 2013 ; Vol. 4.
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