Extracellular potassium concentration regulates proliferation of immature cerebellar granule cells

Laura N Borodinsky, Mónica L. Fiszman

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The present study-examines the effect of depolarizing potassium concentrations on the proliferation of immature rat cerebellar neurons. Cells inoculated in serum free medium and 5 mM KC1 (5 K) showed a high degree of 3H-thymidine incorporation that decreased 24-48 h after plating as differentiation began. During the first 24 h after inoculation, cells grown in high potassium (25 K), showed a 34 ± 3% increase (mean ± S.E.M., n = 12) in 3H-thymidine incorporation as compared with the values observed in 5 K. After 24 h in vitro, cells grown in 25 K showed 23 ± 3% (mean ± S.E.M, n = 3) less DNA synthesis than those inoculated in 5 K. The increase in DNA synthesis due to 25 K was blocked by MgCl2 and nifedipine, but not by ω- conotoxin GVIA, suggesting that it is mediated by h a Ca2+ influx via voltage-gated calcium channels (VGCC) of the L-subtype. High potassium- induced cell proliferation was blocked by the mitogen-activated protein kinase kinase (MEK1) inhibitor (PD98059, 75 μM). The number of neurons counted after 48 h in vitro in 25 K was 35-100% above of the number obtained with 5 K and this increase also was blocked by MgCl2 and nifedipine. These data-support the hypothesis that depolarizing activity during neurogenesis plays a role in the modulation of cerebellar granule cells proliferation.

Original languageEnglish (US)
Pages (from-to)43-48
Number of pages6
JournalDevelopmental Brain Research
Volume107
Issue number1
DOIs
StatePublished - Apr 17 1998
Externally publishedYes

Fingerprint

Potassium
Magnesium Chloride
Nifedipine
Thymidine
Cell Proliferation
Conotoxins
Neurons
DNA
Serum-Free Culture Media
Mitogen-Activated Protein Kinase Kinases
Neurogenesis
Calcium Channels
In Vitro Techniques
2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one

Keywords

  • Calcium channel
  • Cerebellar granule cell
  • Depolarization
  • MAPK
  • Proliferation
  • Rat

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Extracellular potassium concentration regulates proliferation of immature cerebellar granule cells. / Borodinsky, Laura N; Fiszman, Mónica L.

In: Developmental Brain Research, Vol. 107, No. 1, 17.04.1998, p. 43-48.

Research output: Contribution to journalArticle

@article{894c7027fa6147be9a3fda40b9da6231,
title = "Extracellular potassium concentration regulates proliferation of immature cerebellar granule cells",
abstract = "The present study-examines the effect of depolarizing potassium concentrations on the proliferation of immature rat cerebellar neurons. Cells inoculated in serum free medium and 5 mM KC1 (5 K) showed a high degree of 3H-thymidine incorporation that decreased 24-48 h after plating as differentiation began. During the first 24 h after inoculation, cells grown in high potassium (25 K), showed a 34 ± 3{\%} increase (mean ± S.E.M., n = 12) in 3H-thymidine incorporation as compared with the values observed in 5 K. After 24 h in vitro, cells grown in 25 K showed 23 ± 3{\%} (mean ± S.E.M, n = 3) less DNA synthesis than those inoculated in 5 K. The increase in DNA synthesis due to 25 K was blocked by MgCl2 and nifedipine, but not by ω- conotoxin GVIA, suggesting that it is mediated by h a Ca2+ influx via voltage-gated calcium channels (VGCC) of the L-subtype. High potassium- induced cell proliferation was blocked by the mitogen-activated protein kinase kinase (MEK1) inhibitor (PD98059, 75 μM). The number of neurons counted after 48 h in vitro in 25 K was 35-100{\%} above of the number obtained with 5 K and this increase also was blocked by MgCl2 and nifedipine. These data-support the hypothesis that depolarizing activity during neurogenesis plays a role in the modulation of cerebellar granule cells proliferation.",
keywords = "Calcium channel, Cerebellar granule cell, Depolarization, MAPK, Proliferation, Rat",
author = "Borodinsky, {Laura N} and Fiszman, {M{\'o}nica L.}",
year = "1998",
month = "4",
day = "17",
doi = "10.1016/S0165-3806(97)00217-4",
language = "English (US)",
volume = "107",
pages = "43--48",
journal = "Developmental Brain Research",
issn = "0165-3806",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Extracellular potassium concentration regulates proliferation of immature cerebellar granule cells

AU - Borodinsky, Laura N

AU - Fiszman, Mónica L.

PY - 1998/4/17

Y1 - 1998/4/17

N2 - The present study-examines the effect of depolarizing potassium concentrations on the proliferation of immature rat cerebellar neurons. Cells inoculated in serum free medium and 5 mM KC1 (5 K) showed a high degree of 3H-thymidine incorporation that decreased 24-48 h after plating as differentiation began. During the first 24 h after inoculation, cells grown in high potassium (25 K), showed a 34 ± 3% increase (mean ± S.E.M., n = 12) in 3H-thymidine incorporation as compared with the values observed in 5 K. After 24 h in vitro, cells grown in 25 K showed 23 ± 3% (mean ± S.E.M, n = 3) less DNA synthesis than those inoculated in 5 K. The increase in DNA synthesis due to 25 K was blocked by MgCl2 and nifedipine, but not by ω- conotoxin GVIA, suggesting that it is mediated by h a Ca2+ influx via voltage-gated calcium channels (VGCC) of the L-subtype. High potassium- induced cell proliferation was blocked by the mitogen-activated protein kinase kinase (MEK1) inhibitor (PD98059, 75 μM). The number of neurons counted after 48 h in vitro in 25 K was 35-100% above of the number obtained with 5 K and this increase also was blocked by MgCl2 and nifedipine. These data-support the hypothesis that depolarizing activity during neurogenesis plays a role in the modulation of cerebellar granule cells proliferation.

AB - The present study-examines the effect of depolarizing potassium concentrations on the proliferation of immature rat cerebellar neurons. Cells inoculated in serum free medium and 5 mM KC1 (5 K) showed a high degree of 3H-thymidine incorporation that decreased 24-48 h after plating as differentiation began. During the first 24 h after inoculation, cells grown in high potassium (25 K), showed a 34 ± 3% increase (mean ± S.E.M., n = 12) in 3H-thymidine incorporation as compared with the values observed in 5 K. After 24 h in vitro, cells grown in 25 K showed 23 ± 3% (mean ± S.E.M, n = 3) less DNA synthesis than those inoculated in 5 K. The increase in DNA synthesis due to 25 K was blocked by MgCl2 and nifedipine, but not by ω- conotoxin GVIA, suggesting that it is mediated by h a Ca2+ influx via voltage-gated calcium channels (VGCC) of the L-subtype. High potassium- induced cell proliferation was blocked by the mitogen-activated protein kinase kinase (MEK1) inhibitor (PD98059, 75 μM). The number of neurons counted after 48 h in vitro in 25 K was 35-100% above of the number obtained with 5 K and this increase also was blocked by MgCl2 and nifedipine. These data-support the hypothesis that depolarizing activity during neurogenesis plays a role in the modulation of cerebellar granule cells proliferation.

KW - Calcium channel

KW - Cerebellar granule cell

KW - Depolarization

KW - MAPK

KW - Proliferation

KW - Rat

UR - http://www.scopus.com/inward/record.url?scp=0032540250&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032540250&partnerID=8YFLogxK

U2 - 10.1016/S0165-3806(97)00217-4

DO - 10.1016/S0165-3806(97)00217-4

M3 - Article

VL - 107

SP - 43

EP - 48

JO - Developmental Brain Research

JF - Developmental Brain Research

SN - 0165-3806

IS - 1

ER -