Abstract
The present study-examines the effect of depolarizing potassium concentrations on the proliferation of immature rat cerebellar neurons. Cells inoculated in serum free medium and 5 mM KC1 (5 K) showed a high degree of 3H-thymidine incorporation that decreased 24-48 h after plating as differentiation began. During the first 24 h after inoculation, cells grown in high potassium (25 K), showed a 34 ± 3% increase (mean ± S.E.M., n = 12) in 3H-thymidine incorporation as compared with the values observed in 5 K. After 24 h in vitro, cells grown in 25 K showed 23 ± 3% (mean ± S.E.M, n = 3) less DNA synthesis than those inoculated in 5 K. The increase in DNA synthesis due to 25 K was blocked by MgCl2 and nifedipine, but not by ω- conotoxin GVIA, suggesting that it is mediated by h a Ca2+ influx via voltage-gated calcium channels (VGCC) of the L-subtype. High potassium- induced cell proliferation was blocked by the mitogen-activated protein kinase kinase (MEK1) inhibitor (PD98059, 75 μM). The number of neurons counted after 48 h in vitro in 25 K was 35-100% above of the number obtained with 5 K and this increase also was blocked by MgCl2 and nifedipine. These data-support the hypothesis that depolarizing activity during neurogenesis plays a role in the modulation of cerebellar granule cells proliferation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 43-48 |
| Number of pages | 6 |
| Journal | Developmental Brain Research |
| Volume | 107 |
| Issue number | 1 |
| DOIs | |
| State | Published - Apr 17 1998 |
| Externally published | Yes |
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Keywords
- Calcium channel
- Cerebellar granule cell
- Depolarization
- MAPK
- Proliferation
- Rat
ASJC Scopus subject areas
- Developmental Biology
- Developmental Neuroscience
Cite this
Extracellular potassium concentration regulates proliferation of immature cerebellar granule cells. / Borodinsky, Laura N; Fiszman, Mónica L.
In: Developmental Brain Research, Vol. 107, No. 1, 17.04.1998, p. 43-48.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Extracellular potassium concentration regulates proliferation of immature cerebellar granule cells
AU - Borodinsky, Laura N
AU - Fiszman, Mónica L.
PY - 1998/4/17
Y1 - 1998/4/17
N2 - The present study-examines the effect of depolarizing potassium concentrations on the proliferation of immature rat cerebellar neurons. Cells inoculated in serum free medium and 5 mM KC1 (5 K) showed a high degree of 3H-thymidine incorporation that decreased 24-48 h after plating as differentiation began. During the first 24 h after inoculation, cells grown in high potassium (25 K), showed a 34 ± 3% increase (mean ± S.E.M., n = 12) in 3H-thymidine incorporation as compared with the values observed in 5 K. After 24 h in vitro, cells grown in 25 K showed 23 ± 3% (mean ± S.E.M, n = 3) less DNA synthesis than those inoculated in 5 K. The increase in DNA synthesis due to 25 K was blocked by MgCl2 and nifedipine, but not by ω- conotoxin GVIA, suggesting that it is mediated by h a Ca2+ influx via voltage-gated calcium channels (VGCC) of the L-subtype. High potassium- induced cell proliferation was blocked by the mitogen-activated protein kinase kinase (MEK1) inhibitor (PD98059, 75 μM). The number of neurons counted after 48 h in vitro in 25 K was 35-100% above of the number obtained with 5 K and this increase also was blocked by MgCl2 and nifedipine. These data-support the hypothesis that depolarizing activity during neurogenesis plays a role in the modulation of cerebellar granule cells proliferation.
AB - The present study-examines the effect of depolarizing potassium concentrations on the proliferation of immature rat cerebellar neurons. Cells inoculated in serum free medium and 5 mM KC1 (5 K) showed a high degree of 3H-thymidine incorporation that decreased 24-48 h after plating as differentiation began. During the first 24 h after inoculation, cells grown in high potassium (25 K), showed a 34 ± 3% increase (mean ± S.E.M., n = 12) in 3H-thymidine incorporation as compared with the values observed in 5 K. After 24 h in vitro, cells grown in 25 K showed 23 ± 3% (mean ± S.E.M, n = 3) less DNA synthesis than those inoculated in 5 K. The increase in DNA synthesis due to 25 K was blocked by MgCl2 and nifedipine, but not by ω- conotoxin GVIA, suggesting that it is mediated by h a Ca2+ influx via voltage-gated calcium channels (VGCC) of the L-subtype. High potassium- induced cell proliferation was blocked by the mitogen-activated protein kinase kinase (MEK1) inhibitor (PD98059, 75 μM). The number of neurons counted after 48 h in vitro in 25 K was 35-100% above of the number obtained with 5 K and this increase also was blocked by MgCl2 and nifedipine. These data-support the hypothesis that depolarizing activity during neurogenesis plays a role in the modulation of cerebellar granule cells proliferation.
KW - Calcium channel
KW - Cerebellar granule cell
KW - Depolarization
KW - MAPK
KW - Proliferation
KW - Rat
UR - http://www.scopus.com/inward/record.url?scp=0032540250&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032540250&partnerID=8YFLogxK
U2 - 10.1016/S0165-3806(97)00217-4
DO - 10.1016/S0165-3806(97)00217-4
M3 - Article
C2 - 9602050
AN - SCOPUS:0032540250
VL - 107
SP - 43
EP - 48
JO - Developmental Brain Research
JF - Developmental Brain Research
SN - 0165-3806
IS - 1
ER -