Extracellular matrix molecules and synaptic plasticity: Immunomapping of intracellular and secreted Reelin in the adult rat brain

Tania Ramos-Moreno, Maria J. Galazo, Cesar Porrero, Veronica Martinez-Cerdeno, Francisco Clascá

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Reelin, a large extracellular matrix glycoprotein, is secreted by several neuron populations in the developing and adult rodent brain. Secreted Reelin triggers a complex signaling pathway by binding lipoprotein and integrin membrane receptors in target cells. Reelin signaling regulates migration and dendritic growth in developing neurons, while it can modulate synaptic plasticity in adult neurons. To identify which adult neural circuits can be modulated by Reelin-mediated signaling, we systematically mapped the distribution of Reelin in adult rat brain using sensitive immunolabeling techniques. Results show that the distribution of intracellular and secreted Reelin is both very widespread and specific. Some interneuron and projection neuron populations in the cerebral cortex contain Reelin. Numerous striatal neurons are weakly immunoreactive for Reelin and these cells are preferentially located in striosomes. Some thalamic nuclei contain Reelin-immunoreactive cells. Double-immunolabeling for GABA and Reelin reveals that the Reelin- immunoreactive cells in the visual thalamus are the q1intrinsic thalamic interneurons. High local concentrations of extracellular Reelin selectively outline several dendrite spine-rich neuropils. Together with previous mRNA data, our observations suggest abundant axoplasmic transport and secretion in pathways such as the retino-collicular tract, the entorhino-hippocampal ('perforant') path, the lateral olfactory tract or the parallel fiber system of the cerebellum. A preferential secretion of Reelin in these neuropils is consistent with reports of rapid, activity-induced structural changes in adult brain circuits.

Original languageEnglish (US)
Pages (from-to)401-422
Number of pages22
JournalEuropean Journal of Neuroscience
Volume23
Issue number2
DOIs
StatePublished - Jan 2006
Externally publishedYes

Fingerprint

Neuronal Plasticity
Extracellular Matrix
Neurons
Brain
Neuropil
Interneurons
Perforant Pathway
Corpus Striatum
Thalamic Nuclei
Axonal Transport
Olfactory Bulb
Secretory Pathway
Dendrites
Thalamus
Integrins
Cerebral Cortex
Cerebellum
gamma-Aminobutyric Acid
Lipoproteins
Rodentia

Keywords

  • ApoE 2 receptors
  • Cerebral cortex
  • Dendritic spines
  • NMDA receptor
  • VLDL receptors

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Extracellular matrix molecules and synaptic plasticity : Immunomapping of intracellular and secreted Reelin in the adult rat brain. / Ramos-Moreno, Tania; Galazo, Maria J.; Porrero, Cesar; Martinez-Cerdeno, Veronica; Clascá, Francisco.

In: European Journal of Neuroscience, Vol. 23, No. 2, 01.2006, p. 401-422.

Research output: Contribution to journalArticle

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