TY - GEN
T1 - Extracellular matrix enriched spheroids for enhanced survival and differentiation of mesenchymal stem cells
AU - Gonzalez-Fernandez, Tomas
AU - Keown, Andrew T.
AU - Tenorio, Alejandro J.
AU - Leach, Jonathan K
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Statement of Purpose: The recapitulation of the cellular niche is of special importance to promote stem cell survival and phenotype specification following transplantation. The formation of mesenchymal stem cells (MSCs) into three-dimensional spheroids enhances their survival, trophic factor secretion, and tissue-forming potential compared to monodisperse cells (1). Moreover, cell-secreted ECM represents a more complex platform to recapitulate the cues to guide stem cell fate versus individual matrix proteins or functional motifs (2). We previously demonstrated that MSC-secreted ECM increased progenitor cell osteogenic differentiation of diverse origins and donor populations (2). Therefore, we hypothesized that the formation of MSC spheroids enriched with cell-secreted ECM would enhance MSC viability, proliferation and differentiation potential.
AB - Statement of Purpose: The recapitulation of the cellular niche is of special importance to promote stem cell survival and phenotype specification following transplantation. The formation of mesenchymal stem cells (MSCs) into three-dimensional spheroids enhances their survival, trophic factor secretion, and tissue-forming potential compared to monodisperse cells (1). Moreover, cell-secreted ECM represents a more complex platform to recapitulate the cues to guide stem cell fate versus individual matrix proteins or functional motifs (2). We previously demonstrated that MSC-secreted ECM increased progenitor cell osteogenic differentiation of diverse origins and donor populations (2). Therefore, we hypothesized that the formation of MSC spheroids enriched with cell-secreted ECM would enhance MSC viability, proliferation and differentiation potential.
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M3 - Conference contribution
AN - SCOPUS:85065429116
T3 - Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
BT - Society for Biomaterials Annual Meeting and Exposition 2019
PB - Society for Biomaterials
T2 - 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
Y2 - 3 April 2019 through 6 April 2019
ER -