Abstract
Statement of Purpose: The recapitulation of the cellular niche is of special importance to promote stem cell survival and phenotype specification following transplantation. The formation of mesenchymal stem cells (MSCs) into three-dimensional spheroids enhances their survival, trophic factor secretion, and tissue-forming potential compared to monodisperse cells (1). Moreover, cell-secreted ECM represents a more complex platform to recapitulate the cues to guide stem cell fate versus individual matrix proteins or functional motifs (2). We previously demonstrated that MSC-secreted ECM increased progenitor cell osteogenic differentiation of diverse origins and donor populations (2). Therefore, we hypothesized that the formation of MSC spheroids enriched with cell-secreted ECM would enhance MSC viability, proliferation and differentiation potential.
| Original language | English (US) |
|---|---|
| Title of host publication | Society for Biomaterials Annual Meeting and Exposition 2019 |
| Subtitle of host publication | The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting |
| Publisher | Society for Biomaterials |
| Number of pages | 1 |
| ISBN (Electronic) | 9781510883901 |
| State | Published - Jan 1 2019 |
| Event | 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States Duration: Apr 3 2019 → Apr 6 2019 |
Publication series
| Name | Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium |
|---|---|
| Volume | 40 |
| ISSN (Print) | 1526-7547 |
Conference
| Conference | 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence |
|---|---|
| Country | United States |
| City | Seattle |
| Period | 4/3/19 → 4/6/19 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Biotechnology
- Biomaterials
- Materials Chemistry
Cite this
Extracellular matrix enriched spheroids for enhanced survival and differentiation of mesenchymal stem cells. / Gonzalez-Fernandez, Tomas; Keown, Andrew T.; Tenorio, Alejandro J.; Leach, Jonathan K.
Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials, 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Extracellular matrix enriched spheroids for enhanced survival and differentiation of mesenchymal stem cells
AU - Gonzalez-Fernandez, Tomas
AU - Keown, Andrew T.
AU - Tenorio, Alejandro J.
AU - Leach, Jonathan K
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Statement of Purpose: The recapitulation of the cellular niche is of special importance to promote stem cell survival and phenotype specification following transplantation. The formation of mesenchymal stem cells (MSCs) into three-dimensional spheroids enhances their survival, trophic factor secretion, and tissue-forming potential compared to monodisperse cells (1). Moreover, cell-secreted ECM represents a more complex platform to recapitulate the cues to guide stem cell fate versus individual matrix proteins or functional motifs (2). We previously demonstrated that MSC-secreted ECM increased progenitor cell osteogenic differentiation of diverse origins and donor populations (2). Therefore, we hypothesized that the formation of MSC spheroids enriched with cell-secreted ECM would enhance MSC viability, proliferation and differentiation potential.
AB - Statement of Purpose: The recapitulation of the cellular niche is of special importance to promote stem cell survival and phenotype specification following transplantation. The formation of mesenchymal stem cells (MSCs) into three-dimensional spheroids enhances their survival, trophic factor secretion, and tissue-forming potential compared to monodisperse cells (1). Moreover, cell-secreted ECM represents a more complex platform to recapitulate the cues to guide stem cell fate versus individual matrix proteins or functional motifs (2). We previously demonstrated that MSC-secreted ECM increased progenitor cell osteogenic differentiation of diverse origins and donor populations (2). Therefore, we hypothesized that the formation of MSC spheroids enriched with cell-secreted ECM would enhance MSC viability, proliferation and differentiation potential.
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UR - http://www.scopus.com/inward/citedby.url?scp=85065429116&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85065429116
T3 - Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
BT - Society for Biomaterials Annual Meeting and Exposition 2019
PB - Society for Biomaterials
ER -