Extracellular Matrix-Coated Composite Scaffolds Promote Mesenchymal Stem Cell Persistence and Osteogenesis

Jenna N. Harvestine, Nina L. Vollmer, Steve S. Ho, Christopher A. Zikry, Mark A Lee, Jonathan K Leach

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Composite scaffolds of bioactive glass and poly(lactide-co-glycolide) provide advantages over homogeneous scaffolds, yet their therapeutic potential can be improved by strategies that promote adhesion and present instructive cues to associated cells. Mesenchymal stem cell (MSC)-secreted extracellular matrix (ECM) enhances survival and function of associated cells. To synergize the benefits of an instructive ECM with composite scaffolds, we tested the capacity of ECM-coated composite scaffolds to promote cell persistence and resultant osteogenesis. Human MSCs cultured on ECM-coated scaffolds exhibited increased metabolic activity and decreased apoptosis compared to uncoated scaffolds. Additionally, MSCs on ECM-coated substrates in short-term culture secreted more proangiogenic factors while maintaining markers of osteogenic differentiation. Upon implantation, we detected improved survival of MSCs on ECM-coated scaffolds over 3 weeks. Histological evaluation revealed enhanced cellularization and osteogenic differentiation in ECM-coated scaffolds compared to controls. These findings demonstrate the promise of blending synthetic and natural ECMs and their potential in tissue regeneration.

Original languageEnglish (US)
Pages (from-to)3524-3531
Number of pages8
JournalBiomacromolecules
Volume17
Issue number11
DOIs
StatePublished - Nov 14 2016

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Stem cells
Scaffolds
Composite materials
Scaffolds (biology)
Military electronic countermeasures
Tissue regeneration
Bioactive glass
Polyglactin 910
Cell death
Adhesion
Apoptosis
Substrates

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Extracellular Matrix-Coated Composite Scaffolds Promote Mesenchymal Stem Cell Persistence and Osteogenesis. / Harvestine, Jenna N.; Vollmer, Nina L.; Ho, Steve S.; Zikry, Christopher A.; Lee, Mark A; Leach, Jonathan K.

In: Biomacromolecules, Vol. 17, No. 11, 14.11.2016, p. 3524-3531.

Research output: Contribution to journalArticle

Harvestine, Jenna N. ; Vollmer, Nina L. ; Ho, Steve S. ; Zikry, Christopher A. ; Lee, Mark A ; Leach, Jonathan K. / Extracellular Matrix-Coated Composite Scaffolds Promote Mesenchymal Stem Cell Persistence and Osteogenesis. In: Biomacromolecules. 2016 ; Vol. 17, No. 11. pp. 3524-3531.
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