Extracellular Engrailed Participates in the Topographic Guidance of Retinal Axons In Vivo

Andrea Wizenmann, Isabelle Brunet, Joyce S Y Lam, Laure Sonnier, Marine Beurdeley, Konstantinos Zarbalis, Daniela Weisenhorn-Vogt, Christine Weinl, Asha Dwivedy, Alain Joliot, Wolfgang Wurst, Christine Holt, Alain Prochiantz

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Engrailed transcription factors regulate the expression of guidance cues that pattern retinal axon terminals in the dorsal midbrain. They also act directly to guide axon growth in vitro. We show here that an extracellular En gradient exists in the tectum along the anterior-posterior axis. Neutralizing extracellular Engrailed in vivo with antibodies expressed in the tectum causes temporal axons to map aberrantly to the posterior tectum in chick and Xenopus. Furthermore, posterior membranes from wild-type tecta incubated with anti-Engrailed antibodies or posterior membranes from Engrailed-1 knockout mice exhibit diminished repulsive activity for temporal axons. Since EphrinAs play a major role in anterior-posterior mapping, we tested whether Engrailed cooperates with EphrinA5 in vitro. We find that Engrailed restores full repulsion to axons given subthreshold doses of EphrinA5. Collectively, our results indicate that extracellular Engrailed contributes to retinotectal mapping in vivo by modulating the sensitivity of growth cones to EphrinA.

Original languageEnglish (US)
Pages (from-to)355-366
Number of pages12
JournalNeuron
Volume64
Issue number3
DOIs
StatePublished - Nov 12 2009
Externally publishedYes

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Axons
Growth Cones
Membranes
Presynaptic Terminals
Mesencephalon
Xenopus
Knockout Mice
Cues
Anti-Idiotypic Antibodies
Transcription Factors
Axon Guidance
Antibodies
Growth

Keywords

  • CELLBIO
  • DEVBIO
  • MOLNEURO

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Wizenmann, A., Brunet, I., Lam, J. S. Y., Sonnier, L., Beurdeley, M., Zarbalis, K., ... Prochiantz, A. (2009). Extracellular Engrailed Participates in the Topographic Guidance of Retinal Axons In Vivo. Neuron, 64(3), 355-366. https://doi.org/10.1016/j.neuron.2009.09.018

Extracellular Engrailed Participates in the Topographic Guidance of Retinal Axons In Vivo. / Wizenmann, Andrea; Brunet, Isabelle; Lam, Joyce S Y; Sonnier, Laure; Beurdeley, Marine; Zarbalis, Konstantinos; Weisenhorn-Vogt, Daniela; Weinl, Christine; Dwivedy, Asha; Joliot, Alain; Wurst, Wolfgang; Holt, Christine; Prochiantz, Alain.

In: Neuron, Vol. 64, No. 3, 12.11.2009, p. 355-366.

Research output: Contribution to journalArticle

Wizenmann, A, Brunet, I, Lam, JSY, Sonnier, L, Beurdeley, M, Zarbalis, K, Weisenhorn-Vogt, D, Weinl, C, Dwivedy, A, Joliot, A, Wurst, W, Holt, C & Prochiantz, A 2009, 'Extracellular Engrailed Participates in the Topographic Guidance of Retinal Axons In Vivo', Neuron, vol. 64, no. 3, pp. 355-366. https://doi.org/10.1016/j.neuron.2009.09.018
Wizenmann, Andrea ; Brunet, Isabelle ; Lam, Joyce S Y ; Sonnier, Laure ; Beurdeley, Marine ; Zarbalis, Konstantinos ; Weisenhorn-Vogt, Daniela ; Weinl, Christine ; Dwivedy, Asha ; Joliot, Alain ; Wurst, Wolfgang ; Holt, Christine ; Prochiantz, Alain. / Extracellular Engrailed Participates in the Topographic Guidance of Retinal Axons In Vivo. In: Neuron. 2009 ; Vol. 64, No. 3. pp. 355-366.
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