Extracellular cyclic adenosine monophosphate-dependent protein kinase A autoantibody and C-reactive protein as serum biomarkers for diagnosis of cancer in dogs

Min Ok Ryu, Byung Gak Kim, Ul Soo Choi, Kwan Hyuck Baek, Young Ki Song, Qiang Li, Kyoung Won Seo, Sandra Ryeom, Hwa Young Youn, Dong Ha Bhang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Protein kinase A, a cyclic adenosine monophosphate (AMP)-dependent enzyme, normally exists within mammalian cells; however, in cancer cells, it can leak out and be found in the serum. Extracellular cyclic AMP-dependent protein kinase A (ECPKA) has been determined to increase in the serum of cancer-bearing dogs. However, there have been no reports in the veterinary literature on serum ECPKA autoantibody (ECPKA-Ab) expression in dogs with cancer. The aim of this study was to evaluate ECPKA-Ab and C-reactive protein (CRP) as serum biomarkers for cancer in dogs. ECPKA-Ab and CRP levels were detected by an enzyme-linked immunosorbent assay in serum samples from dogs with malignant tumours (n = 167), benign tumours (n = 42), or non-tumour disease (n = 155) and from healthy control dogs (n = 123). ECPKA-Ab and CRP levels were significantly higher in the dogs with malignant tumours than in those with benign tumours or non-tumour diseases, as well as in the healthy controls (P < 0.001, Kruskal-Wallis test). There was a significant positive correlation between the neoplastic index, which was developed using ECPKA-Ab and CRP levels, and the presence of cancer in dogs (P < 0.001); the area under the receiver-operating characteristic curve was estimated to be >0.85 (P < 0.001). In conclusion, ECPKA-Ab is a potential serum biomarker for a broad spectrum of cancers. Combined measurement of CRP and ECPKA-Ab levels in serum improves the sensitivity and accuracy of a diagnosis of cancer in dogs.

Original languageEnglish (US)
JournalVeterinary and Comparative Oncology
DOIs
StateAccepted/In press - Jan 1 2018
Externally publishedYes

Fingerprint

autoantibodies
cAMP-dependent protein kinase
C-reactive protein
cyclic AMP
Tumor Biomarkers
Cyclic AMP-Dependent Protein Kinases
Cyclic AMP
C-Reactive Protein
Autoantibodies
Protein Kinase C
biomarkers
Dogs
neoplasms
dogs
Serum
Neoplasms
enzyme-linked immunosorbent assay

Keywords

  • autoantibody
  • biomarker
  • C-reactive protein
  • cancer
  • extracellular protein kinase A

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Extracellular cyclic adenosine monophosphate-dependent protein kinase A autoantibody and C-reactive protein as serum biomarkers for diagnosis of cancer in dogs. / Ryu, Min Ok; Kim, Byung Gak; Choi, Ul Soo; Baek, Kwan Hyuck; Song, Young Ki; Li, Qiang; Seo, Kyoung Won; Ryeom, Sandra; Youn, Hwa Young; Bhang, Dong Ha.

In: Veterinary and Comparative Oncology, 01.01.2018.

Research output: Contribution to journalArticle

Ryu, Min Ok ; Kim, Byung Gak ; Choi, Ul Soo ; Baek, Kwan Hyuck ; Song, Young Ki ; Li, Qiang ; Seo, Kyoung Won ; Ryeom, Sandra ; Youn, Hwa Young ; Bhang, Dong Ha. / Extracellular cyclic adenosine monophosphate-dependent protein kinase A autoantibody and C-reactive protein as serum biomarkers for diagnosis of cancer in dogs. In: Veterinary and Comparative Oncology. 2018.
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AU - Ryu, Min Ok

AU - Kim, Byung Gak

AU - Choi, Ul Soo

AU - Baek, Kwan Hyuck

AU - Song, Young Ki

AU - Li, Qiang

AU - Seo, Kyoung Won

AU - Ryeom, Sandra

AU - Youn, Hwa Young

AU - Bhang, Dong Ha

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AB - Protein kinase A, a cyclic adenosine monophosphate (AMP)-dependent enzyme, normally exists within mammalian cells; however, in cancer cells, it can leak out and be found in the serum. Extracellular cyclic AMP-dependent protein kinase A (ECPKA) has been determined to increase in the serum of cancer-bearing dogs. However, there have been no reports in the veterinary literature on serum ECPKA autoantibody (ECPKA-Ab) expression in dogs with cancer. The aim of this study was to evaluate ECPKA-Ab and C-reactive protein (CRP) as serum biomarkers for cancer in dogs. ECPKA-Ab and CRP levels were detected by an enzyme-linked immunosorbent assay in serum samples from dogs with malignant tumours (n = 167), benign tumours (n = 42), or non-tumour disease (n = 155) and from healthy control dogs (n = 123). ECPKA-Ab and CRP levels were significantly higher in the dogs with malignant tumours than in those with benign tumours or non-tumour diseases, as well as in the healthy controls (P < 0.001, Kruskal-Wallis test). There was a significant positive correlation between the neoplastic index, which was developed using ECPKA-Ab and CRP levels, and the presence of cancer in dogs (P < 0.001); the area under the receiver-operating characteristic curve was estimated to be >0.85 (P < 0.001). In conclusion, ECPKA-Ab is a potential serum biomarker for a broad spectrum of cancers. Combined measurement of CRP and ECPKA-Ab levels in serum improves the sensitivity and accuracy of a diagnosis of cancer in dogs.

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